Dr Gilhooly obtained his BsC in Biochemistry from the University of Bristol in 2009. He remained in Bristol for his PhD under the supervision of Dr Mark Dillingham working on the Mechanism of Recombination Hotspot Recognition by an AddAB-type helicase-nuclease. In 2014, he moved to the University of California, Davis to pursue postdoctoral work with Professor Stephen Kowalczykowski on single-molecule visualisation of bacterial DNA replication, in collaboration with Dr Ken Marians (MSKCC). He was awarded an Anna D Barker fellowship during this period. In 2019, Dr Gilhooly returned to the UK on a Marie Curie fellowship to undertake work in Aga Gambus’ laboratory on replication termination, utilising his nascent expertise in single-molecule imaging techniques.