Metabolite Identification with the Q Exactive and LTQ Orbitrap Elite

Location
School of Biosciences - University of Birmingham
Event cost
840
Dates
Thursday 2 April (09:00) - Friday 3 April 2020 (17:30)
Contact

bmtc@contacts.bham.ac.uk

Met ID

Metabolite Identification with the Q Exactive and LTQ Orbitrap Elite

2 - 3 April 2020 | Email us to register for this course : bmtc@contacts.bham.ac.uk
 

Course Overview

This 2-day course provides a hands-on approach to teach attendees about the latest techniques and tools available to perform metabolite identification in non-targeted metabolomics studies. The course is led by experts working within the field of metabolomics, and will include a significant proportion of hands-on experience of using mass spectrometers, software tools and databases. A maximum of four people will be working on each mass spectrometer (Q Exactive and LTQ-Orbitrap Elite) in a session.

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Topics Covered

  • Importance of mass spectral interpretation
  • Types of data which can be collected on the QE and LTQ-Orbitrap Elite (m/z, retention time, MS/MS, MSn)
  • Conversion of raw data to molecular formula and putative metabolite annotations
  • MS/MS experiments in metabolic phenotyping for on-line data acquisition using the QE (Data Dependent Analysis, Data Independent Analysis)
  • MS/MS and MSn experiments for sample fractions using the LTQ-Orbitrap Elite
  • Mass spectral libraries (using mzCloud)
  • Searching mass spectral libraries
  • Tools for mass spectral interpretation
  • In silico fragmentation (using MassFrontier)
  • Reporting standards for metabolite identification
  • Question and answer session with the experts

Preview the full course programme here

Level

Level 

This course is suitable for PhD students and post-doctoral researchers who have been actively applying metabolomics for a minimum of 6 months. If students or researchers would like to take the course but do not have the recommended level of experience please contact the course administrator for advice.

Testimonials 2

Course Feedback

Based on feedback provided for this course 2017-2019

100%

of attendees said this course is excellent or good

100%

of attendees would recommend this course to a colleague


"I enjoyed the practical part very much. "Learning by doing" is first of all very effective but the whole session was also a great opportunity to ask about some more specific practical parts of the metabolomics approach."
Rikke

"I enjoyed how hands on everything was - and how it was geared specifically towards the questions we had on our own research... Overall I thought it was excellent and I learned a ton."
Rachel

"The practical part was great and very dynamic. It was the first experience I had ever had and it resulted in a very promising contact with the new field."
Diego

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Standard Registration

£840

Email us to register for this course : bmtc@contacts.bham.ac.uk

1 x Delegate

 

£1,512

Email us to register for this course : bmtc@contacts.bham.ac.uk

2 x Delegates (10% discount)

 

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MRC Bursary Registration

FREE

Email us to register at this rate

Rate expires:  31 March 2020

See our Trainee Information Page here for more information, terms and conditions.

 


Please read the Birmingham Metabolomics Training Centre's Conditions and Cancellation Policy prior to registering.

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