Laboratory

We offer users a 45 sqm wet-lab stocked with the consumables and equipment needed for NMR sample preparation; access to other biophysical tools can also be arranged.

Equipment available in the NMR laboratory

  • centrifuges for NMR sample tubes and eppendorf tubes
  • chemical fumehood
  • dryer for tubes
  • freezers (-20º and -80ºC)
  • pH meter for NMR sample tubes
  • refrigerators t
  • ube cleaner

Consumables including 5 mm NMR tubes, deuterated buffers, DMSO and D2O are available at cost from Sue Rhodes.

The Birmingham Structural Biology Forum offers additional facilities for biophysical analysis of proteins including:

  • Atomic Force Microscopy
  • Calorimetry
  • Circular Dichroism
  • Fluorescence
  • FT-ICR mass spectrometry
  • Infrared Spectroscopy
  • Surface Plasmon Resonance
  • X-ray crystallography

We are members of the Technology Hub which provides a range of facilities.

The Protein Expression facility in the School of Cancer Sciences offers:

  • Fermentation (E coli, Pichia) in three refrigerated incubator shakers and two fermentors
  • Protein purification systems: two AKTA Prime and two AKTA Purifier chromatography systems
  • Cell lysis by French Press, Emulsiflex and sonicator
  • UV-VIS spectrophotometer
  • Thermofluor 96 well plate screening for NMR conditions and ligands

The floorplan of the NMR building shows the position of the laboratory

NMR facility floorplan, showing the different areas

 

HWB NMR logo

Photo of spectrometer

The wet lab is conveniently located near the 500 and 600 MHz NMR spectrometers.