Seahorse XFe96 Analyzer

Seahorse XFe96 Analyzers measure oxygen consumption rate and extracellular acidification rates of live cells in a 96-well plate format. 

Rates of oxygen consumption and extracellular acidification are indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.  

Features

  • Real-time results - this integrated system reports metabolic rates in intervals between 5-8 minutes, without sample extraction or labelling. Agilent Seahorse Wave software controls the instrument and performs rate measurements in real-time for same day results.
  • Live cell responses – detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing while maintaining a physiological temperature for your samples.
  • Flexible assay design- the 96-well plate format accommodates many conditions in a single run and is ideal for dose-response studies and compound screening.
  • High sensitivity- analyse as few as 5000 cells per well in the custom 96-well plate.  Cell number requirements vary by cell type; consult the facility manager for more details.
  • Compatible with a variety of samples due to the precision-controlled heating tray, which can maintain 16-42oC.
  • Analyse 3D samples such as spheroids and islets with the Seahorse XFe96 Spheroid Plate
  • Easily create assay protocols and analyze data with Seahorse Wave software

Specifications of XFe96 Analyzer

Specification  Detail
Number of assay wells 92
Microchamber volume  2μL
XFe24 Controller  Combination windows 7 computer and touchscreen display with full assay design, control and analysis capability
  • Software 
  • Design and analyse assay templates on controller or Wave desktop (PC only).
  • Data can be exported into Microsoft Excel and GraphPad Prism.  
Assay types the XFe24 is best for 
  • Phenotypic screening 
  • Testing multiple conditions in one plate
  • Does response studies
  • Spheroids 
  • Small sample volume
Key Advantages  Validated for assay temperatures 16-42ºC
Microplate format  96-well 

All Agilent Seahorse XF Analyzers Feature:

  • Compatibility with both adherent and suspension cells as well as isolated mitochondria and non-mammalian samples.
  • Ability to perform up to 4 independent automatic injections per well with mixing
  • Automated calculation of oxygen consumption and extracellular acidification rates
  • Simultaneous measurement of oxygen consumption and extracellular acidification in the same well
  • Analytical sensitivity for small sample sizes
  • Label-free detection in real time
  • Windows-compatible desktop analysis software (Wave) for plotting, reporting, analysing, and exporting XF data

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