Metabolomics with the Q Exactive

School of Biosciences - University of Birmingham
Event cost
Saturday 1 January 2022 (09:00-17:00)


Metabolomics with the Q Exactive

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Course Overview

This 3-day course introduces you to using the Q Exactive mass spectrometer in your metabolomics investigations. The course is led by experts in the field of metabolomics and includes lectures, laboratory sessions and computer workshops to provide a detailed overview of the metabolomics pipeline applying the Q Exactive mass spectrometer. 

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Topics Covered

  • Introduction to Metabolomics on the Q Exactive, the metabolomics workflow, and case studies using the Q Exactive
  • Using the Q Exactive family of instruments in your metabolomics investigations
  • Experimental design and the importance of quality control samples
  • Sample preparation including polar and non-polar preparation methods on biofluids (urine and plasma) and tissue samples
  • Preparation of samples for profiling and targeted analyses on the Q Exactive
  • Hands-on data acquisition for profiling and targeted studies, setting up the Vanquish UHPLC coupled to the Q Exactive MS
  • Data processing workshop
  • Data analysis workshop (univariate and multivariate analysis)
  • Introduction to metabolite identification applying Data Dependent Analysis
  • Question and answer session with a panel of experts
    • Tips and tricks
    • Problem solving

Preview the full course programme here



The course is aimed at individuals with minimal previous experience of applying LC-MS in metabolomics studies. A working knowledge of LC-MS would be advantageous.

Testimonials 2

Course Feedback

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of attendees said this course is excellent or good


of attendees would recommend this course to a colleague

"Very informative and well-structured course with friendly and enthusiastic tutors and plenty of opportunities to ask questions. I thoroughly enjoyed the course and came away with a lot of information and a better understanding of the metabolomics pipeline from experimental design through to data interrogation. Thank you for the great three days, I've learnt a lot."

"Thank you for a great course. The instructors were extremely knowledgeable and patient. Particular thanks to Cate and Andy who ran fantastic lab practical sessions."

"Very well structured lectures and helpful advice from experts!... Brilliantly structured introduction to metabolomics."


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See our Trainee Information Page here for more information, terms and conditions.


Please read the Birmingham Metabolomics Training Centre's Conditions and Cancellation Policy prior to registering.