Developing methods to image non-flourescent anticancer cylinders in cancer cell lines

Project completed 2012. 

Supervisors:
Professor Mike Hannon, School of Chemistry
Dr Nik Hodges, School of Biosciences
Professor Ela Claridge, School of Computer Science

This aims of this project are as follows:

- To image the effects of anti-cancer cylinders on cell morphology and motility, and probe the causes of these responses at the molecular level to better understand biological activity.

- To develop a highly fluorescent cylinder as a new molecular probe by conjugating with organic fluorophores.

- To image the uptake, localization and trafficking of these agents.

- To image the localization of these agents in cancer cell lines, excised primary cancer cells and tissues.

- To image the effects of treatment on different cancer cell types.

The project will focus on a new class of compounds (metal-containing supramolecular cylinders) which bind to Y-shaped junctions in DNA and also show exciting activity against cancers in cell line studies. New cylinders with fluorescent tags will be prepared and investigations of their fluorescence and biomolecule binding properties will be undertaken. Fluorescence microscopy will be used to monitor the cell uptake and localisation, and different fluorescent probes will be added to light up particular organelles in the cells, thus confirming cylinder location.

Link to ethesis: http://etheses.bham.ac.uk/5503/