Multiple Biofluid and Tissue Types, From Sample Preparation to Analysis Strategies for Metabolomics

Next course date: 6-8 December 2017

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This 3-day course will provide a comprehensive theoretical overview and hands-on training for the range of methods applied for sample preparation of complex biological samples for analysis applying LC-MS analysis. The course will be led by experts in the field to illustrate the different approaches that are available to analyse a range of biological samples and apply complementary liquid chromatography approaches to maximise the coverage of the metabolome. The course will be taught by experts in metabolomics who have experience of the analysis of microbial, plant and mammalian samples.

Topics covered:

  • Introduction to dealing with the complexity of biological samples using LC-MS.
  • Overview of different sample collection, sample quenching and sample extraction methods.
  • The challenges of working with cellular and tissue samples.
  • Overview of different LC methods including HILIC and reversed phase methods.
  • Hands-on sample preparation of blood, urine, cell and tissue samples. Monophasic and biphasic solvent extraction methods to target polar and non-polar metabolites.
  • SPE and liquid-liquid sample clean-up methods
  • Hands-on HILIC and reversed-phase liquid chromatography
  • Hands-on LC-MS analysis for untargeted studies (maximum of 4 people)
  • Overview of data analysis and metabolite identification
  • Problem solving and tips and tricks session with the experts

Cost:

  • Early bird Registration - £1040
  • Standard Registration - £1155
  • NERC/BBSRC/MRC Bursary - £0
    (for bursary information please visit Trainee Information)

Level: The course is aimed at PhD students and post-doctoral researchers who have been actively applying metabolomics for a minimum of 6 months. If individuals would like to take the course but do not have the recommended level of experience please contact the course administrator for advice.

Preview the full programme here

For further information about this course please contact David Epps.

Please read the Birmingham Metabolomics Training Centre’s Conditions and Cancellation Policy prior to registering.