Microscopes within the BALM facility

A video of the Nikon A1R Inverted Confocal/TIRF in use by one of our PhD students.

 
The following is a selection of the microscope facilities available:

Leica SP2 Upright Confocal

Leica SP2 Confocal system located on the 6th floor of Biosciences building. The DMIRE2 inverted microscope is equipped with spectral detector and environmental chamber, mercury and halogen lamps and is linked to UV diode laser, ArKr laser and He/Ne lasers.  

 

Leica SP2 Upright Confocal

Leica SP2Leica SP2 Confocal system located on the 6th floor of Biosciences building. The DMIRE2 inverted microscope is equipped with mercury a lamp and is linked to ArKr lasers.

 

Leica SP2 Inverted Confocal

Leica SP2 Inverted Confocal on 6th floor of Biosciences with spectral detector and environmental chamber

 

Nikon A1R Inverted Confocal/TIRF

Nikon A1R Inverted Confocal/TIRF on 8th floor of Biosciences with resonant scanner, environmental chamber, spectral detector, EMCCD, 'Perfect Focus System' and motorised XYZ (including piezo-Z)

 

Nikon Eclipse epi-fluorescent microscope

Nikon E600Nikon Eclipse E600 epi-fluorescent microscope equipped with an Hamamatsu CCD camera controlled by PC/software. The microscope is located on the 6th floor of the Bioscience building.

 

Nikon fluorescent stereo/dissecting microscope

nikon-spz1000The Nikon SPZ1000 fluorescent stereo microscope is equipped with an anti-vibration station to allow minimal impact on dissections and micromanipulations. Together with a halogen light source, the microscope is equipped with a Nikon digital colour camera DS-Fi1 and acquisition software. The system is located on the 8th floor Bioscience.

 

Olympus inverted widefield/epi-fluorescent microscope

olympus-ix70Located on the 1st floor the Olympus IX70 epi-fluorescent microscope is equipped with a halogen light source and a Hamamatsu CCD camera controlled by PC/software for imaging acquisition and analsys.

Olympus Widefield/TIRF

Olympus IX81 inverted widefield/epifluorescent/TIRF located on the 8th of the Bioscience building equipped with 491, 560, 640 lasers, environmentally control chamber, motorised stage and two Hamamatsu CCD cameras (Orca R2 and high-speed ImageEm).

Zeiss LSM 710 Confocor 3

Zeiss-LSM710Located on the 8th floor of Biosciences. Laser scanning confocal microscope with integrated fluorescence correlation spectroscopy (FCS)

Zeiss widefield/epi-fluorescent optogenetic system (upright)

zeiss-epi-flourescentMotorised Axio Imager.M2 geared toward fluorescence performance with LED excitation for optogentics research delivering optical control signal at high speed and with the precision (cell type–specific) required for biological processing. The system is equipped with the Zeiss Colibri.2 LED illumination system (four channels are included at 365nm, 470nm, 565nm and 590nm), a motorized switching prism and a fluorescence triple band pass filter set.

Included in the system there is a Photometrics OptiMos high speed, high sensitivity moderate resolution sCMOS camera (with associated drivers and triggering system) and a second high resolution, high sensitivity, moderate speed Axiocam 506 colour microscopy camera from Carl Zeiss. The system is located on the 8th floor of the School of Biosciences.

In addition to training and technical assistance we also provide data analysis capabilities through our dedicated workstation

Imaging Workstations

 

Two PC computers are also available at no charge for imaging analisys. The software included are:

  • Fiji-ImageJ
  • NIS Elements 4.13
  • ZEN 2012
  • Leica LCS
  • ICY
  • Amira