BetaCell Birmingham provides live shipments of stem cell-generated beta cells (SBCs) that adhere to our flow cytometry-based quality controls.
In insulin promoter driven GFP (INS-GFP) lines, the cells commence GFP expression by day12 (pancreatic endoderm), which becomes prominent by day15-day17 (early endocrine, immature SBC). The cells reach their full maturity by day30. Our standard days for shipment are either day 23, coinciding with 35-60% GFP positive cells, or day 30, marking the completion of our protocol with mature insulin-secreting cells.
We ensure a minimum of 35% GFP(+)ve cells, with our Hes3 cells consistently yielding between 40-50% GFP(+) cells.
These quality-controlled cells are ready to contribute to your research, offering reliability and consistency. In addition to SBCs, we offer a range of complementary research materials. You can inquire about obtaining RNA, DNA, and fixed tissue samples to further enhance your studies. Feel free to reach out to us for more details or specific requests.
Flow-cytometry based quality controls for pancreatic progenitor cells and mature stem cell generated beta cells clusters (A) Brightfield (left two) and fluorescent (GFP) images of Hes3 INS-GFP embryonic stem cells undergoing beta cell differentiation protocol in Akerman Lab. Stem cell clusters self-aggregate when they reach critical concentration in rotation cultures: the cells become GFP positive as insulin starts to be expressed (day 10 onwards). (B) Measurements of mature/processed insulin levels in SBCs at d23. Representative flow cytometry dot-plots showing expression of C-peptide in stem cells (day0) or maturing beta cells (day23). C-peptide results from the processing of the preproinsulin peptide into mature insulin (equimolar ratios to insulin) (C) Measurements of insulin transcription levels in SBCs at d23. Representative flow cytometry dot-plots showing insulin promoter driven GFP expression in stem cells (day0) or maturing endocrine cells (day23).