Primate cerebellum showing intense staining of neuronal nuclei (arrows) in the molecular layer (ML). There is no substantial staining of the Purkinje cells (PC) or granular layer (GL) neurones.
At a higher magnification, generalised speckled staining of the molecular layer appears similar to that seen with amphiphysin, The neuronal nuclei (NN) give the impression of being masked with the speckled staining.
On the Western blot there were no bands seen. This sample had homogenous pattern on the HEp-2 cells. Anti-nuclear antibody usually cross reacts with all the neuronal nuclei.
This is a 67 year old female with peripheral neuropathy and her serum has shown a similar pattern over the last three years.