The following services are offered by the NERC Metabolomics Facility.
Before you apply to use the facility, we will discuss with you how best to design your metabolomics experiment.
B. Sample Extraction
Extraction of metabolites from biological matrices.
This will be routinely carried out by visiting scientists after training provided by us. Each sample will be either extracted to provide a polar and non-polar fraction, based on the method by Bligh and Dyer, or by monophasic extraction.
C. Mass Spectrometry
We currently offer routinely:
- Non-targeted nanoelectrospray direct infusion MS (DIMS) of polar and lipid metabolites in positive and negative ion modes.
- Non-targeted LC-MS of polar and lipid metabolites in positive and negative ion modes.
D. Nuclear Magnetic Resonance Spectroscopy
We currently offer 1-D and 2-D J-resolved NMR spectroscopy of polar metabolites on a Bruker 600 MHz spectrometer.
E. Data Analysis
Raw spectral data will be processed using mostly custom-written software. Subsequent data analysis will comprise of univariate and/or multivariate methods, and an initial output will be assembled from the statistical results and MS & NMR signal annotations (e.g. MIPack or Chenomx results).
Visitors are welcome conduct their own statistical analyses, after training by NBAF-B staff. Our goal is to enable publication of the results.