Nanoparticle delivery of nucleic acid probes for SNP detection in the cell and sIRNA mediated gene inactivation

Project completed 2015.

Professor James Tucker, School of Chemistry
Professor Zoe Pikramenou, School of Chemistry
Dr Iain Styles, School of Computer Science
Professor Phil Newsome, Institute of Immunology and Immunotherapy

Project aims:

1. To bind SNP sensing probes to gold nanoparticles for greater emission sensitivity and to allow the delivery of SNP sensing probes into cells without the need for chemical additives.

2. To bind a novel luminescent probe to gold nanoparticles alongside the SNP sensing probe. The additional luminescent probe will act as a standard to which the fluorescent emission variations of the SNP sensing probe can be compared.

3. To bind siRNA to gold nanoparticles silencing a novel tumour target. Binding a novel luminescent probe to gold nanoparticles alongside siRNA will enable the siRNA-AuNP conjugate to be tracked in vivo.