Multiple Biofluid and Tissue Types, From Sample Preparation to Analysis Strategies for Metabolomics

School of Biosciences - University of Birmingham
Event cost
Monday 10 February (09:00) - Wednesday 12 February 2020 (15:30)


Multiple Biofluid and Tissue Types, From Sample Preparation to Analysis Strategies for Metabolomics

Next course run: 23 - 25 September 2020 | Register now (link to new course page)

Course Overview

This 3-day course provides a theoretical overview and hands-on training to apply multiple sample preparation and UPLC-MS methods to characterise the metabolomes of complex biological samples using the mass spectrometer (Xevo QToF G2-XS - a maximum of 4 people working on the instrument in a session). The course is led by experts in the field who have experience of the analysis of microbial, plant and mammalian samples, and illustrates the different approaches that are available to analyse a range of biological samples and applying complementary liquid chromatography approaches to maximise the coverage of the metabolome.

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Topics Covered

  • Introduction to dealing with the complexity of biological samples using UPLC-MS
  • Overview of different sample collection, sample quenching and sample extraction methods
  • The challenges of working with cellular and tissue samples
  • Overview of different UPLC methods including HILIC and reversed phase methods
  • Hands-on sample preparation of plasma, urine, cell and tissue samples
  • Monophasic and biphasic solvent extraction methods to target polar and non-polar metabolites
  • SPE and liquid-liquid sample clean-up methods
  • Hands-on HILIC and reversed-phase liquid chromatography
  • Hands-on UPLC-MS analysis for untargeted studies (maximum of 4 people)
  • Overview of data analysis and metabolite identification
  • Problem solving and tips and tricks session with the experts

Preview the full course programme here



The course is aimed at PhD students and post-doctoral researchers who have been actively applying metabolomics.

Testimonials 2

Course Feedback

Based on feedback provided for this course 2017-2019


of attendees said this course is excellent or good


of attendees would recommend this course to a colleague

"The course is wonderfully designed for the people who are already working in metabolomics and even for the new comers. Besides the course, I must say that the people involved (Prof Dunn, Cath, Ralf and David) were very friendly and helpful. They encouraged us to get in touch with them if we come across any difficulties which was very satisfying. I would highly recommend this course irrespective of your field (academic or industry)."

"Everyone was very nice and willing to help me out with the metabolomics issues I am currently having in my project/lab. Everything was very well explained and overall the course was very informative in terms of metabolomics principles and practicality."

"The course was amazing very informative, the staff were super helpful and extremely professional and nice."

"Attending this course was the best thing that could happen to me for my start into the field of metabolomics and I really enjoyed it."


Standard Registration


Registration closed

1 x Delegate

Rate expires: 9 February 2020


Registration closed

2 x Delegates (10% discount)

Rate expires: 9 February 2020


Please read the Birmingham Metabolomics Training Centre's Conditions and Cancellation Policy prior to registering.