Bioseparations

Module Title - Bioseparations
Number of credits – 20

Module description

The first half of this module (Bioseparations 1) introduces students to the theories and principles of general downstream processing, the choice of recombinant protein hosts, cell disruption strategies, aggregation, fractional precipitation, centrifugation and centrifuges, conventional filtration and membrane separations.

The second half of the module (Bioseparations 2) covers high-resolution separations with strong emphasis on process chromatography (overview of the chromatographic process, the major separation principles employed and media involved; adsorption and mass transport processes underpinning chromatography; factors that affect resolution in chromatography; how to design a chromatographic process train – the three phase strategy, linking of chromatographic operations and ‘the right step at the right time’; chromatographic process development, optimization and intensification; current and future support designs; how to characterise adsorbent performance – modelling, adiabatic compressibility of proteins, case studies) and newer competing techniques (Expanded Bed Adsorption, High-Gradient Magnetic Fishing, Aqueous Two Phase Extraction).  

By the end of the module you will be able to:

  • Possess a comprehensive knowledge of the practices employed in the recovery of industrially important molecules typically produced by fermentation.
  • Systematically design detailed downstream process design flow sheets for any bioproduct when supplied with relatively little information concerning, e.g. the intended use, amount required, the host, and some characteristics of the product.
  • Select the appropriate equipment, chromatographic materials and process chemicals for each of the various stages in a downstream process.

Teaching and assessment:

  • Semesters 1 and 2.
  • Approximately around 60 hours contact time.
  • 100% written exam (June).
  • Reassessment: 100% exam (August).