KSHV and the DNA damage response.
KSHV has been shown to be responsible Karposi’s Sarcoma as well as a number of rarer conditions. Many viruses are known to activate cellular DNA damage response pathways. This may either be required for viral replication or may be inhibited by the virus, facilitating replication. In this project we are investigating the relationship of KSHV to the damage response in latently infected cells and during lytic infection.
Characterization of hnRNPUL1.
hnRNPUL1 (also known as E1B-AP5) was first isolated as a binding partner for adenovirus E1B55K protein. It has since been shown to have roles in RNA metabolism, transcriptional regulation (of, for example, p53) and the cellular DNA damage response. In particular hnRNPUL1 appears to be involved in homologous recombination through interaction with NBS1. We are now investigating whether the proteins have potential kinase against nucleotide substrates as well as its interaction with other DNA damage pathway components.