The MoFlow Astrios is situated within the CTIR in the QE hospital
It is equipped with five lasers and can detect up to 18 parameters. Only sorter capable of >70,000 sort decisions/second validated performance with a variety of collection options including 6 way sorting and single cell sorting into PCR plates for gene expression analysis.
Capabilities and features-
Sort up to 6 selected populations simultaneously with a variety of temperature controlled (-4 to 40°C) collection options.
Plate sorting up to 1536-well
Mixed Mode sorting: each sort stream is capable of having its own sort mode (Enrich, Purify, Single) programmed. Soft aborts and waste can be collected for reanalysis or resorting.
Dual eFSC channels allow you to apply different application-specific masks for different particle types, or use different neutral density filters to allow a greater overall particle size range to be measurable simultaneously.
Contained within an NSF-49 tested Class II Biosafety Cabinet for greater sterility and biosafety.
lasers- 355, 405, 488, 561 and 642nm.
18 analysis channels- Forward scatter, side scatter,
3 fluorescence channels activated by 355nm laser
3 fluorescence channels activated by 405nm laser
6 fluorescence channels activated by 488nm laser
3 fluorescence channels activated by 561nm laser
3 fluorescence channels activated by 642nm laser
Suitable fluorophores include DAPI, Pacific Blue, FITC, PE, PE-Cy5, PE-Cy7, PE-Texas Red, APC and APC-Cy7