Metabolite identification with the Q Exactive and LTQ Orbitrap Elite

Register Now for 22-23 November 2018

Overview

This 2-day course provides a hands-on approach to teach attendees about the latest techniques and tools available to perform metabolite identification in non-targeted metabolomics studies. The course is led by experts working within the fields of metabolomics, and will include a significant proportion of hands-on experience of using mass spectrometers, software tools and databases. A maximum of four people will be working on each mass spectrometer (Q Exactive and LTQ-Orbitrap Elite) in a session.

Topics covered

  • Importance of mass spectral interpretation
  • Types of data which can be collected on the QE and LTQ-Orbitrap Elite (m/z, retention time, MS/MS, MSn)
  • Conversion of raw data to molecular formula and putative metabolite annotations
  • MS/MS experiments in metabolic phenotyping for on-line data acquisition using the QE (Data Dependent Analysis, Data Independent Analysis)
  • MS/MS and MSn experiments for sample fractions using the LTQ-Orbitrap Elite
  • Mass spectral libraries (using mzCloud)
  • Searching mass spectral libraries
  • Tools for mass spectral interpretation
  • In silico fragmentation (using MassFrontier)
  • Reporting standards for metabolite identification
  • Question and answer session with the experts

Level

The course is suitable for PhD students and post-doctoral researchers who have been actively applying metabolomics for a minimum of 6 months. If students or researchers would like to take the course but do not have the recommended level of experience please contact the course administrator for advice.

Preview the full programme here


Testimonials

"I enjoyed how hands on everything was - and how it was geared specifically towards the questions we had on our own research... Overall I thought it was excellent and I learned a ton."

 - Rachel

Attended Metabolite identification with the Q Exactive and LTQ Orbitrap Elite April 2018

 

"The practical part was great and very dynamic. It was the first experience I had ever had and it resulted in a very promising contact with the new field."

 - Diego

Attended Metabolite identification with the Q Exactive and LTQ Orbitrap Elite July 2017


Cost

 Early-bird Registration £755
 Standard Registration £840
 NERC Bursary Registration

£0 (for bursary information please visit Trainee Information)

Register Now for 22-23 November 2018

For further information about this course please contact David Epps.

Please read the Birmingham Metabolomics Training Centre’s Conditions and Cancellation Policy prior to registering.