Analysers
BD LSR FORTESSA X-20
The BD LSRFortessa™ X-20 cell analyser delivers high-performance multicolour analysis. The analyser has 5 lasers to detect up to 16 parameters simultaneously to support ever increasing demands in multicolour flow cytometry. The acquisition software is DIVA.
| Fortessa 2 |
|---|
|
Laser
|
Filter
|
Suggested Dye(s)
|
|
405 (Violet)
|
450/50
|
Pacific Blue,
BV450, CFP, Dapi
|
|
|
525/50
|
Pacific Orange,
BV510, AmCyan
|
|
|
610/20
|
BV605
|
|
|
670/30
|
BV650
|
|
|
710/50
|
BV711
|
|
|
780/60
|
BV786
|
|
488 (Blue)
|
530/30
|
FITC, GFP
|
|
|
670/30
|
PerCP, PerCPCy5.5
|
|
561 (Yellow)
|
586/15
|
PE, DsRed
|
|
|
610/20
|
PE-TxRed, mCherry, RFP, PI
|
|
|
670/30
|
PE-Cy5
|
|
|
710/50
|
PE-Cy5.5
|
|
|
795/71
|
PE-Cy7
|
|
642 (Red)
|
671/30
|
APC, AF647
|
|
|
722/44
|
AF700, Dylight680
|
|
|
795/70
|
APC-Cy7
|
| Fortessa 1, 2 & 4 |
|---|
|
Laser
|
Filter
|
Suggested Dye(s)
|
|
355 (UV)
|
740/35
|
BUV737
|
|
|
379/28
|
BUV395
|
|
405 (Violet)
|
450/50
|
Pacific Blue, BV450, BV421, CFP, Dapi
|
|
|
525/50
|
Pacific Orange, BV510, AmCyan
|
|
|
610/20
|
BV605
|
|
|
670/30
|
BV650
|
|
|
710/50
|
BV711
|
|
|
780/60
|
BV786
|
|
488 (Blue)
|
530/30
|
FITC, GFP
|
|
|
670/30
|
PerCP, PerCPCy5.5
|
|
561 (Yellow)
|
586/15
|
PE, DsRed
|
|
|
610/20
|
PE-TxRed, mCherry, RFP, PI
|
|
|
670/30
|
PE-Cy5
|
|
|
710/50
|
PE-Cy5.5
|
|
|
795/71
|
PE-Cy7
|
|
642 (Red)
|
671/30
|
APC, AF647
|
|
|
722/44
|
AF700, Dylight680
|
|
|
795/70
|
APC-Cy7
|
Beckman Coulter CytoFLEX S
The high-performance CytoFLEX system utilizes three excitation lines with independent, alignment-free focusing optics, simultaneous 13-parameters and two scatter parameters. It has superior sensitivity and resolution for excellent fluorescence and nanoparticle detection.
The instrument also possesses a plate loader module, enabling the acquisition of samples within standard flat-bottom, U-bottom and V-bottom 96-well plates. The acquisition software is CytExpert.
| Beckman CoulterCytoFLEX S |
|---|
|
Laser
|
Filter
|
Suggested Dye(s)
|
|
405 (Violet)
|
450/50
|
Pacific Blue, BV450, eFluor450, BV421
|
|
|
525/40
|
Pacific Orange, BV510, AmCyan
|
|
|
610/20
|
BV605, Qdot605, Violet610
|
|
|
660/10
|
BV650, Qdot695, Violet660
|
|
610 (Yellow)
|
610/20
|
ECD, mCherry
|
|
|
585/42
|
PE, DsRed
|
|
|
390/50
|
PECy5.5, PI
|
|
|
780/60
|
PeCy7
|
|
638 (Red)
|
660/20
|
APC, AF647, eFlour660
|
|
|
712/25
|
AF700, Alexa Fluor 700, Dylight680
|
|
|
780/60
|
APC eFluor780, APC-Cy7, APC-A750
|
|
488 (Blue)
|
525/40
|
FITC, GFP, Alexa 488, CFSE
|
|
|
585/42
|
PercP
|
ThermoFisher Attune NxT
The Attune NxT Flow Cytometer system makes flow cytometry available to both new and experienced researchers. It has 2 lasers and can detect 7 parameters and two scatter parameters. I also has available an auto sampler to acquire from 96 well plates. The acquisition software is Invitrogen Attune NxT.
| ThermoFisher Attune NxT |
|---|
|
Laser
|
Filter
|
Suggested Dye(s)
|
|
585 (Yellow)
|
YL1 585/16
|
PE, Alexa Fluor 568, PI, DsRed
|
|
|
YL2 620/15
|
PE-Texas Red, mCherry
|
|
|
YL3 695/40
|
PECy5.5, Alexa Fluor 700, PercP Cy5.5
|
|
|
YL4 780/60
|
PeCy7, Qdot800
|
|
488 (Blue)
|
BL1 530/30
|
FITC, GFP, Alexa 488, CFSE
|
|
|
BL2 590/40
|
PI, PE-Texas Red, PE-Alexa Fluor 610
|
|
|
BL3 695/40
|
PercP, PECy5.5, PercP Cy5.5
|
Imaging Flow Cytometer
Amnis ImageStream X Mk II
The Amnis ImageStream Mk II is a 6-channel imaging flow cytometer which combines the speed and sensitivity of flow cytometry with the detailed imagery and functional insights of microscopy. By collecting large numbers of digital images per sample and providing a numerical representation of image-based features, the instrument combines the per cell information content provided by standard microscopy with the statistical significance afforded by large sample sizes common to standard flow cytometry. The acquisition software is INSPIRE whereas the analysis software is IDEAS.
|
Amnis ImageStream Mk II Standard Detector Configuration
|
|
Channel
|
Filter (nm)
|
Excitation Laser (nm)
|
|
405
|
488
|
642
|
785
|
|
1
|
435 – 505
|
*DAPI, BV421, AF405,
Hoechst, PacBlue, CascadeBlue, eFluor450, DyLight405, CFP, Live/Dead Violet
|
|
|
|
|
2
|
505 – 560
|
*BV510, PacOrange, CascadeYellow, AF430, eFluor525, QD525
|
FITC, AF488, GFP, YFP, DyLight488, PKH67, Syto13, SpectrumGreen, LysoTrackerGreen, MitoTrackerGreen
|
|
|
|
3
|
560 -595
|
*QD565, QD585, eFluor565
|
PE, PKH26, DSRed, mOrange, CellMask/CellTracker/SYTOX Orange, Cy3
|
|
|
|
4
|
595 – 642
|
*QD625, eFluor625, BV605
|
PE-TexRed, ECD, PE-AF610, RFP, QD625, eFluor625
|
|
|
|
5
|
642 – 745
|
*QD705, eFluor700, BV711
|
PE-Cy5, PE-AF647, 7AAD, PI, PerCP, PerCP-Cy5.5, DRAQ5, QD705, eFLuor650, FuraRedlo, DRAQ5, LDS 751
|
APC, AF647, AF660, AF680, DRAQ5, Cy5, DyLight649, DyLight680, PE-AF647, PE-Cy5, PerCP, PerCP-Cy5.5
|
|
|
6
|
745-785
|
*QD800, BV786
|
PE-Cy7, PE-AF750, QD800
|
APC-Cy7, APC-AF750, APC-H7, APC-eFluor750, Cy7, AF750, DyLight750, PE-Cy7, PE-AF750
|
SSC
|
Recommended dyes (based on optimal excitation and detection channels) are in boldface.
*Many dyes will excite by more than one laser, and this can increase cross camera compensation.
Ideal dyes are DAPI (or BV421), AF488, PE, PE-TxRed, AF647, SSC/BF
Cell Sorter
BD FACSAria™ Fusion
The BD FACSAria™ Fusion flow cytometer delivers exceptional multicolour performance. The fully integrated biosafety cabinet meets emerging operator and sample protection requirements as well as global standards for bio-protection. It has 5 lasers to detect up to 18 parameters simultaneously. It is capable of sorting samples in tubes or plates. With the capacity of 4 way sorting. The acquisition software is DIVA.
| FACSAria |
|---|
|
Laser
|
Filter
|
Suggested Dye(s)
|
|
355 (UV)
|
450/50
|
DAPI, Hoechst Blue
|
ARIA 1
|
|
|
670/25
|
BUV661
|
ARIA 1
|
|
|
379/28
|
BUV395
|
ARIA 2
|
|
|
740/35
|
BUV737
|
ARIA 2
|
|
405 (Violet)
|
450/50
|
Pacific Blue, BV450, BV421, CFP, Dapi
|
|
|
525/50
|
Pacific Orange, BV510, AmCyan
|
|
|
610/20
|
BV605
|
|
|
660/20
|
BV650
|
|
|
710/50
|
BV711
|
|
|
780/60
|
BV786
|
|
488 (Blue)
|
695/40
|
PerCPCy5.5
|
|
|
530/30
|
FITC
|
|
561 (Yellow/Green)
|
582/15
|
PE, DsRed
|
|
|
610/20
|
PE-TxRed, mCherry, RFP, PI
|
|
|
670/14
|
PE-Cy5
|
|
|
710/50
|
PE-Cy5.5
|
|
|
780/60
|
PE-Cy7
|
|
640 (Red)
|
780/60
|
APC-Cy7
|
|
|
733/45
|
AF700, Dylight680
|
|
|
670/30
|
APC
|
Mass Cytometer
Helios™ Mass Cytometer - CyTOF®
Helios™, a CyTOF® system, comprises the most recent advances in mass cytometry and is designed to provide a new and improved tool for bioanalytical single-cell detection and analysis. This high-performance mass cytometer, from the CyTOF family of instruments, enables the analysis of more than 40 markers and uniquely allows their quantitative determination with negligible spectral overlap, a result of exquisite resolution between mass detection channels.
Spectral Cytometer
Sony ID7000TM Spectral Cell Analyser
The ID7000™ spectral cell analyser supports high-parameter flow cytometry by delivering comprehensive information about heterogeneous cell populations, with high sensitivity to detect dim and rare populations.
The ID7000 expands the capabilities of cell analysis, as it is configured with 5 lasers. The system enables researchers to perform experiments using 44 colours or more, limited only by the fluorochromes available. Laser choices range from ultraviolet (355 nm) to red (637nm). The system’s detection capability enables expansion of multicolour panels and can keep pace with future fluorochrome development.
The ID7000 spectral cell analyser includes novel features, including the spectral reference library and the autofluorescence finder allowing researchers to identify and subtract contributions to the signal from autofluorescent populations, thus ensuring higher fidelity of data and more accurate visualization.
Spectral technology, easy-to-use software, and automation across the workflow make the ID7000 an excellent fit for facilities looking for a user-friendly, high-performance analyser that will bring their research to the next level.