From cleared whole organ imaging to super-resolution imaging of cellular organisation, the COMPARE microscopy facility offers access to state of the art commercial and custom-made microscopy systems.
We provide support through comprehensive consultations, sample preparation advice, microscopy training, image acquisition, image processing, image analysis, preparation of data for publication and grant applications - elevating research projects to the next level.
The facility has expertise in generating high quality quantitative data using techniques such as FRET, TIRFM, STORM, PALM, SPT, FCS, SIM, Photometry, image processing and custom image analysis.
3D image of microtubules coloured by Z depth. Dirk Herten, ICVS.
TIRF/Single particle tracking
QUAD TIRF, SIM H-TIRF & ONI Nanoimager
Single particle tracking (SPT) of up to four different fluorescent molecules in living cells.
Tracking of receptor diffusion at the cell membrane. Davide Calebiro, IMSR.
Cellular or subcelluar light-sheet microscopy
Visualise dynamic processes inside cells in unprecedented temporal resolution.
Long time lapse imaging of living samples, isotropic imaging of spheroids & organoids.
3D image of cytoskeletal proteins and nuclei. Deirdre Kavanagh, COMPARE Imaging.
Large volume light-sheet microscopy
Cleared mouse embryo showing developing organs and vasculature. Werther Vecchiato, ICVS.
Molecular interactions and dynamics
Perform intensity based FRET and calcium imaging in living cells with sub-millisecond temporal resolution.
Fluorescent Fluctuation Spectroscopy
Fluorescence spectroscopy of molecular dynamics, concentrations and interactions.
Auto and cross-correlation curves reveal protein diffusion and interactions rates. Deirdre Kavanagh, COMPARE Imaging.
- Virtual machines
- 8 Analysis Workstations
- Open source expertise
- High memory GPU compute node
- Arivis Vision 4D software
- Huygens Deconvolution software
Topological data analysis of single molecule data. Jeremy Pike,COMPARE Imaging.