We use molecular and cellular techniques to understand the structure, activation and localisation of large transmembrane proteins. We have identified novel activation mechanisms for the cardiovascular calcitonin gene-related peptide (CGRP) receptor and discovered a new trigger for cellular trafficking of the aquaporin (AQP) family of water pores.
We are interested in understanding the docking of ligands and activation of the second largest family of G protein-coupled receptors (GPCRs). We use site-directed mutagenesis to identify key areas of functional importance with focus on the potent vasodilator calcitonin gene-related peptide (CGRP) and its receptor. We compare receptors though ligand binding, cell-surface expression, second messenger activation and agonist-mediated internalisation/desensitisation.
We use similar techniques to understand the sub-cellular relocalisation of the water channel transmembrane proteins (aquaporins; AQPs). This is important for a number of processes in health and disease including kidney function, stroke and trauma.
- Woolley MJ and Conner AC (2013) Comparing the molecular pharmacology of CGRP and adrenomedullin. Curr Protein Pept Sci 14(5):358-74
- Conner AC, Bill RM and Conner MT (2013) An emerging consensus on aquaporin translocation as a regulatory mechanism. Mol Membr Biol 30(1):1-12
- Vohra S, Taddese B, Conner AC, Poyner DR, Hay DL, Barwell J, Reeves PJ, Upton GJ and Reynolds CA (2012) Similarity between class A and class B G-protein-coupled receptors exemplified through calcitonin gene-related peptide receptor modelling and mutagenesis studies. J R Soc Interface 10(79):20120846
- Conner AC, Conner MT, Bland CE, Taylor LH, Brown JE, Parri HR and Bill RM (2012) Rapid aquaporin translocation regulates cellular water flow: mechanism of hypotonicity-induced subcellular localization of aquaporin 1 water channel. J Biol Chem 287(14):11516-25
- Barwell J, Woolley MJ, Wheatley M, Conner AC and Poyner DR (2012) The role of the extracellular loops of the CGRP receptor, a family B GPCR. Biochem Soc Trans 40(2):433-7
For full publication list see:
Dr Alex Conner
Mr Philip Kitchen
Mr Luke Taylor
Mr Mike Woolley