Bioseparations
Module Title - Bioseparations
Number of credits – 20
The following topics are covered in the module:
Bioseparations 1
- Introduction to Downstream Processing (DSP)
- Developing DSP strategies for rDNA products
- Cell disruption strategies for intracellular products
- Aggregation mechanisms and processes
- Fractional precipitation
- Centrifugation and industrial centrifuges
- Conventional filtration and membrane separations
- Industrial case study (Novo-Nordisk): Manufacture of human growth hormone HGH
Bioseparations 2
- Introduction to process chromatography
- Understanding the factors that affect resolution & scale-up in chromatography
- Adsorption-desorption processes underpinning chromatography
- Modelling protein adsorption
- Adiabatic compressibility of proteins
- The three phase strategies & how to link steps
- Protein purification – the right step at the right time
- Chromatographic process development
- Industrial case study (Millipore): Process optimisation & intensification in scale-up of chromatography
- Development of chromatographic media
- New media designs, new separation formats and alternatives to chromatography
- Convective interactive monolith media
- Expanded bed adsorption
- High-gradient magnetic fishing
- The ideal magnetic support
- Aqueous Two-Phase Extraction