The Structural Biology and Molecular Biophysics Theme benefits from a diverse range of state-of-the-art analytic facilities.
Birmingham's Advanced Mass Spectrometry facility boasts Thermo Scientific LTQ FT Ultra, Thermo Scientific Orbitrap Velos ETD and Thermo Scientific TSQ Vantage mass spectrometers all equipped with nano-LC separation systems, which facilitate protein identification and quantitation, as well as the characterisation of post-translational modifications of proteins metabolomics analysis.
The Birmingham Biophysical Characterisation Facility (BBCF) features a range of equipment for essential biphysical characterisation of proteins and their complexes with ligands, including circular dichroism (CD), Fourier Transform Infrared Spectroscopy (FTIR), Analytical Ultracentrifugation (AUC), Dynamic Light Scattering (DLS), X-ray Crystallography and Isothermal Titration Calorimetry (ITC).
Macromolecular X-Ray Diffraction Facility is supported by a state-of-the-art microfocus rotating anode generator in combination with a 4-circly kappa goniometer and CCD detector. Crystallisation of proteins takes advantage of a Mosquito nano-litre liquid handling robot and an automated microscope system with attached plate hotel and UV imaging capability.
The Structural Biology and Molecular Biophysics theme has close links to Birmingham's Henry Wellcome Centre for Biomolecular NMR, which houses the National 900 MHz NMR facility.