Imaging of platelet actin dynamics with high spatial and temporal resolution

Project completed 2012. 

Supervisors:
Dr Steve Thomas, Institute of Cardiovascular Sciences
Professor Zoe Pikramenou, School of Chemistry
Professor Ela Claridge, School of Computer Science

This project aims to study the dynamics of human platelet actin structures, in particular focusing on the actin nodule. Delivery will focus upon novel optical probes, including Lifeact peptide and luminescent gold nanoparticles. This will enable imaging of actin nodule dynamics with high spatial and temporal resolution using a variety of microscopic techniques, including epifluorescence and confocal fluorescence microscopies, Total Internal Reflection Fluorescence (TIRF) microscopy and electron microscopy.

Link to ethesis: http://etheses.bham.ac.uk/5173/