Seahorse XFe24 Analyzer

Seahorse XFe24 Analyzers measure oxygen consumption rate and extracellular acidification rates of live cells in a 24-well plate format. 

Rates of oxygen consumption and extracellular acidification are indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples. 

Features

  • Real-time results - this integrated system reports metabolic rates in intervals between 5-8 minutes, without sample extraction or labelling. Agilent Seahorse Wave software controls the instrument and performs rate measurements in real-time for same day results.
  • Live cell responses – detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing while maintaining a physiological temperature for your samples.
  • Sensitive assays – analyse as few as 10,000 cells per well in the custom 24-well plate.  Cell number requirements vary by cell type; consult the facility manager for more details. The 24-well microplate and system has the capacity for larger and/or more metabolically active samples than the 96-well system.
  • Compatible with a variety of samples due to a precision-controlled heating tray, which maintains temperatures between 16-42oC.
  • Analyse the function of 3D cell cultures, isolated pancreatic islets or other mobile samples with the XFe24 islet capture plate.
  • Create assay protocols and analyse data with Seahorse Wave software 

Specifications of XFe24 Analyzer

Specification  Detail
Number of assay wells 20
Microchamber volume  7μL
XFe24 Controller  Combination windows 7 computer and touchscreen display with full assay design, control and analysis capability
Software  Design and analyse assay templates on controller or Wave desktop (PC only).

Data can be exported into Microsoft Excel and GraphPad Prism.  

Assay types the XFe24 is best for 
  • Small model organisms
  • 3D cell cultures
  • Islets
  • Larger Samples 
Key Advantages  Validated for assay temperatures 16-42ºC
Microplate format  24-well 

All Agilent Seahorse XF Analyzers Feature:

  • Compatibility with both adherent and suspension cells as well as isolated mitochondria and non-mammalian samples.
  • Ability to perform up to 4 independent automatic injections per well with mixing
  • Automated calculation of oxygen consumption and extracellular acidification rates
  • Simultaneous measurement of oxygen consumption and extracellular acidification in the same well
  • Analytical sensitivity for small sample sizes
  • Label-free detection in real time
  • Windows-compatible desktop analysis software (Wave) for plotting, reporting, analyzing, and exporting XF data

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