Nuclear without mitotic staining

As mentioned earlier, pattern recognition is based on examining the binding of auto-antibodies to Human epithelial type 2 (HEp-2) cells at the interphase and the dividing stages (metaphase) of the cells. The combination of the reactions seen at these stages defines the pattern. The react of the antinuclear antibodies presented here is confined to interphase only and no reaction is seen in metaphase.

ANA: Nuclear rim (membranous)

ANA
HEp2 cells

Much finer rim than the above. Homogenous staining of the nuclear membrane forming a rim pattern. Folding of the membrane is characteristic of this pattern. 
Antigen: Lamins A, B1, B2, C
Clinical: Mixed chronic disorders, chronic fatigue syndrome

ANA: Nuclear membrane pores

ANA
HEp2 cells
Nuclear membrane surface dots when adjusting fine focus. 
Antigen: gp210, nucleoporin p62
Clinical: PBC and polymyositis

ANA: Nuclear Matrix

ANA
HEp2 cells
Large, variable size, speckles arranged in sponge-like pattern (often referred to as "crazy paving")
Antigen: Heterogenous ribonuclear proteins (hnRNP)
Clinical: SLE, mixed connective tissue disease, rheumatoid arthritis & scleroderma

ANA:Coarse speckled

ANA
Dense intermediate sized speckling of the nucleus.
HEp2 cells


Antigens
Sm
U1-snRNP U2-snRNP
U4/U6-snRNP

Clinical
SLE (5-30%)
SLE (15-40%), SLE, MCTD (95-100%)
SLE, MCTD, Raynaud's phenomenon, Psoriasis,
Sjögren's syndrome and systemic sclerosis

ANA: Fine speckled

ANA
Fine granular (uniform) staining of the HEp-2 nucleus.
HEp2 Cells
Antigens
 
Mi-2
SSA/Ro, SSB/La,
RNA polymerase II

Clinical
Dermatomyositis
Sjögren's syndrome, SLE, congenital heart block
Systemic Sclerosis

ANA: Few nuclear dots

ANA
Up to six dots in the nuclei.
HEp2 Cells

Antigens
 
Mi-2 
p80 coilin

Clinical
Primary biliary cirrhosis (30%),
chronic active hepatitis

ANA: Multiple nuclear dots

ANA
Between 6 and 10 dots in the nuclei of the HEp-2 cells.
HEp2 Cells


Antigens
 
Sp 100

Clinical
Primary biliary cirrhosis (6%),
Sjögren's syndrome, SLE

 MCTD = Mixed Connective Tissue Disease