Dr Dalan Bailey BSc PhD

Dr Dalan Bailey

Institute of Immunology and Immunotherapy
Birmingham Fellow / Principal Investigator

Contact details

Address
Institute of Immunology and Immunotherapy
Centre for Human Virology
University of Birmingham
Edgbaston
Birmingham
B15 2TT
UK

Dr. Dalan Bailey is a University of Birmingham Research Fellow working on the molecular biology of RNA viruses including measles, respiratory syncytial virus and more exotic pathogens such as Ebola and Nipah virus.

Dalan is particularly interested in the virus-host interactions that take place during virus entry and exit. His focus lies in characterising the interplay between these viral processes, the cytoskeleton and innate immunity. Since 2005 he has published over 25 manuscripts in this area including publications in PLOS Pathogens, JBC and the Journal of Virology.

Dalan is actively involved in research, lecturing and the supervision of junior researchers and in the last 3 years has supervised over 10 undergraduate and postgraduate students in his lab. Dalan is looking to expand his laboratory and is interested to hear from prospective PhD students with international scholarships who are looking for a supportive research environment for their research studies.

Qualifications

  • PhD Virology 2007
  • BSc Virology (Hons) 2002

Biography

Dalan graduated from the University of Warwick in 2002 with a BSc Honours degree in Virology. To further his career in virology he undertook a PhD in the lab of Prof. Tom Barrett at the Pirbright Institute (formerly the Institute for Animal Health) where he worked on morbilliviruses. He was the first person to sequence the genome of Peste-des-petits-ruminants virus (PPRV), a close relative of Measles virus (work published in his first paper in 2005 http://www.ncbi.nlm.nih.gov/pubmed/15845262). Dalan’s work at the institute focused on the development of molecular biology tools, including reverse genetics systems, to dissect the molecular determinants of pathogenesis for morbilliviruses. He was awarded his PhD from the University of Reading in 2007 and soon after began working as a postdoctoral researcher. 

During his five year postdoctoral research contract (at Imperial College – London) Dalan worked on noroviruses, small positive sense RNA viruses in the Caliciviridae family. While in the lab of Prof. Ian Goodfellow, Dalan further developed his research into the areas of proteomics and protein-protein interactions while maintaining a keen interest in pathogenesis. The main focus of his work during this time was the murine norovirus pathogen, an excellent surrogate model for the human virus and disease. This virus was identified in 2003 and together with colleagues at Imperial Dalan published research on this virus in many leading virology journals. 

This research focused on manipulation of a reverse genetics model for murine norovirus and subsequent analysis of the molecular biology of viral replication and disease. During this time as a postdoctoral researcher Dalan began co-supervising PhD and MSs students in the lab and also became more involved in peer-reviewing academic manuscripts. 

In 2013 Dalan moved to the University of Birmingham to take up a fellowship role in the School of Immunity and Infection. His major area of research at the University is on the negative sense RNA viruses, especially the paramyxoviruses – a broad group of pathogens that infect multiple species and cause a great health and economic burden across the globe. 

Teaching

  • Lecturer in BMedSci course: virology
  • External lecturer at: LSHTM, Surrey, Imperial College universities (on-going).

Postgraduate supervision

Dalan currently supervises two PhD students, from Portugal and Malaysia.

He is interested in supervising doctoral research students in the following areas:

  • The molecular virology of paramyxoviruses (e.g. Measles, RSV, hPIV3, Mumps, Hendra)
  • Enveloped virus attachment and entry
  • Viral budding and release
  • Innate immunity

If you are interesting in studying any of these subject areas please contact Dalan at the email address listed above

Research

PARAMYXOVIRUSES 

The Paramyxovirus family of viruses encompasses two sub-families and seven genera. Classified viruses within this family include the human pathogens measles, mumps, respiratory syncytial virus and human parainfluenza. Pathogens of animals are also significantly represented including rinderpest (now eradicated), peste des petits ruminants and Newcastle disease. There are also important emerging zoonotic viruses in this family including Hendra and Nipah. Collectively these viruses are a great health and economic burden across the globe resulting in numerous deaths and considerable hardship, yet there is a lack of effective vaccines and antivirals. 

Paramyxoviruses all have non-segmented negative sense RNA genomes and are enveloped. This plasma membrane is itself stolen from the host cell during the process of virion release (budding). Embedded in the membrane of these viruses are virally encoded proteins that permit the attachment of the virus to uninfected cells and the subsequent entry of the viral genome. 

RESEARCH ACTIVITY 

The core aims of the Bailey laboratory are to improve understanding of paramyxovirus virology and basic cell biology and highlight the integral role of proteomics and systems biology in modern virology. Systematic analysis of the paramyxovirus family will improve knowledge of virus-host interactions, host restriction, pathogenesis and host range. This basic research will then be applied to the development of novel antivirals and vaccines. 

Using novel proteomics techniques, as well as established molecular virology tools, the laboratory focuses on the following aspects of paramyxovirus biology: 

Virus-host interactions: 

Like all viruses the paramyxoviruses rely on hijacking cellular machinery in order to replicate and spread. Identifying the protein-protein and RNA-protein interactions underpinning this paradigm is an important focus of the laboratory. The Bailey laboratory has previously identified the epithelial receptor of a ruminant paramyxovirus (PPRV), an important step in understanding the molecular determinants of pathogenesis for this virus. 

Attachment and entry: 

The interaction between virus particles (virions) and their hosts (cells) is often determined by the specific affinities of viral attachment proteins for host receptors. The strength and specificity of these interactions plays an important role in determining the host range and cell-specificity (tropism) of viruses. This, in turn, has an important effect on the nature and severity of disease caused by the virus. Determining the restrictions and mechanism involved in these virus-host interactions is an on-going area of research in the Bailey laboratory. 

Budding and release: 

Paramyxoviruses have evolved two distinct strategies to spread from infected to uninfected cells. The classical strategy is via the formation and release of enveloped infectious particles while the other employs cell to cell fusion, allowing spread without virion production. Understanding the mechanisms behind these two processes is a key goal of the research in the Bailey laboratory.

Cytoskeleton and innate immunity:

The Bailey laboratory is also interested in examining the links between viral entry/exit and the innate immune response. We are particularly interested in how the cytoskeleton may provide the basis for this response.

Other activities

  • Member of the Society for General Microbiology (2004-present)
  • Member of the Veterinary Research Club (2008-present)
  • Advisory board member for Archives of Virology (2010-present)

Publications

  1. Identifying novel protein interactions: Proteomic methods, optimisation approaches and data analysis pipelines. Carneiro DG, Clarke T, Davies CC, Bailey D. Methods. 2015 Aug 29. http://www.ncbi.nlm.nih.gov/pubmed/26320829
  2. Serological evidence of camel exposure to peste des petits ruminants virus (PPRV) in Nigeria. Woma TY, Kalla DJ, Ekong PS, Ularamu HG, Chollom SC, Lamurde II, Bajehson DB, Tom ND, Aaron GB, Shamaki D, Bailey D, Diallo A, Quan M. Trop Anim Health Prod. 2015 Mar;47(3):603-6. http://www.ncbi.nlm.nih.gov/pubmed/25547805
  3. The murine norovirus (MNV) core subgenomic RNA promoter consists of a stable stem-loop that can direct accurate initiation of RNA synthesis. Yunus MA, Lin X, Bailey D, Karakasiliotis I, Chaudhry Y, Vashist S, Zhang G, Thorne L, Kao CC, Goodfellow I. J Virol. 2014 Nov 12. http://www.ncbi.nlm.nih.gov/pubmed/25392209
  4. Detection of protein-protein interactions using tandem affinity purification. Methods Mol Biol. Goodfellow I and Bailey D. 2014;1177:121-33. http://www.ncbi.nlm.nih.gov/pubmed/24943319
  5. Norovirus translation requires an interaction between the C Terminus of the genome-linked viral protein VPg and eukaryotic translation initiation factor 4G. Chung L, Bailey D, Leen E, Emmott E, Chaudhry Y, Roberts L, Curry S, Locker N, Goodfellow IG. J Biol Chem. 2014 Aug 1;289(31):21738-50. http://www.ncbi.nlm.nih.gov/pubmed/24928504
  6. Pathology caused by persistent murine norovirus infection. Shortland A, Chettle J, Archer J, Wood K, Bailey D, Goodfellow IG, Blacklaws BA, Heeney JL. Journal of General Virology. 2013 Nov 13. http://www.ncbi.nlm.nih.gov/pubmed/24225497.
  7. McFadden N, Arias A, Dry I, Bailey D, Witteveldt J, Evans DJ, Goodfellow I, Simmonds P. Influence of genome-scale RNA structure disruption on the replication of murine norovirus--similar replication kinetics in cell culture but attenuation of viral fitness in vivo. Nucleic Acids Research. 2013 Apr 29. http://www.ncbi.nlm.nih.gov/pubmed/23630317.
  8. Pope RA, Parida S, Bailey D, Brownlie J, Barrett T, Banyard AC. Early events following experimental infection with Peste-Des-Petits ruminants virus suggest immune cell targeting. PLoS One. 2013;8(2):e55830. http://www.ncbi.nlm.nih.gov/pubmed/23418464.
  9. Birch J, Juleff N, Heaton MP, Kalbfleisch T, Kijas J and Bailey D. Characterization of ovine nectin-4, a novel peste des petits ruminants virus receptor. http://www.ncbi.nlm.nih.gov/pubmed/23388720. Journal of Virology. 2013 Apr;87(8):4756-61.
  10. Thorne L, Bailey D and Goodfellow I (2012). High-Resolution functional profiling of the murine norovirus genome. Journal of Virology. 2012 Nov;86(21):11441-56. http://www.ncbi.nlm.nih.gov/pubmed/22915807.
  11. Arias A, Bailey D, Chaudhry Y and Goodfellow I (2012).Development of a reverse genetics system for murine norovirus 3; long-term persistence occurs in the caecum and colon. Journal of General Virology 93(Pt 7):1432-41. http://www.ncbi.nlm.nih.gov/pubmed/22495235
  12. Buczkowski H, Parida S, Bailey D, Barrett T and Banyard AC (2012). A novel approach to generating morbillivirus vaccines: Negatively marking the rinderpest vaccine. Vaccine 30(11):1927-35. http://www.ncbi.nlm.nih.gov/pubmed/22265946
  13. Bailey D, Urena L and Goodfellow I (2012). Identification of protein interacting partners using tandem affinity purification. Journal of Visualized Experiments 25(60).  http://www.ncbi.nlm.nih.gov/pubmed/22395237
  14. McFadden N*, Bailey D* (joint first authors), Carrara G, Benson A, Chaudhry Y, Shortland A, Heeney J, Sosnovtsev S, Yarovinsky F, Simmonds P, Macdonald A and Goodfellow I (2011).Norovirus regulation of the innate immune response and apoptosis occurs via the product of the alternative open reading frame 4. PLOS Pathogens 7(12):e1002413.http://www.ncbi.nlm.nih.gov/pubmed/22174679
  15. Yunus A, Chung L, Chaudhry Y, Bailey D, Goodfellow IG (2010). Development of an optimized RNA-based murine norovirus reverse genetics system. Journal of Virological Methods 169(1):112-8. http://www.ncbi.nlm.nih.gov/pubmed/20637238
  16. Putics A, Vashist S, Bailey D, Goodfellow IG (2010). Chapter 11: Murine norovirus translation, replication and reverse genetics. Book: Caliciviruses: Molecular and Cellular Virology (Caister Academic Press). http://www.horizonpress.com/calicivirus
  17. Karakasiliotis I, Vashist S, Bailey D, Abente EJ, Green KY, Roberts LO, Sosnovtsev SV, Goodfellow IG (2010). Polypyrimidine tract binding protein functions as a negative regulator of feline calicivirus translation. PLoS One 10;5(3):e9562. http://www.ncbi.nlm.nih.gov/pubmed/20224775
  18. Bailey D*, Karakasiliotis I, Vashist S, Chung LM, Reese J, McFadden N, Benson A, Yarovinsky F, Simmonds P, Goodfellow IG (2010).  Functional analysis of RNA structures present at the 3' extremity of the murine norovirus genome: the variable polypyrimidine tract plays a role in viral virulence. Journal of Virology 84(6):2859-70. * highlighted by Journal of Virology in their ‘Spotlights’ section as a research article of significant interest. http://www.ncbi.nlm.nih.gov/pubmed/20053745
  19. Bailey D, Kaiser WJ, Hollinshead M, Moffat K, Chaudhry Y, Wileman T, Sosnovtsev SV, Goodfellow IG. (2010) Feline calicivirus p32, p39 and p30 proteins localize to the endoplasmic reticulum to initiate replication complex formation. Journal of General Virology 91(Pt 3):739-49. http://www.ncbi.nlm.nih.gov/pubmed/19906938