Experiments

Pulse sequences are available for assigning, structural analysis and screening of proteins, lipids, nucleic acids and oligosaccharides, metabolic profiling and DNP studies of biofluids and mixtures.

The following is a list of some of the available NMR experiments.

Pleaseinquire if you are interested in a specific experiment not listed.

Assignment: 15N-resolved for protein assignment

  • 15N-edited TOCSY
  • C(CO)NH
  • H(CO)NH
  • CBCA(CO)NH CBCANH
  • (HCA)CO(CA)NH HNCA
  • HNCA_CT
  • HNCO HN(CA)CO
  • HNCACB
  • HCA(CO)N
  • HN(CO)CA

Assignment: 13C-resolved for protein assignmnent

  • HCACO
  • HCCH-COSY
  • HCCH-TOCSY

Calibration of pulses

  • 13C
  • 15N

Coupling constants

  • HMQC-J
  • HNHA

Diffusion

  • DOSY
  • waterSLED

Dynamic nuclear polarization

Ligand Screening

  • SALMON
  • STD-NMR
  • waterLOGSY

Metabolomics

  • J-resolved
  • HADAMARD TOCSY

Projection Angle Reconstruction

Relaxation

  • T1 Relaxation
  • T1rho Relaxation
  • T2 Relaxation

Residual Dipolar Coupling

  • 15N-HSQC-IPAP

Structure Determination

  • NOESY, 15N-edited (2D, 3D)
  • NOESY, 13C-edited (3D, 4D)
  • ROESY

TROSY

  • TROSY, 15N-1H correlation
  • (H)CCH TROSY (3D)
  • TROSY-NOESY (2D and 3D)
  • HNCA (3D)
  • HNCACB (3D)

The Varian Inc BioPack of experiments is installed on all Varian NMR systems.

 

 HWB NMR logoNMR spectrum of a PhosphataseCustom pulse sequences tailored to user needs as well as standardized pulse sequences libraries are installed on all our NMR spectrometers.