This 2-day course will provide a hands-on approach to teach the attendees about the latest techniques and tools available to perform metabolite identification in non-targeted metabolomics studies. The course will be led by experts working within the fields of metabolomics and chemical analysis and will include a significant proportion of hands-on experience of using mass spectrometers, software tools and databases. A maximum of four people will be working on each mass spectrometer in a session. We will apply these tools on the Q Exactive and LTQ-Orbitrap family of mass spectrometers.
- Importance of mass spectral interpretation
- Types of data which can be collected on the QE and LTQ-Orbitrap (m/z, retention time, MS/MS, MSn)
- Conversion of raw data to molecular formula and putative metabolite annotations
- MS/MS experiments in metabolic phenotyping for on-line data acquisition using the QE (DDA, DIA, all-ion)
- MS/MS and MSn experiments for sample fractions using the LTQ-Orbitrap
- Mass spectral libraries (using mzCloud)
- Searching mass spectral libraries
- Tools for mass spectral interpretation
- Reporting standards for metabolite identification
- Question and answer session with the experts
Download the full programme here
Date: 15-16 May 2017
Cost: Early bird £755, standard £840
Level: The course is aimed at PhD students and post-doctoral researchers who have been actively applying metabolomics for a minimum of 6 months. If students or researchers would like to take the course but do not have the recommended level of experience please contact the course administrator for advice.
For further information or to register your interest in the course contact David Epps
Please read the Birmingham Metabolomics Training Centre’s Conditions and Cancellation Policy prior to registering.