HEp-2: Cytoplasmic fibres

Three types of cytoskeletal fibres are recognised; these include microtubules, intermediate filaments and microfilaments.

Actin

Antigen Actin, vimentin & tubulin

Clinical Chronic active hepatitis, dermatomyositis

Antigen Cytokeratin 8, 18 and 19

Clinical Non-specific

Antigen Tropomyosin

Clinical Non-specific

**Vimentin**
Antigen: Vimentin Clinical: Non-specific
Characteristic dots in the cytoplasm of the mitotic cells can been seen with vimentin antibody (shown by an arrow on the larger left-hand side image).