Dr Neville Gilhooly

Dr Neville Gilhooly

Institute of Cancer and Genomic Sciences
Marie Skłodowska-Curie Fellow

Contact details

Telephone
+44 (0)121 414 4180
Email
ngilhooly@bham.ac.uk
Twitter
@GilhoolyNev
View my research portal
Address
Room WX2.62, IBR West
Institute of Cancer and Genomic Sciences
University of Birmingham
Edgbaston
Birmingham
B15 2TT
UK

Dr Neville Gilhooly is a Marie Skłodowska-Curie Fellow working in Dr Aga Gambus’ laboratory. His research focuses on understanding the regulatory processes and molecular details that underpin the termination phase of DNA replication. He approaches these problems using a combination of biochemical and biophysical techniques, notably real time single-molecule fluorescence imaging. He has a long-standing interest in the fields of DNA replication and repair and determining how these pathways are coordinated with one another at the molecular level.

Qualifications

2019 - Marie Skłodowska-Curie Fellowship, University of Birmingham

2016 - AACR Anna D. Barker Fellowship in Basic Cancer Research, University of California, Davis

2013 – PhD in Biochemistry, University of Bristol

2009 – Biochemistry BSc, University of Bristol

Biography

Dr Gilhooly obtained his BSc in Biochemistry from the University of Bristol in 2009. He remained in Bristol for his PhD under the supervision of Dr Mark Dillingham working on the Mechanism of Recombination Hotspot Recognition by an AddAB-type helicase-nuclease. In 2014, he moved to the University of California, Davis to pursue postdoctoral work with Professor Stephen Kowalczykowski on single-molecule visualisation of bacterial DNA replication, in collaboration with Dr Ken Marians (MSKCC). He was awarded an Anna D Barker fellowship during this period. In 2019, Dr Gilhooly returned to the UK on a Marie Curie fellowship to undertake work in Aga Gambus’ laboratory on replication termination. He has a broad interest in genome duplication and maintenance, utilising single-molecule biophysics and biochemical approaches to study these processes.

Publications

Gilhooly, N.S., Carrasco, C., Gollnick, B., Wigley, D.B., Moreno-Herrero, F. and Dillingham, M.S. (2016) Chi hotspots trigger a conformational change in the helicase-like domain of AddAB to activate homologous recombination. Nucleic Acids Res. 2016 Apr 7;44(6):2727-41. doi: 10.1093/nar/gkv1543.

Saikrishnan, K.,* Yeeles, J.T.,* Gilhooly, N.S.,* Krajewski, W.W.,* Dillingham, M.S. and Wigley, D.B. (2012) Insights into Chi recognition from the structure of an AddAB-type helicase-nuclease complex. EMBO J, 31, 1568-1578. (* co-first author).

Gilhooly, N.S. and Dillingham, M.S. (2014) Recombination hotspots attenuate the coupled ATPase and translocase activities of an AddAB-type helicase-nuclease. Nucleic Acids Res. 2014 May 1;42(9):5633-43. doi: 10.1093/nar/gku188.

Gollnick, B., Carrasco, C., Zuttion, F., Gilhooly, N. S., Dillingham, M. S. & Moreno-Herrero, F. (2014). Probing DNA Helicase Kinetics with Temperature-Controlled Magnetic Tweezers. Small. 2015 Mar 18;11(11):1273-84. doi: 10.1002/smll.201402686. Epub 2014 Nov 14.

Carrasco, C., Gilhooly, N.S., Dillingham, M.S. and Moreno-Herrero, F. (2013) On the mechanism of recombination hotspot scanning during double-stranded DNA break resection. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):E2562-71.

Green, M., Gilhooly, N.S., Shahriar, A., Scott, D.J., Dillingham, M.S. and Soultanas, P. (2014) Engineering a Reagentless Biosensor for Single-Stranded DNA to Measure Real-Time Helicase Activity in Bacillus. Biosens Bioelectron. 2014 Nov 15;61:579-86. doi: 10.1016/j.bios.2014.06.011. Epub 2014 Jun 11.

Gilhooly, N.S., Gwynn E.J., Dillingham, M.S. (2013) Superfamily 1 helicases. Frontiers in Bioscience S5, 206-216 (Review).

View all publications in research portal