HEp-2: Cytoplasmic fibres

Three types of cytoskeletal fibres are recognised; these include microtubules, intermediate filaments and microfilaments.

Actin


Antigen
Actin, vimentin & tubulin

Clinical Chronic active hepatitis, dermatomyositis


Cytokeratin


Antigen
Cytokeratin 8, 18 and 19 

Clinical Non-specific

Tropomyosin


Antigen
Tropomyosin

Clinical Non-specific

Vimentin

Vimentin


Antigen: Vimentin

Clinical: Non-specific

Characteristic dots in the cytoplasm of the mitotic cells can been seen with vimentin antibody (shown by an arrow on the larger left-hand side image).