Research activities

Seahorse XF analyzers

The Seahorse XF analysers within the MPC have been used to support a wide range of research projects by researchers at the University of Birmingham (see Publications).

Ongoing research

Professor Gareth Lavery

The Lavery lab are currently using XF technology to explore the role of Nicotinamide Riboside Kinase 1 on mitochondrial function in pancreatic islets. This is to support their ongoing investigation of NAD+ related therapies.

Dr Debbie Cunningham

Dr Cunningham’s lab are using XF analysis to identify the effect of tyrosine phosphatase on the regulation of cellular metabolism.

“The MPC has assisted my PhD research project which involves studying a novel metabolic phenotype we have observed in knockout cells. The facility has been incredibly supportive; providing the facilities, equipment and most importantly the expertise and knowledge needed to investigate this phenotype." 
-  Miss Carys Howell (PhD student, Dr Cunningham’s Lab) 

Oroboros O2K FluoRespirometry

Ororboros O2K FluoRespirometers within the MPC are currently used to support a range of internal and external research projects throughout the West Midlands. Specifically, the MPC supports research carried out by Professor Philip Atherton from the University of Nottingham, and Dr. Leigh Breen from the University of Birmingham who utilise HRR using the Oroboros O2K to investigate the demise and protective effects of exercise on mitochondrial function in isolated human skeletal muscle fibres during ageing and disease.

Dr Yun Fan from the University of Birmingham is currently using Oroboros O2K technology within the MPC to explore the function of mitochondria in mechanisms related to cell death and the control of tissue growth using Drosophila as an experimental model.

Future technologies

Dr Jonathan Barlow, the manager of the MPC is currently investigating a way to explore mitophagy in live cells in ‘real-time’ using Mito QC. The aim of this project is to use Mito QC as a way to investigate mitophagy in ‘real-time’ and to use this technology to produce Mito QC cell lines and/or transgenic mouse models for users interested in exploring mitochondrial morphology as well as function. The MPC believe that this will be a unique opportunity to offer a service to explore mitochondrial morphology and mitochondrial function using the same cells and tissues.