Microscopes within the BALM facility

A video of the Nikon A1R Inverted Confocal/TIRF in use by one of our PhD students.

 
The following is a selection of the microscope facilities available:

Leica TCS SP2

The Leica SP2 system is a laser scanning confocal system equipped with a DMRE2 inverted microscope, AOBS spectral detector and a full in vivo imaging set up (T- and CO2- controlled within an incubation chamber). The system feautures 6 laser lines (405- 476- 488- 514- 546- 647nm), widefield detector with DIC for simultaneous detection of confocal and transmitted light images, time-lapse imaging of live samples, FRAP/FRET imaging. The SP2 system is located on the 6th floor of the Biosciences building.

 

Nikon A1R Inverted Confocal/TIRF

This Nikon system is a dual TIRF and laser scanning confocal (A1R) system, equipped with a Ti inverted microscope, Perfect Focus System (PFS), piezo-z, multi laser beds, a full in vivo imaging set up (T- and CO2- controlled within an incubation chamber) and an inverted motorized stage suitable for live imaging of cells (well plates or Matek dishes).

The A1R laser scanning confocal system feautures, spectral detectors, 7 laser lines (405- 457- 476- 488- 514- 546- 633nm), a resonant scanner for fast processes in live cells, live ratiometric imaging, tiled imaging optical sectioning for specimens that extend beyond the field of view (e.g. plants, invertebrates, neurons, microfluidic devices), widefield detector with DIC for simultaneous detection of confocal and transmitted light images; time-lapse imaging module and lambda imaging for spectral characterisation.

The TIRF system feautures 3 laser lines (488- 546- 647nm), an Andor EMCCD camera and 2 powerful TIRF lenses. The Nikon system is located on the 8th floor of the Biosciences building.

Nikon Eclipse E600

Nikon E600The Nikon E600 is an upright widefield/epi-fluorescent microscope equipped with an Hamamatsu CCD camera and controlled by NIS-Elements acquisition software. The system is located on the 8th floor of the Biosciences building. 

 

Nikon SPZ1000

nikon-spz1000This is a fluorescent stereo/dissecting microscope equipped with an Nikon DS-Fi1 camera and controlled by NIS-Elements acquisition software. The system includes and anti-vibration platform to allow minimal impact on dissection and micromanipulation and a 488nm filter. The system is located on the 8th floor of the Biosciences building.

 

Olympus inverted widefield/epi-fluorescent microscope

olympus-ix70Located on the 1st floor the Olympus IX70 epi-fluorescent microscope is equipped with a halogen light source and a Hamamatsu CCD camera controlled by PC/software for imaging acquisition and analsys.

Olympus Widefield/TIRF

This Olympus system is an inverted widefield/TIRF laser scanning confocal system equipped with a IX81 inverted microscope, and a full in vivo imaging set up (T- and CO2- controlled within an incubation chamber). The system feautures 2 powerful TIRF lenses, a dual view module for multicolour TIRF applications, an Hamamatsu ImageEM camera, 3 solid state lasers (491- 560- 640nm) and inverted motorized stage (suitable for live imaging of cells on well plates or Matek dishes), widefield detector with DIC for simultaneous detection of confocal and transmitted light images. The system is located on the 8th floor of the Biosciences building.

Zeiss LSM 710 Confocor 3

Zeiss-LSM710Located on the 8th floor of Biosciences. Laser scanning confocal microscope with integrated fluorescence correlation spectroscopy (FCS)

Zeiss widefield/epi-fluorescent optogenetic system (upright)

zeiss-epi-flourescentMotorised Axio Imager.M2 geared toward fluorescence performance with LED excitation for optogentics research delivering optical control signal at high speed and with the precision (cell type–specific) required for biological processing. The system is equipped with the Zeiss Colibri.2 LED illumination system (four channels are included at 365nm, 470nm, 565nm and 590nm), a motorized switching prism and a fluorescence triple band pass filter set.

Included in the system there is a Photometrics OptiMos high speed, high sensitivity moderate resolution sCMOS camera (with associated drivers and triggering system) and a second high resolution, high sensitivity, moderate speed Axiocam 506 colour microscopy camera from Carl Zeiss. The system is located on the 8th floor of the School of Biosciences.

Nikon Eclipse Ti2000

eclipse-ti2000The Nikon Ti2000 is an inverted epi-fluorescent microscope equipped with a full live imaging set up (T- and CO2-controlled within an incubation chamber), Cairn LED system and Photometrics CCD camera. The microscope is semi-automated and controlled by MicroManager software. The system is located on the 1st floor of the Biosciences building.

Leica DMRE

leica-dmreThe Leica DMRE is an upright widefield/epi-fluorescent microscope equipped with an Hamamatsu OrcaR2 CCD camera and controlled by RCImage acquisition software. The system is located on the 6th floor of the Biosciences building.

Leica TCS SP8

leica-tcs-sp8The Leica SP8 system is an upright laser scanning confocal system equipped with a DM6 upright microscope, AOBS spectral detector and a full in vivo imaging set up (T- and CO2- controlled within an incubation chamber). The system feautures 8 laser lines (405- 458- 476- 488- 496- 514- 561- 647nm),, a resonant scanner for fast processes in live cells, live ratiometric imaging, calcium imaging, a Hy-D detector for low phototoxicity, low signal probes and high resolution imaging; glycerol immersion objective for preservation of axial resolution when imaging in 3D, and water dipping lenses for live imaging.

Other features include: tiled imaging optical sectioning of specimens that extend beyond the field of view, e.g. plants, invertebrates, neurons, microfluidic devices; upright stand suitable for live imaging; widefield detector with DIC for simultaneous detection of confocal and transmitted light images; on-board 3D viewer and analysis software; on-board Spectral unmixing for the spectral deconvolution of multiple overlapping fluorscence; time-lapse imaging of live samples; lambda imaging for spectral characterisation.

The system is located on the 6th floor of the Biosciences building. 

In addition to training and technical assistance we also provide data analysis capabilities through our dedicated workstation

Imaging Workstations

 

Two PC computers are also available at no charge for imaging analisys. The software included are:

  • Fiji-ImageJ
  • NIS Elements 4.13
  • ZEN 2012
  • Leica LASX
  • ICY
  • MotionTracking