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Metabolite Identification with the Q Exactive and LTQ Orbitrap Elite
Please email for registration information: bmtc@contacts.bham.ac.uk
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Course Overview
This 2-day course provides a hands-on approach to teach attendees about the latest techniques and tools available to perform metabolite identification in non-targeted metabolomics studies. The course is led by experts working within the field of metabolomics, and will include a significant proportion of hands-on experience of using mass spectrometers, software tools and databases. A maximum of four people will be working on each mass spectrometer (Q Exactive and LTQ-Orbitrap Elite) in a session.
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Topics Covered
- Importance of mass spectral interpretation
- Types of data which can be collected on the QE and LTQ-Orbitrap Elite (m/z, retention time, MS/MS, MSn)
- Conversion of raw data to molecular formula and putative metabolite annotations
- MS/MS experiments in metabolic phenotyping for on-line data acquisition using the QE (Data Dependent Analysis, Data Independent Analysis)
- MS/MS and MSn experiments for sample fractions using the LTQ-Orbitrap Elite
- Mass spectral libraries (using mzCloud)
- Searching mass spectral libraries
- Tools for mass spectral interpretation
- In silico fragmentation (using MassFrontier)
- Reporting standards for metabolite identification
- Question and answer session with the experts
Preview the full course programme here
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Level
This course is suitable for PhD students and post-doctoral researchers who have been actively applying metabolomics for a minimum of 6 months. If students or researchers would like to take the course but do not have the recommended level of experience please contact the course administrator for advice.
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Course Feedback
Based on feedback provided for this course
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100%
of attendees said this course is excellent or good
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100%
of attendees would recommend this course to a colleague
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"I enjoyed the practical part very much. "Learning by doing" is first of all very effective but the whole session was also a great opportunity to ask about some more specific practical parts of the metabolomics approach."
Rikke
"I enjoyed how hands on everything was - and how it was geared specifically towards the questions we had on our own research... Overall I thought it was excellent and I learned a ton."
Rachel
"The practical part was great and very dynamic. It was the first experience I had ever had and it resulted in a very promising contact with the new field."
Diego
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Early-bird Registration
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£755
Email for information: bmtc@contacts.bham.ac.uk
1 x Delegate
Rate expires: TBC
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£1,359
Email for information: bmtc@contacts.bham.ac.uk
2 x Delegates (10% discount)
Rate expires: TBC
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Standard Registration
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£840
Email for information: bmtc@contacts.bham.ac.uk
1 x Delegate
Rate expires: TBC
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£1,512
Email for information: bmtc@contacts.bham.ac.uk
2 x Delegates (10% discount)
Rate expires: TBC
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MRC Bursary Registration
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FREE
Please email for registration information: bmtc@contacts.bham.ac.uk
Rate expires: TBC
See our Trainee Information Page here for more information, terms and conditions.
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Please read the Birmingham Metabolomics Training Centre's Conditions and Cancellation Policy prior to registering.
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