HEp-2: Mitotics related Ab

The antigens are expressed in the nuclei of the cells in the S-phase hence the staining is confined to the dividing cells.

HEp2 metaphase: Centriole

Mitotic antibody
HEp2 cells

Usually only one dot is seen but the second dot appears in teh s-phase.
Antigen: Centriole/centrosome proteins
Clinical: Raynaud's phenomenon, scleroderma, Sjögren's syndrome and CREST.


HEp2 metaphase: MSA-1 (mitotic spindle type 1)

Mitotic antibody
HEp2 cells


Antigen:
 NuMA
Clinical: Uncommon clinically non-specific pattern.


HEp2 metaphase: MSA-2 (midbody, mitotic spindle type 2)

 
Mitotic antibody
HEp2 cells


Antigen:
 Fibrillar synthesis protein of nuclear membrane. The binding of the antibody is confined to the cleavage furrow.
Clinical: RA, inflammatory conditions


Below are two variation of antibodies to Proliferating Cell Nuclear Antigens (PCNA) which are expressed in the nuclei of cells during DNA synthesis phase of the cell cycle.

HEp2 metaphase: Pleomorphic speckled (PCNA)

Mitotic antibody
HEp2 cells


Antigen:
 Cyclin I, The pattern of staining ranges from fine to coarse speckled. The mitotic in cyclin type 1 are negative compared to cyclin II
Clinical: SLE, lymphoma, lymphoproliferative disease.


HEp2 metaphase: Cyclin II

Mitotic antibody
HEp2 cells


Antigen:
Cyclin II, similar to cyclin I except for the presence of dots in the mitotics.
Clinical: Various carcinomas .