Dr Jo Parish BSc, PhD

Dr Jo Parish

Institute of Cancer and Genomic Sciences
Senior Lecturer

Contact details

Address
Room WX1.22, IBR West
Institute of Cancer and Genomic Sciences
University of Birmingham
Edgbaston
Birmingham
B15 2TT

Dr Jo Parish was awarded a Royal Society University Research Fellowship in 2007 and was appointed Senior Lecturer in Institute of Cancer and Genomic Sciences at the University of Birmingham in 2012.

The main focus of Jo’s research is the study of novel virus-host interactions that are important for viral pathogenesis and persistence. Jo has a longstanding interest in the molecular biology of human papillomavirus (HPV) life cycle and uses state-of-the art model systems and technologies to study HPV replication, persistence and transcriptional control.  

Qualifications

  • PhD in Biochemistry, University of Bristol, 2002
  • BSc in Biochemistry, University of Bristol, 1998

Biography

Dr Jo Parish completed her Ph.D. at University of Bristol in 2002, during which she elucidated the mechanism of the induction of apoptosis by the HPV E2 protein under the supervision of Dr. Kevin Gaston. Following her Ph.D. studies, she moved to the University of Massachusetts Medical School, USA to work with Professor Elliot Androphy. During this time, Joanna continued her study of the papillomavirus E2 protein and discovered several novel virus-host interactions including an interaction with the cellular DNA helicase ChlR1. Her work elegantly demonstrated that ChlR1 is required for the maintenance and persistence of papillomavirus by tethering viral genomes to cellular chromatin during mitosis and was published in Molecular Cell. In 2007, Joanna was awarded a highly competitive Royal Society University Research Fellowship and returned to the UK to establish her independent research group at the University of St Andrews. Joanna was recruited to the University of Birmingham, UK in 2012 and is Group Leader of the HPV Persistence Group. Joanna is a passionate mentor of early career researchers and as an elected member of the Microbiology Society Virus Division continues to promote virology on a national and international stage. 

Dr Parish’s research is currently focused on the molecular biology of transcriptional control and persistence of HPV, hepatitis B virus (HBV) and other small DNA viruses. Her recent research has identified an essential role for the host transcription factors CTCF and YY1 in the control of differentiation-dependent virus gene expression and her research has unravelling the mechanism of HPV transcription control through epigenetic regulation. She continues to address long-standing questions about HPV gene expression control and replication using novel and state-of-the-art technologies. 

Teaching

  • Deputy module organiser BMedSci: Viruses Threats and Defences
  • Lecturer and Tutor for BMedSci years 1 and 2 ‘Infection and Immunity’
  • Lecturer for BMedSci (Clinical Sciences)
  • MBChB Tutor: ‘Cancer: Causes to Cures’
  • MBChB Personal Academic Tutor

Postgraduate supervision

Dr Jo Parish has supervised more than 20 postgraduate students in areas of virology and cancer biology. Many of these students now hold posts at prestigious national and international research intitutes.

Jo is interested in supervising doctoral research students in the following areas:

  • Regulation of HPV gene expression control
  • Regulation of HPV replication and persistence
  • Regulation of HBV gene expression control

If you are interested in studying any of these subject areas please contact Jo using the details above or for any general doctoral research enquiries, please email: dr@contacts.bham.ac.uk or call +44 (0)121 414 5005

Research

Research Theme

Dr Parish’s research programme aims to understand the mechanistic underpinnings of virus gene expression control, replication and persistence using physiological models of human papillomavirus (HPV) and hepatitis B virus (HBV) infections.

Current projects:

Virus-host interactions important for the control of HPV gene expression and HPV-induced carcinogenesis
Using primary keratinocyte-based models of HPV infections, we are characterizing novel host factors that are important for virus oncogene expression and differentiation-dependent regulation of HPV gene expression. Our collaborative work with Dr Sally Roberts has highlighted an important interaction between HPV and the host cell transcriptional regulator CTCF in the control of virus oncogene expression and we are currently analysing this interplay in HPV-induced disease progression. Work is supported by Cancer Research UK and the Medical Research Council.

Viral manipulation of host cell gene expression
Using state-of-the-art technologies such as RNA-Seq, ChIP-Seq and FAIRE-Seq we are discovering how HPV manipulates transcriptional control of host cell genes to support virus life cycle. These studies are increasing our understanding of how HPV infection contributes to cancer development and will inform the design of novel diagnostic and prognostic biomarkers. Work is supported by Cancer Research UK and the Medical Research Council.

Virus-host interactions important for the control of HBV gene expression.
We are studying host cell-mediated regulation of HBV gene expression using physiologically-relevant models of HBV infection and state-of-the-art technologies. Work is supported by the Medical Research Council.

Member of the Birmingham Centre for Genome Biology   

Member of the Cancer Research UK Birmingham Centre  

Other activities

  • Editor of Journal of General Virology
  • Editorial Board Member of Viruses
  • Elected member of the Microbiology Society Virus Division Committee (2015-2018)
  • Lecturer in Medical Science, University of St Andrews (2007-2012)
  • Member of the American Society for Microbiology
  • Member of the British Society for Cell Biology
  • Member of the Biochemical Society 
  • Member of the Heinrich-Pette Institute for Medical Virology, Hamburg Scientific Advisory Board

Public Engagement

Jo has been a STEM ambassador since 2011 and frequently participates in public engagement activities including Café Scientifique presentations, participation in the Royal Society Summer Science Exhibition, Schools visits/workshops/careers fairs and Judge of the National Science Competition at The Big Bang.

In 2016, Jo presented her research area at the University of Birmingham Life Sciences in Six event. 

Publications

Most recent publications (full list of publications available at Google Scholar)

* Pentland I., *Campos-Leon K., Cotic M., Davies K-J., Wood C.D., Groves I., Burley M., Coleman N., Stockton J., Noyyert B., Beggs A., West M.J., Roberts S., Parish J.L.  Disruption of CTCF-YY1 dependent looping of the human papillomavirus genome activates differentiation-induced viral oncogene transcription. (2018) PLoS Biology, 16(10): e2005752. * Equal contribution.

Llewellyn M., Gordon N.S., Abbotts B., James N.D., Zeegers M.P., Cheng K.K., Macdonald A., Roberts S., Parish J.L., Ward D.G., Bryan R.T. Defining the frequency of human papillomvarius and polyomavirus infection in urothelial bladder tumours. (2018) Scientific Reports. 8, e11290

Morgan E.L., Wasson C.W., Hanson L., Kealy D., Pentand I., McGuire V., Scarpini C., Coleman N., Arthur S.C., ParishJ.L., Roberts S., Macdonald A. STAT3 activation by E6 is essential for the differentiation-dependent HPV18 life cycle in primary keratinocytes (2018) PLoS Pathogens. 14(4), e1006975

Anayannis N., Schlecht N.F., Ben-Dayan M., Smith R.V., Belbin T.J.,Ow T.J., Blakaj D.M., Burk R.D., Leonard S.M., Woodman C.B., Parish J.L., Prystowsky M.B. Detection of HPV16 E2 Disruption in HPV16 positive Head and Neck Squamous Cell Carcinoma (2018) PLoS One. 13 (2) e10191581

Marsh E., Delury C., Davies N., Weston C., Miah M.L., Banks L. Parish J.L., Higgs M., Roberts S. Mitotic control of human papillomavirus genome-containing cells is regulated by the function of the PDZ-binding motif of the E6 oncoprotein. Oncotarget. 2017; DOI: 10.18632/oncotarget.14469

Campos-Leon K., Wijendra K., Siddiqa A., Pentland I., Feeney K., Knapman A., Davies R., Androphy E., Parish J.L. Association of human papillomavirus type 16 E2 with Rad50-interacting protein 1 enhances viral DNA replication: Rint1 participates in HPV DNA replication. Journal of Virology. 2017; 91(5):1-15.

Harris L., McFarlane L., Campos-Leon K., Roberts S., Parish J.L. The cellular DNA helicase ChIR1 regulates chromatin and nuclear matrix attachment of the human papillomavirus type 16 E2 protein and high copy viral genome establishment: ChIR1 regulates the chromatin association of HPV16 E2. Journal of Virology. 2017; 91(1):1-16.

Siddiqa A., Campos-Leon K., James C., Roberts S., Parish J.L. The human papillomavirus type 16 L1 protein interacts with E2 and enhances E2-dependent replication and transcription activation. Journal of General Virology. 2015; 96, 2274-2285. DOI: 10.1099/vir.0.000162

Pentland I., Parish, J.L. Targeting CTCF to control virus gene expression: A common theme amongst diverse DNA viruses. (2015) Viruses. 7(7):3574-85. doi: 10.3390/v7072791

Paris C., Pentland I., Groves I., Roberts D.C., Coleman N., Roberts S., Parish J.L. CCCTC-Binding Factor Recruitment to the Early Region of the Human Papillomavirus Type 18 Genome Regulates Viral Oncogene Expression. Journal of Virology. 2015;89(9):4770-85. Cover Image DOI: 10.1128/JVI.00097-15

Siddiqa A., Zainab M., Qadri I., Bhatti M.F., Parish J.L. Prevalence and genotyping of high risk human papillomavirus in cervical cancer samples from Punjab, Pakistan. Viruses 2014; 6, 2762-2777. DOI: 10.3390/v6072762

Prystowsky M. B., Feeney K. M., Kawachi N., Montagne C., Willmott M. K. S., Wasson C. W., Antkowiak M., Loudig O., Parish J. L. Inhibition of Plk1 and Cyclin B1 expression results in panobinostat-induced G2 delay and mitotic defects. Scientific Reports. 2013;3:2640. DOI: 10.1038/srep02640

Feeney K. M., Saade A., Okrasa K., Parish J. L. In vivo analysis of the cell cycle dependent association of the bovine papillomavirus E2 protein and ChlR1. Virology. 2011;414:1-9. DOI: 10.1016/j.virol.2011.03.015

Prystowsky M. B., Adomako A., Smith R. V., Kawachi N., McKimpson W., Atadja P., Chen Q., Schlecht N., Parish J. L., Childs G., Belbin T. The histone deacetylase inhibitor LBH589 inhibits expression of mitotic genes causing G2/M arrest and cell death in head and neck squamous cell carcinoma cell lines. Journal of Pathology. 2009;218 (4):467-477.

Parish J. L., Bean A.M., Park R.B., Androphy E.J. ChlR1 is required for loading papillomavirus E2 onto mitotic chromosomes and viral genome maintenance. Molecular Cell. 2006;24(6), 70-76.

Parish J. L., Rosa J., Wang X., Lahti J., Doxsey S. J., Androphy E. J. The DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells. Journal of Cell Science. 2006;119, 4857-4865.

Parish J. L., Kowalczyk A., Chen H-T., Roeder G., Sessions R., Buckle M., Gaston K.  The E2 proteins from high- and low-risk HPV type differ in their ability to bind p53 and induce apoptotic cell death. Journal of Virology. 2006;80 (9), 4580-90.