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PRODID:-//University of Birmingham//Events//EN
VERSION:2.0
CALSCALE:GREGORIAN
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DTSTAMP:20240109T105000Z
DTSTART:20240513T081500Z
DTEND:20240516T150000Z
SUMMARY:LC-MS based metabolomics and metabolite identification with the Q Exactive™ Plus and Orbitrap ID-X™ Tribrid™
UID:www.birmingham.ac.uk/206960
DESCRIPTION:


 

LC-MS based metabolomics and metabolite identification with the Q Exactive™ Plus and Orbitrap ID-X™ Tribrid™
 13th - 16th May 2024 | Course now full (email bmtc@contacts.bham.ac.uk for waiting list access)\n \n





Course Overview
 This 4-day course introduces LC-MS based metabolomics and metabolite identification using both the Q Exactive Plus (QE+) and Orbitrap ID-X Tribrid mass spectrometers, guiding delegates through experimental design, sample preparation, using the QE+ and ID-X, data processing, univariate and multivariate data analysis, and metabolite annotation/identification. It is an intensive course designed to provide foundational knowledge for delegates to develop further skills and undertake their own metabolomics experiments. \n
 This course is led and delivered by five experts in the field of metabolomics and includes lectures, hands-on laboratory sessions, and computer workshops to provide a detailed overview of the metabolomics pipeline.\n







Topics Covered

Introduction to QE+ and ID-X mass spectrometers and the metabolomics workflow
Experimental design and the importance of quality control (QC) samples
Sample preparation including extraction of polar metabolites and lipids from biofluids (urine and plasma) and tissue samples
Hands-on data acquisition for untargeted metabolomics and hybrid metabolomics, the latter combining targeted and untargeted measurements
Setting up the Vanquish UHPLC coupled to the QE+
Types of data which can be collected on the QE+ and and ID-X (m/z, retention time, MS/MS, MSn)
MS/MS and MSn experiments
Data processing workshop
Data analysis workshop (univariate and multivariate analysis)
Introduction to metabolite identification applying Data Dependent Analysis
Conversion of raw data to molecular formula and putative metabolite annotations
Mass spectral libraries (using mzCloud), and how to search them
Tools for mass spectral interpretation
Reporting standards for metabolite identification
Question and answer session with a panel of experts

 Preview the full course programme here\n



 

Level 
 The course is aimed at individuals with minimal previous experience of applying LC-MS in metabolomics studies. A basic working knowledge of LC-MS would be highly advantageous.\n




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Early-bird: £1,349
 Course now full (email bmtc@contacts.bham.ac.uk for waiting list access)\n \n




 \n


Standard: £1,810
 Course now full (email bmtc@contacts.bham.ac.uk for waiting list access)\n \n







MRC Bursary: £0

Status: CLOSED\n 
Application deadline: CLOSED
See our Trainee Information Page here for more information, terms and conditions.

CLOSED



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 \n Please read the Birmingham Metabolomics Training Centre's Conditions and Cancellation Policy prior to registering.\n




LOCATION:School of Biosciences - University of Birmingham
STATUS:CONFIRMED
TRANSP:OPAQUE
CLASS:PUBLIC
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