Dr Lee’s research focuses on the regulation of gene expression in bacteria, with particular interest in understanding how the organized bacterial chromosome impacts on, and is impacted by, transcription events. In addition, he is interested in how horizontally acquired genes, particularly transcription factors, impact upon gene expression.
BSc (University of Birmingham)
PhD (University of Birmingham)
David graduated from the University of Birmingham with a degree in Biochemistry in 1996. Keen to pursue a career in research he joined Steve Busby’s research group in the School of Biosciences, studying the biochemical mechanisms by which bacteria adapt to growth without oxygen, and completed his PhD in 2000. Eager to stay and improve on his Birmingham accent, David has since developed several independent research streams and novel technologies in the field of bacterial genetics. In 2011, David was appointed as a Teaching and Research Fellow in the School of Biosciences.
Undergraduate Year 2:
Lectures and Practical - Bacterial Genetics BIO265
Practical Applications of Molecular Biotechnology BIOM12
Core Concepts and Skills in Microbiology BIOM21
Final year undergraduate, MRes and MSc research projects
PhD studentships are awarded each year competitively within the School of Biosciences. Funding options are also available for international students including a studentship from the Darwin Trust of Edinburgh.
David is always keen to discuss projects with potential candidates – please contact him directly.
Research theme within the School of Biosciences: Microbiology and Infection
The Bacterial Chromosome and Gene Regulation
The length of a bacterial chromosome far exceeds the length of the cell. To accommodate it, bacteria use a number of physical mechanisms to organize, fold and compact the chromosome. Typically, the chromosome occupies the majority of the intercellular space, and the highly compacted nature means that some regions are accessible, and some regions are buried. How this organization impacts upon gene regulation is a major focus in my laboratory and we are using a combination of molecular microbiological, proteomic and ChIP techniologies to ascertain how different genes, at different locations within the cell, are accessed by the transcription machinery.
The Impact of Horizontally Acquired DNA
Horizontal gene transfer accounts for the vast majority of emerging antibiotic resistance in bacteria. Of particular interest in my laboratory is the acquisition of large conjugative plasmids, which carry resistance to multiple antibiotics. We are investigating the impact of transcription factors carried on these plasmids on the host transcriptome.
David engages with the public and peers on scientific topics through social media outlets such as Twitter and Research Gate. He established and is a regular contributor to a weblog and a Twitter feed for the Institute of Microbiology and Infection (IMI), at the University of Birmingham.
David is co-organiser of the IMI summer school: a week long course providing year 12/13 A-level students with the opportunity to get hands-on practical experience of Microbiology at the University of Birmingham.
Bingle LE, Constantinidou C, Shaw RK, Islam MS, Patel M, Snyder LA, Lee DJ, Penn CW, Busby SJ, Pallen MJ. (2014) Microarray Analysis of the Ler Regulon in Enteropathogenic and Enterohaemorrhagic Escherichia coli Strains. PLoS One. PMID:24454682
Capeness MJ, Lambert C, Lovering AL, Till R, Uchida K, Chaudhuri R, Alderwick LJ, Lee DJ, Swarbreck D, Liddell S, Aizawa S, Sockett RE. (2013) Activity of Bdellovibrio Hit Locus Proteins, Bd0108 and Bd0109, Links Type IVa Pilus Extrusion/Retraction Status to Prey-Independent Growth Signalling. PLoS One. PMID: 24224002
Lee DJ, Busby SJ. (2012) Repression by cyclic AMP receptor protein at a distance. MBio. PMID 22967981
Lee DJ, Minchin SD, Busby SJ. (2012) Activating transcription in bacteria. Annu Rev Micro. PMID 22726217
Sánchez-Romero MA, Lee DJ, Sánchez-Morán E, Busby SJ. (2012) Location and dynamics of an active promoter in Escherichia coli K-12. Biochem J. PMID 21936772