Seahorse XFe24 Analyzers measure oxygen consumption rate and extracellular acidification rates of live cells in a 24-well plate format.
Rates of oxygen consumption and extracellular acidification are indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.
Features
- Real-time results - this integrated system reports metabolic rates in intervals between 5-8 minutes, without sample extraction or labelling. Agilent Seahorse Wave software controls the instrument and performs rate measurements in real-time for same day results.
- Live cell responses – detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing while maintaining a physiological temperature for your samples.
- Sensitive assays – analyse as few as 10,000 cells per well in the custom 24-well plate. Cell number requirements vary by cell type; consult the facility manager for more details. The 24-well microplate and system has the capacity for larger and/or more metabolically active samples than the 96-well system.
- Compatible with a variety of samples due to a precision-controlled heating tray, which maintains temperatures between 16-42oC.
- Analyse the function of 3D cell cultures, isolated pancreatic islets or other mobile samples with the XFe24 islet capture plate.
- Create assay protocols and analyse data with Seahorse Wave software
Specifications of XFe24 Analyzer
Specification |
Detail |
Number of assay wells |
20 |
Microchamber volume |
7μL |
XFe24 Controller |
Combination windows 7 computer and touchscreen display with full assay design, control and analysis capability |
Software |
Design and analyse assay templates on controller or Wave desktop (PC only).
Data can be exported into Microsoft Excel and GraphPad Prism.
|
Assay types the XFe24 is best for |
- Small model organisms
- 3D cell cultures
- Islets
- Larger Samples
|
Key Advantages |
Validated for assay temperatures 16-42ºC |
Microplate format |
24-well |
All Agilent Seahorse XF Analyzers Feature:
- Compatibility with both adherent and suspension cells as well as isolated mitochondria and non-mammalian samples.
- Ability to perform up to 4 independent automatic injections per well with mixing
- Automated calculation of oxygen consumption and extracellular acidification rates
- Simultaneous measurement of oxygen consumption and extracellular acidification in the same well
- Analytical sensitivity for small sample sizes
- Label-free detection in real time
- Windows-compatible desktop analysis software (Wave) for plotting, reporting, analyzing, and exporting XF data
Find out more
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