Metabolite identification with the Q Exactive and LTQ Orbitrap

This 2-day course will provide a hands-on approach to teach the attendees about the latest techniques and tools available to perform metabolite identification in non-targeted metabolomics studies. The course will be led by experts working within the fields of metabolomics and chemical analysis and will include a significant proportion of hands-on experience of using mass spectrometers, software tools and databases. A maximum of four people will be working on each mass spectrometer in a session. We will apply these tools on the Q Exactive and LTQ-Orbitrap family of mass spectrometers.

Topics covered:

  • Importance of mass spectral interpretation
  • Types of data which can be collected on the QE and LTQ-Orbitrap (m/z, retention time, MS/MS, MSn)
  • Conversion of raw data to molecular formula and putative metabolite annotations
  • MS/MS experiments in metabolic phenotyping for on-line data acquisition using the QE (DDA, DIA, all-ion)
  • MS/MS and MSn experiments for sample fractions using the LTQ-Orbitrap
  • Mass spectral libraries (using mzCloud)
  • Searching mass spectral libraries
  • Tools for mass spectral interpretation
  • Reporting standards for metabolite identification
  • Question and answer session with the experts

Download the full programme here

Dates: 19-20 July 2017, 14-15 December 2017

NERC-funded bursaries: A limited number of NERC-funded bursaries are available for PhD students to attend the course. If you are a PhD student funded by NERC please select the bursary option when registering for the course. You will be notified by email if you have been awarded a bursary to attend the course. The bursary will cover the full cost of the course, accommodation (up to 2 nights, which we will book for you) and up to £100 towards travel costs.

ELIXIR-UK funded bursaries:  A limited number of ELIXIR-UK funded bursaries are available for PhD students funded by either the BBSRC or MRC to attend the course. If you are a PhD student funded by the BBSRC or MRC please select the bursary option when registering for the course. You will be notified by email if you have been awarded a bursary to attend the course. The bursary will cover the course registration fee, you will need to pay for your own accommodation and travel costs.

Cost: Early bird £755, standard £840

Level: The course is aimed at PhD students and post-doctoral researchers who have been actively applying metabolomics for a minimum of 6 months. If students or researchers would like to take the course but do not have the recommended level of experience please contact the course administrator for advice.

Register for the July course here 

Register for the December course here

For further information contact David Epps. Please read the Birmingham Metabolomics Training Centre’s Conditions and Cancellation Policy  prior to registering.