Steve Thomas is a British Heart Foundation Research Fellow working with Professor Steve Watson in the Institute of Biomedical Research.
His main research interests centre around the actin cytoskeleton in platelets and megakaryocytes and fluorescence microscopy imaging.
The overall aim of my research is to investigate actin dynamics and the proteins that regulate these processes, in megakaryocytes and platelets. We use a variety of techniques to tackle these issues, with a focus on high resolution fluorescence microscopy. We are applying widefield, confocal, TIRF (total internal reflection fluorescence) and super-resolution microscopy to enable us to image actin dynamics of megakaryocyte or platelets as they activate and interact with their environment. Visualising the dynamics of proteins in live platelets will help us characterise the cytoskeleton in these cells in more detail. For example, we have recently identified novel structures in platelets called actin nodules and are using super-resolution microscopy to understand their structure and function.
Schachtner H, Calaminus SD, Thomas SG and Machesky LM (2013) Podosomes in adhesion, migration, mechanosensing and matrix remodelling. Cytoskeleton (Hoboken) 70(10):572-89
Schachtner H, Calaminus SD, Sinclair A, Monypenny J, Blundell MP, Leon C, Holyoake TL, Thrasher AJ, Michie AM, Vukovic M, Gachet C, Jones GE, Thomas SG, Watson SP and Machesky LM (2013) Megakaryocytes assemble podosomes that degrade matrix and protrude through basement membrane. Blood 121(13):2542-52
Davies A, Lewis DJ, Watson SP, Thomas SG and Pikramenou Z (2012) pH-controlled delivery of luminescent europium nanoparticles into platelets. Proc Natl Acad Sci USA 109(6):1862-7
Thomas SG, Calaminus SD, Machesky LM, Alberts AS and Watson SP (2011) G-protein coupled and ITAM receptor regulation of the formin FHOD1 through Rho kinase in platelets. J Thromb Haemost 9(8):1648-51
Mazharian A, Thomas SG, Dhanjal TS, Buckley CD and Watson SP (2010) Critical role of Src-Syk-PLCg2 signalling in megakaryocyte migration and thrombopoiesis. Blood 116(5):793-800
Senis YA, Tomlinson MG, Ellison S, Mazharian A, Lim J, Zhao Y, Kornerup KN, Auger JM, Thomas SG, Dhanjal T, Kalia N, Zhu JW, Weiss A and Watson SP (2009) The tyrosine phosphatase CD148 is an essential positive regulator of platelet activation and thrombosis. Blood 113(20):4942-54
Protty MB, Watkins NA, Colombo D, Thomas SG, Heath VL, Herbert J, Bicknell R, Senis YA, Ashman LK, Berditchevski F, Ouwehand WH, Watson SP and Tomlinson MG (2009) Identification of Tspan9 as a novel platelet tetraspanin. Biochem J 417(1):391-400
Calaminus SD, Thomas SG, McCarty OJ, Machesky LM and Watson SP (2008) Identification of a novel, actin-rich structure, the actin nodule, in the early stages of platelet spreading. J Thromb Haemost 6(11):1944-52