This Nikon system is a dual TIRF and laser scanning confocal (A1R) system, equipped with a Ti inverted microscope, Perfect Focus System (PFS), piezo-z, multi laser beds, a full in vivo imaging set up (T- and CO2- controlled within an incubation chamber) and an inverted motorized stage suitable for live imaging of cells (well plates or Matek dishes).
The A1R laser scanning confocal system feautures, spectral detectors, 7 laser lines (405- 457- 476- 488- 514- 546- 633nm), a resonant scanner for fast processes in live cells, live ratiometric imaging, tiled imaging optical sectioning for specimens that extend beyond the field of view (e.g. plants, invertebrates, neurons, microfluidic devices), widefield detector with DIC for simultaneous detection of confocal and transmitted light images; time-lapse imaging module and lambda imaging for spectral characterisation.
The TIRF system feautures 3 laser lines (488- 546- 647nm), an Andor EMCCD camera and 2 powerful TIRF lenses. The Nikon system is located on the 8th floor of the Biosciences building.