Dr Sue Armstrong Bsc.PhD

Senior Lecturer

School of Biosciences

Dr Sue Armstrong

Contact details

Telephone +44 (0)121 41 46485

Fax +44 (0)121 41 45925

Email s.j.armstrong@bham.ac.uk

School of Biosciences
University of Birmingham
Edgbaston
Birmingham
B15 2TT
UK

About

My interests are focused on genome evolution and meiosis in both model plants and crops. In 1995 I setup a molecular cytogenetics laboratory with my colleague Paul Fransz (now at University of Amsterdam) and with advice from our colleague Gareth Jones. In the last 15 years we have trained many European colleagues and last year we were awarded a European collaborative project to investigate the meiotic pathway in the model plant Arabidopsis and crop species.  

Qualifications

BSc, PhD

Biography

Initially I taught at a local technical college and combined this with working for a PhD on chromosome staining techniques and interspecific hybridisation in the genus Allium by part-time research. Subsequently I researched on mammalian meiosis, before returning to University of Birmingham in 1995.

Teaching

Undergraduate teaching

BIO 265 Genes and genomes from micro-organisms to man: Mutation and DNA repair, human genetics (Module organiser)

BIO 348 Genetic variation in Humans: Chromosome rearrangements; telomeres, evolution of sex chromosomes( Module organiser)

Post graduate teaching

MSc Analytical Genomics: Module leader; Mapping genes and genetic markers

Postgraduate supervision

For a list of possible PhD projects offered by Dr Armstrong

Research

Research Theme within School of Biosciences: Molecular and Cell Biology

Organisation and behaviour of chromosomes in plant meiosis

My research interests are concerned with meiosis, a highly conserved pathway, by which haploid gametes are formed following a double cell-division process. Our laboratory is interested in chromosome behaviour during pairing, recombination and synapsis in plant meiosis. We use molecular cytogenetic and immunolocalisation techniques combined with the molecular resources available for Arabidopsis molecular biology, to investigate aspects of this complex process that continues to be poorly understood.

We (Professor Franklin and I) have recently been awarded a LOLA award to transfer our Arabidopsis knowledge to investigate meiosis in barley and to find ways to manipulate crossover frequency and distribution in this cereal (see Professor Chris Franklin web-page for details). I am also co-ordinator for an EU collaborative project

Currently my research falls into 3 areas:

1. Telomere biology in Arabidopsis We are investigating how homologous telomeres pair during early meiotic prophase. Recent attention has been paid to the bouquet, a nearly universal event during which telomeres move and cluster in early prophase. We are looking at pairing in telomere deficient plants in order to understand the significance of this early step in the meiotic pathway.

2. Genetic and cytogenetic investigation of homoeologous pairing and recombination in B. Napus (AACC). Breeders wishing to introduce new characteristics into the B. Napus (oil seed rape) crop are creating synthetic lines by crossing B. Oleracea with B. Rapa. In natural B. Napus plants pairing control is strict; (A/A and C/C) whereas in synthetics pairing control is less strict (A/C) and can lead to unstable progeny and loss of fertility. We are investigating the nature of this problem. 

3. Evolution of sex chromosomes in the plant genus Silene. I collaborate with DA Filatov (Oxford). Our joint research interests are concerned with evolution and chromosome behaviour in meiosis in the genus Silene. This plant genus provides an opportunity to conduct a comparative cytogenetical study of the relatively early stages of sex chromosome evolution because it contains cytologically distinguishable heteromorphic sex chromosomes that have evolved relatively recently, 10-20 million years ago. We are using the cytogenetics tools we have developed for Arabidopsis to analyse the changes in sex chromosome structure and meiotic behaviour during sex chromosome evolution in Silene.

We are carrying out a high resolution comparative cytogenetic analysis of the X and Y chromosomes in dioecious Silene. In particular, we are investigating (i) How the structure of chromosomes have evolved to form clearly distinguishable heteromorphic X and Y chromosomes. (ii) How these plants cope with heterologous sex chromosomes during meiosis; specifically do they restrict pairing to the pseudoautosomal region (PAR) of the X and Y in plants? (iii) and what mechanisms are involved in suppressing recombination in the non-recombining regions (NRY) of the Silene Y chromosome? Overall, we would like to know if these mechanisms are similar to those arrived at in mammalian systems or alternatively, are we looking at novel approaches to achieve the same outcome.

Other activities

I am currently the second year academic tutor.

Publications

Publications 2000 onwards...

PF Fransz, SJ Armstrong, J Hans de Jong, LD Parnell, C van Drunen, C Dean, P Zabel, T Bisseling, GH Jones. 2000. Integrated cytogenetic map of chromosome arm 45 of A. Thaliana: structural organization of heterochromatic knob and centromere region. Cell 100, 367-376.

SJ Armstrong, FCH Franklin, GH Jones. 2001. Nucleolus-associated telomere clustering precede meiotic chromosome synapsis in Arabidopsis thaliana. Journal of Cell Science 114, 4207-4217.

EC Howell, GC Barker, GH Jones, MJ Kearsey, GJ King, EP Kop, CD Ryder, GR Teakle, JG Vicente, SJ Armstrong. 2002. Integration of the cytogenetic and genetic linkage maps of Brassica oleracea. Genetics 161, 1225-1234.

SJ Armstrong, GH Jones. 2003. Meiotic cytology and chromosome behaviour in wild-type Arabidopsis thaliana. Journal of Experimental Botany 54, 1-10.

R Mercier, S J Armstrong, C Horlow, N P Jackson, CA Makaroff, D Vezon, G Pelletier, G H Jones, FCH Franklin. 2003. The meiotic protein SWI1 is required for axial element formation and recombination initiation in Arabidopsis thaliana. Development 130, 3309-3318.

SJ Armstrong, FCH Franklin, GH Jones. 2003. A meiotic time course for Arabidopsis thaliana. Sexual Plant Reproduction 16, 141-149.

JD Higgins*, SJ Armstrong*, FCH Franklin, GH Jones. 2004. The Arabidopsis MutS homologue At MSH4 functions at an early step in recombination; evidence for two classes of recombination in Arabidopsis. Genes and Development 18, 2557-2570 * joint first authors

EC Howell, SJ Armstrong, GC Barker, GH Jones, GJ King, CD Ryder, MJ Kearsey. 2005. Physical organization of the major duplication Brassica oleracea chromosome 06 revealed through fluorescence in situ hybridisation with Arabidopsis and Brassica probes. Genome 48, 1-11

N Jackson, E Sanchez-Moran, E Buckling, SJ Armstrong, GH Jones, FCH Franklin. 2006. Reduced crossovers and delayed prophase I progression an AtMLH3 deficient Arabidopsis. EMBO Journal, 25, 1315-1323.

C. Kerzendorfer ,J Vignard , A Pedrosa-Harand A, T Siwiec, S Akimcheva, S Jolivet, R Sablowski, SJ Armstrong, D Schweizer, R Mercier, P Schlöegelhofer. 2006. The Arabidopsis thaliana Meiotic Nuclear Division I (MNDI) homologue plays a key role in meiotic homologous recombination and synapsis. Journal of Cell Science, 119, 2486-2496.

J Vignard, T Siwiec, Lchelysheva, N Vrielynch, F Gonord, SJ Armstrong, P Schlögelhofer, R Mercier 2007. The interplay of RecA-related proteins and the MND1-HOP2 complex during meiosis in Arabidopsis thaliana. . Plos Genet 3(10)

SJ Armstrong, D Filatov. 2008. A cytogenetic view of sex chromosome evolution in plants. Cytogenet. Genome Res, 120: 241-246.

RJ Scott, SJ Armstrong, J Doughty, M Spielman. 2008. Double Fertilization in Arabidopsis thaliana Involves a Polyspermy Block on the Egg but Not the Central Cell. Molecular Plant 1-9.

EC Howell, MJ Kearsey, GH Jones, GJ King, SJ Armstrong. 2008. A and C genome and chromosome identification in Brassica napus by sequential FISH and GISH. Genetics published ahead of print Oct 9 1534/genetics108.095895

DA Filatov, EC Howell, C Groutides, SJ Armstrong. 2009. Recent spread of a retrotransposon in the Silene latifolia genome apart from the Y chromosome. Genetics181, 811-817

NR Roberts, K Osman, SJ Armstrong. 2009. Telomere Distribution and Dynamics in Somatic and Meiotic Nuclei of Arabidopsis thaliana. Cytogenet. Genome Res. 124, 193-201

SJ Armstrong, E Sanchez- Moran, FCH Franklin. 2009. Cytological analysis of Arabidopsis thaliana meiotic chromosomes. Methods in Molecular Biology 558, Meiosis Volume 2 Cytological methods.

EC Howell, SJ Armstrong, DA Filatov. 2011. Dynamic gene order on the Silene latifolia Y chromosome. Chromosoma. 120,  287-295

K Osman, JD Higgins, E Sanchez-Moran, SJ Armstrong, FCH Franklin. 2011. Pathways to meiotic recombination in Arabidopsis thaliana.  New phytologist 190, Issue 3, pages 523–544

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