Dr Abdullah O Khan BSc (Hons) MRes PhD.

Dr Abdullah Khan

Institute of Cardiovascular Sciences
BHF Post-Doctoral Fellow

Contact details

Address
College of Medical and Dental Sciences
Medical School
University of Birmingham
Edgbaston
Birmingham
B15 2TT
UK

Dr. Khan is a BHF Post-Doctoral researcher in the Birmingham Platelet Group working on using induced pluripotent stem cells (iPSC), CRISPR genome editing, and super-resolution microscopy to investigate inherited bleeding disorders. Dr. Khan’s research interests also include designing and engineering 3D culture environments for the study of megakaryocyte biology.

Qualifications

  • PhD in Clinical and Experimental Medicine (2019)
  • MRes in Molecular Mechanistic Toxicology (2013)
  • BSc (Hones) in Human Biology (2010)

Biography

Dr. Khan completed his PhD in 2019 before beginning a position as a British Heart Foundation funded post-doctoral researcher in the Birmingham Platelet Group. Dr. Khan’s PhD focussed on developing methods for the genomic expression of single molecule imaging reporters, and the differentiation of induced pluripotent stem cells (iPSCs) into the bone marrow resident progenitors of blood platelets, megakaryocytes. Over the course of his 3 year PhD, Dr. Khan has produced 4 first author papers and co-authored a further 6. He has been awarded a number of internal grants, including a Proximity to Discovery Award and a BHF Accelerator Disruptive Idea Award. He has presented extensively at conferences nationally and internationally, and won a number of poster prizes.

Dr. Khan’s current and future research directions are focussed on using 3D bioprinting to engineer physiologically relevant environments for the study of megakaryocyte biology. He is interested in developing these approaches alongside cutting edge lightsheet imaging techniques at the COMPARE facility to generate 3D live cell imaging approaches for the development of novel therapies.

Research

Dr. Khan’s current research focuses on dissecting the mechanisms by which post-translational modifications of tubulin residues drive the production and function of platelets. His work involves using stem cell and genome editing models, as well as advanced light microscopy to interrogate the effects of specific families of mutations on megakaryocyte and platelet biology.

Dr. Khan’s research interests also include developing 3D culture models to better emulate the complex physiological
environment in which megakaryocytes develop. He aims to use 3D bioprinting in collaboration with Dr. David Bassett
and Dr. Gowsihan Poolagasundarampiallai to establish new complex culture systems.

Other activities

Dr. Khan is a member of the West Midlands Paraclimbing Club (WMPC).

Publications

Recent publications

Article

Almazni, IAF, Stapley, R, Khan, A & Morgan, N 2020, 'A comprehensive bioinformatic analysis of 126 patients with an inherited platelet disorder to identify both sequence and copy number genetic variants', Human Mutation, vol. 41, no. 11, pp. 1848-1865. https://doi.org/10.1002/humu.24114

Pike, J, Simms, V, Smith, C, Morgan, N, Khan, A, Poulter, N, Styles, I & Thomas, S 2020, 'An adaptable analysis workflow for characterization of platelet spreading and morphology', Platelets. https://doi.org/10.1080/09537104.2020.1748588 Abstract

Khan, A, Maclachlan, A, Lowe, G, Nicolson, P, Al Ghaithi, RHR, Thomas, S, Watson, S, Pike, J & Morgan, N 2020, 'High-throughput platelet spreading analysis: a tool for the diagnosis of platelet-based bleeding disorders', Haematologica, vol. 105, pp. e124-e128. https://doi.org/10.3324/haematol.2019.225912

Nicolson, P, Nock, SH, Hinds, J, Garcia Quintanilla, L, Smith, C, Campos, J, Brill, A, Pike, J, Khan, A, Poulter, N, Kavanagh, D, Watson, S, Watson, CN, Clifford, H, Huissoon, A, Pollitt, A, Eble, JA, Pratt, D, Watson, S & Hughes, C 2020, 'Low dose Btk inhibitors selectively block platelet activation by CLEC-2', Haematologica. https://doi.org/10.3324/haematol.2019.218545

Khan, A, Stapley, R, Pike, J, Wijesinghe, S, Reyat, J, Almazni, IAF, Machlus, K & Morgan, N 2020, 'Novel gene variants in patients with platelet-based bleeding using combined exome sequencing and RNAseq murine expression data: Novel genes in patients with platelet-based bleeding', Journal of Thrombosis and Haemostasis. https://doi.org/10.1111/jth.15119

Khan, AO, Maio, AD, Guggenheim, EJ, Chetwynd, AJ, Pencross, D, Tang, S, Belinga-Desaunay, M-FA, Thomas, SG, Rappoport, JZ & Lynch, I 2020, 'Surface chemistry-dependent evolution of the nanomaterial corona on TiO2 nanomaterials following uptake and sub-cellular localization', Nanomaterials, vol. 10, no. 3, 401. https://doi.org/10.3390/nano10030401

Pike, J, Khan, A, Pallini, C, Thomas, S, Mund, M, Ries, J, Poulter, N & Styles, I 2020, 'Topological data analysis quantifies biological nano-structure from single molecule localization microscopy', Bioinformatics, vol. 36, no. 5, pp. 1614–1621. https://doi.org/10.1093/bioinformatics/btz788

Khan, A, White, CW, Pike, J, Yule, J, Slater, A, Hill, SJ, Poulter, N, Thomas, S & Morgan, N 2019, 'Optimised insert design for improved single-molecule imaging and quantification through CRISPR-Cas9 mediated knock-in', Scientific Reports, vol. 9, no. 1, 14219. https://doi.org/10.1038/s41598-019-50733-9

Hansjosten, I, Rapp, J, Reiner, L, Vatter, R, Fritsch-Decker, S, Peravali, R, Palosaari, T, Joossens, E, Gerloff, K, Macko, P, Whelan, M, Gilliland, D, Ojea-Jimenez, I, Monopoli, MP, Rocks, L, Garry, D, Dawson, K, Röttgermann, PJF, Murschhauser, A, Rädler, JO, Tang, SVY, Gooden, P, Belinga-Desaunay, M-FA, Khan, AO, Briffa, S, Guggenheim, E, Papadiamantis, A, Lynch, I, Valsami-Jones, E, Diabaté, S & Weiss, C 2018, 'Microscopy-based high-throughput assays enable multi-parametric analysis to assess adverse effects of nanomaterials in various cell lines', Archives of toxicology, vol. 92, no. 2, pp. 633-649. https://doi.org/10.1007/s00204-017-2106-7

Morgan, N, Fletcher, S, Khan, A, Pisarev, A, Pisareva, V & Tcherepanov, A 2018, 'Role of the novel endoribonuclease SLFN14 and its disease causing mutations in ribosomal degradation', RNA, vol. 24, no. 7, pp. 939-949. https://doi.org/10.1261/rna.066415.118

Smith, C, Raslan, Z, Parfitt, L, Khan, AO, Patel, P, Senis, YA & Mazharian, A 2018, 'TREM-like transcript 1: a more sensitive marker of platelet activation than P-selectin in humans and mice', Blood Advances, vol. 2, no. 16, pp. 2072-2078. https://doi.org/10.1182/ bloodadvances.2018017756

Thomas, S, Morgan, N, Pike, J, Simms, V & Khan, A 2017, 'CRISPR-Cas9 mediated labelling allows for single molecule imaging and resolution', Scientific Reports, vol. 7, 8450. https://doi.org/10.1038/s41598-017-08493-x

Khan, K, Naylor, A, Khan, A, Noy, P, Mambretti, M, Lodhia, P, Athwell, J, Korzystka, A, Buckley, C, Willcox, B, Mohammed, F & Bicknell, R 2017, 'Multimerin-2 is a ligand for group14 family C-type lectins CLEC14A, CD93 and CD248 spanning the endothelial pericyte interface: MMRN2 binds three group 14 family C-type lectins', Oncogene. https://doi.org/10.1038/onc.2017.214

Chapter

Poulter, N, Khan, A, Pallini, C & Thomas, S 2018, Single molecule localisation and structured illumination microscopy of platelet proteins. in Platelets and megakaryocytes: volume 4, advanced protocols and perspectives. vol. 4, Methods in Molecular Biology, vol. 1812, Humana Press. https://doi.org/10.1007/978-1-4939-8585-2

Review article

Khan, A & Pike, J 2020, 'Super-resolution imaging and quantification of megakaryocytes and platelets', Platelets. https://doi.org/10.1080/09537104.2020.1732321

View all publications in research portal