Liquid micro-junction surface sampling and MALDI imaging of small and large molecules in human liver disease

Project completed 2014.

Supervisors:
Dr Josephine Bunch, School of Chemistry
Dr Hamid Dehghani, School of Computer Science
Dr Patricia Lalor, Institute of Immunology and Immunotherapy
Dr Iain Styles, School of Computer Science
Dr Ata Kaban, School of Computer Science

The major focus of this project will be the development of LESA as an imaging method. A comparison between desorption ionisation (MALDI) and electrospray ionisation (LESA) could then be made. This work explores the use of both MALDI Imaging (with N2 and Nd:YVO4 lasers) and liquid microjunction (LMJ) surface sampling analysis. This would involve optimising methods for both approaches to profile and image lipids and proteins in liver samples. Liver sections for analysis will be obtained from a depository of ethically approved human biopsies will include samples from patients with normal liver, fatty liver, non-alcoholic steatohepatitis, alcoholic liver disease and primary billary cirrhosis. By using livers from this source advancements can be made to further the understanding of the progression of liver disease.

Link to ethesis: http://etheses.bham.ac.uk/6609/