Annalies Muscat obtained the Bachelor of Laws (LLB) in 2007 and the Doctor of Laws (LLD) in 2010, both from the University of Malta. From 2008 to 2010 she was a legal intern with a commercial law firm in Malta. In 2010 she was also a research assistant within the Department of Public Laws at the University of Malta. Annalies continued her studies at the University of Cambridge, where she was awarded the Master of Law (LLM) degree in 2011 with a First Class classification. The same year she was also admitted to the Maltese bar.
After completing her studies, Annalies joined Mamo TCV Advocates in September 2011 as an associate, where she practices commercial law, in particular competition law. Annalies was promoted to senior associate in January 2016. She is also a casual lecturer (teaching associate) at the Faculty of Laws within the University of Malta, where she lectures in competition and consumer law.