PhD Title: Investigation of recruitment of EJC and ribosome on transcription sites of Drosophila polytene chromosomes
Supervisors: Dr Saverio Brogna
Gene expression in eukaryotes is much more complicated compare to prokaryotes, as in eukaryotes pre-mRNA processing separate by nuclear envelop from other cytoplasmic events. However research from past and recent evidence suggests that there is link between nuclear and cytoplasmic events and exon junction complex (EJC) is the best model to describe this connecting link. EJC mainly deposit 20-24 nucleotides upstream of spliced junction and guide the mRNA through nuclear pore to the cytoplasm, where they either involve in nonsense mediated decay of mRNA or enhances the translation. I am using Drosophila polytene chromosome to study the recruitment of EJC on transcription sites and investigate their selective association with spliced mRNA.
Studied BSc (Hons) in Microbiology from University of Calcutta, Calcutta, India and MSc Biotechnology from Utkal University, Bhubaneswar, and Orissa, India.
1. Fluorescent protein tagging confirms the presence of ribosomal proteins at Drosophila polytene chromosomes.
Rugjee KN, Roy Chaudhury S, Al-Jubran K, Ramanathan P, Matina T, Wen J, Brogna S.
University of Birmingham , School of Biosciences , UK.
2. Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus.
Khalid Al-Jubran, Jikai Wen, Akilu Abdullahi, Subhendu Roy Chaudhury, Min Li, Preethi Ramanathan, Annunziata Matina, Sandip De, Kim Piechocki,Kushal Nivriti Rugjee, Saverio Brogna
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.