Professor Iain Chapple PhD, BDS, FDSRCPS, FDSRCS, CCST (Rest Dent)

Iain Chapple

School of Dentistry
Head of the School of Dentistry
Professor of Periodontology and Consultant in Restorative Dentistry

Contact details

Address
The School of Dentistry
5 Mill Pool Way
Edgbaston
Birmingham
B5 7EG
United Kingdom

Iain Chapple is Head of the School of Dentistry within the Institute of Clinical Sciences. Iain is also Professor and Head of Periodontology within the School, and Honorary Consultant in Restorative Dentistry with Birmingham Community Health Trust. Iain has honorary consultant contracts with University Hospital Birmingham Foundation Trust and Birmingham Children’s Trust. Iain is former Designated Individual for the Human Tissue Authority in Dentistry at Birmingham.

Iain has published over 250 research papers and abstracts in scientific journals, and written and edited 7 textbooks and authored 16 book chapters in the field of Periodontology, Periodontal Medicine and Surgery, Nutrition and Molecular Methods. Iain has received major grants from the Medical Research Council, European Union and Industry. 

Iain is a committed an enthusiastic teacher of undergraduate and postgraduate students within Birmingham and also internationally on various graduate programs across Europe, North America and Asia-Pacific rim.

Iain is former Scientific Editor of the British Dental Journal, former Associate Editor of Journal of Periodontal Research, current Associate Editor of the Journal of Clinical Periodontology and Periodontology 2000. 

Iain was elected President of the International Association for Dental Research (IADR) Periodontal Research Group (PRG-2006-7), was Treasurer of the European Federation of Periodontology (EFP – 2007-2013) and is currently Secretary General of the EFP and Treasurer of the UK Oral and Dental Research Trust. He co-chairs the EFP workshops (research and education).

Iain is clinical lead for a hospital regional specialist periodontal service (referral base >6-million) and a national oral care service for adult Epidermolysis Bullosa patients.

Iain was awarded the Charles Tomes medal of the Royal College of Surgeons of England in 2012. 

Qualifications

  • Honorary fellow of the Royal College of Surgeons of Edinburgh (Dental Surgery) 2004.
  • Certificate of completion of specialist training (Rest Dent) – joint Royal Colleges 1996.
  • PhD (University of Birmingham) – 1993.
  • Fellow of the Royal College of Physicians and Surgeons of Glasgow (Dental Surgery) 1989.
  • Bachelor of Dental Surgery (Newcastle) 1986.

Biography

Iain Chapple qualified from Newcastle Dental School in 1986 and became House Surgeon until 1987, when Iain was appointed Senior House Officer in Maxillofacial Surgery at Sunderland’s District General Hospital. Here Iain obtained his pert-1 FDS of the Royal College of Surgeons and in 1988 Iain became Registrar at Leeds Dental Institute (Restorative Dentistry) and St James Hospital (Oral Surgery). Iain completed his Fellowship of the Royal College of Physicians and Surgeons of Glasgow in 1989 and moved to Birmingham as Clinical Lecturer in Restorative Dentistry in 1990. In 1993, Iain completed his PhD in developing enhanced chemiluminescence assays for biomarkers of periodontal inflammation and in 1996 Iain completed his certificate of Completion of Specialist Training in Restorative Dentistry from the Joint Royal Colleges.

Iain spent a post-doctoral period in 1995 developing chemiluminescence assays for oxidative stress at Harvard’s Forsyth Institute, Boston, USA and was appointed to Senior Lecturer and Honorary Consultant at the University of Birmingham in 1996 when Iain also became head of the Periodontal Department. Iain was appointed to his personal chair in 2001 and leads the Periodontal Research Group (PRG), part of Birmingham’s MRC Centre for Immune Regulation and the Centre for Ageing Research. Iain was awarded the Charles Tomes medal by the Royal College of Surgeons (2012), the Rizzo Research Award of the IADR PRG (2001). Iain won the British Society of Periodontology (BSP) Sir Wilfred Fish research prize (1993) and former and current PhD students (Roberts A, Brock, Milward, Ling, Sharma, White, Roberts H) also won this award. In 2010 (Dias) and 2011 (Palmer) post-doctoral fellows of the PRG won the Past Presidents Award of the IADR PRG. Various BSODR awards have been won by PhD students including Palmer, White and Sharma.

Iain established the clinical trials team at Birmingham’s Dental School in 1994, which runs clinical studies on disease pathogenesis as well as diagnosis and therapy to international GCP standards. The trials team allows for the translation of basic research by the group.

Iain has delivered annual keynote lectures/symposia at IADR, EuroPerio, and British Dental Association. The PRGs interests include:

  • Innate immunity in the pathobiology of periodontitis.
  • Neutrophil biology in chronic inflammatory diseases of ageing.
  • Diagnostic biomarkers for periodontitis and associated systemic diseases.
  • Periodontal inflammation and systemic inflammatory conditions.
  • Periodontitis and immune senescence.
  • Micro nutritional modulation of inflammation.
  • Clinical trials in periodontal therapy.

Teaching

  • BDS
  • BSc in Dental Hygiene and Therapy
  • MSc Advanced General Dental Practice
  • International programs (doctoral programs in Europe and USA – visiting Professor)

Postgraduate supervision

  • I Garner 1994-1998 - successful.
  • H Wight 1998-2002 - successful.
  • A Roberts 2000-2006 - successful.
  • F Murdoch 1999-2002 - successful.
  • G Brock 1999-2003 - successful.
  • M Milward 2003-2010 - successful.
  • L Palmer 2006-2010 - successful.
  • E Allen 2006-2010 - successful.
  • M Ling 2009-2015 – successful
  • S Kalra 2010-2014 - successful
  • P White 2011- 2015 - successful
  • H Roberts 2011-2016 - successful
  • S Serban 2011-2017 - successful
  • I Chicca 2012-current – EU FP7 ITN Phd studentship - successful
  • P Sharma 2013-current – NIHR Doctoral Fellow
  • J Batt 2012-current
  • Z Yonel 2015-current

Research


RESEARCH THEMES

      • Innate immunity, Clinical Trials, saliva/GCF biomarker analysis, microbiology, molecular nutrition.

RESEARCH ACTIVITY

Characterising the saliva and GCF proteome

As we age our bodies change and leave behind markers of these changes. Powerful profiling techniques such as “proteomics” can determine these changes. Recent studies have already identified changes between young and older women by analysing their saliva. This fluid contains many proteins that are involved in immunological protection as well as serum derived proteins. The group is currently employing highly accurate and sensitive mass spectrometry techniques such as FT-ICR MS/MS with i-TRAQ labelling to profile changes in the saliva proteome in healthy, diseased (gingivitis and periodontitis) and edentulous patients, as well as in gingival crevicular fluid (GCF). As the oral microflora changes with age, as well as with disease, using sensitive meta-proteomic techniques will help us to unravel biomarkers for healthy ageing and non-invasive techniques for disease diagnosis. Higher levels of biomarkers for dental caries (decay) associated proteins (Cystatin S) have already been found to be elevated in elderly patients with caries and many more discoveries are being made. Most recently, new peptides never before described in the periodontal environment have been identified, which provide insights into aspects of periodontal microanatomy and physiology never before contemplated. The development of biomarkers for early diagnosis of periodontitis and associated systemic diseases for near patient testing is a major focus.

Molecular nutrition & oxidative stress

Micronutrients can drive or resolve oxidative stress through various pathways including regulation of redox-sensitive gene transcription factors. A major interest of the PRG is in understanding how micronutrients control cell signaling pathways and in doing so regulate the inflammation. Clinical trials are also ongoing to evaluate novel therapeutic approaches through micronutrients applied systemically and topically and also to unravel pathogenic mechanisms in periodontitis.

Neutrophil extracellular trap formation

Recent reports of neutrophil hyper-reactivity in terms of ROS release and the involvement of IFNa in disease pathogenesis, have also, for the first time, raised the spectre of an autoimmune component to the pathogenesis of periodontitis. Moreover, type-1 interferon’s and ROS are associated with neutrophil extracellular trap (NET) formation. Whilst normally neutrophils combat periodontal pathogens by intracellular (phagocytosis) and extracellular (degranulation) mechanisms, NET killing has recently been described as an important new paradigm in neutrophil biology. NETs consist of a web of extracellular fibres of DNA plus histones (chromatin) and granular proteins, which bind to Gram-positive and Gram-negative bacteria. These chromatin structures immobilise high concentrations of extracellular antimicrobial peptides, including cathepsins and myeloperoxidase along with histones, leading to physical entrapment and killing of pathogenic bacteria. Lack of NET production (e.g. in Chronic Granulomatous Disease) or presence of bacteria that express DNAses which breakdown NETs, results in increased pathogenic activity and potentially catastrophic infections in certain patient subsets.  The DNA-histone backbone of NETs acts as a target for endogenous or bacterial peptidylarginine deiminases (PADs), which citrullinate histones as well as other proteins, by deimidating arginine residues, potentially rendering them auto-immunogenic. Further studies are currently ongoing in this area to determine whether this mechanism of generating auto-antigens links periodontitis to other age-related systemic diseases, such as rheumatoid arthritis, and whether decreased efficiency in NET production associates with immune-senescence.

Studying neutrophil behaviour

Evidence suggests that periodontitis occurs in individuals who have an abnormal neutrophil response to subgingival plaque bacteria and that neutrophil-mediated oxidant injury is an important feature of the disease. Peripheral blood neutrophils from periodontitis patients are hyper-reactive, after FcgR and toll-like receptor (TLR) stimulation, as well as being hyperactive in respect of unstimulated, extracellular release of reactive oxygen species (ROS).  Although FcgR hyper-reactivity is reduced by therapy, baseline, unstimulated-ROS release is not, suggesting that reactive and constitutive mechanisms underlie the “hyper-inflammatory” phenotype.  Priming with GM-CSF or the periodontal pathogens Porphyromonas gingivalis and Fusobacterium nucleatum (1 bacterial cell per neutrophil) reduces the FcgR-mediated hyper-reactivity but not hyperactive, baseline unstimulated ROS release.

Hyper-reactive neutrophils differentially express 25, type I interferon-stimulated genes and IFNa, which can enhance FcgR-mediated ROS generation in vitro, and is increased in plasma from periodontitis patients. Recent studies from this group have demonstrated that the ability of periodontitis plasma to prime for f-MLP-mediated ROS generation is, in part, due to the presence of GM-CSF, IL-8 and IFNa, supporting the idea that peripheral neutrophils in periodontitis patients are in a cytokine-primed state. Interestingly, transcripts for GM-CSF and IL-8 are up-regulated in gingival epithelial cells after stimulation with P. gingivalis and F. nucleatum, (see above), perhaps indicating that local tissue responses to plaque bacteria may contribute to peripheral neutrophil hyper-reactivity. There is currently, no data on the behaviour of PMNLs from older patients with respect to ROS release in response to different priming agents or to periodontal bacteria, and such studies are planned in order to shed light on this potentially important cause of periodontal tissue damage in older patients.

Analysing the oral epithelium

Epithelium was traditionally perceived as a simple inert physical barrier, whose role was to prevent microbial entry into the connective tissues, thereby protecting the host’s vital systems from the external environment. However, contemporary research has revealed that epithelial cells play an active role in the host response to bacterial infections. Gingival (gum) epithelium is intimately related to the bacterial plaque biofilm and research at the Birmingham Dental School has demonstrated that gingival epithelial stimulation by key periodontal pathogens results in activation of cellular transcription factors, which regulate production of pro-inflammatory cytokines. This finding suggests a key role for epithelium in the initiation and propagation of periodontal inflammation. However to date, research investigating how the responses and behaviour of oral epithelial cells change as we age, and thus impacts on these important mechanisms is almost completely lacking, and may help explain the increasing prevalence of periodontitis in older patients.

The Periodontal Research Group (PRG) at Birmingham proposes studies to explore age-related changes in oral epithelium in terms of:

      • Changes in efficiency of bacterial recognition (via pattern recognition receptors).
      • Activation of redox sensitive transcription factors (NF-kB, Nrf-2, & AP-1).
      • Gene transcription profiles.
      • Levels of cytokine production.
      • Levels of certain antioxidant micronutrients.
      • Changes in epithelial permeability and barrier function and how these may be manipulated using novel micronutrient approaches.

        The development of novel therapeutic approaches to periodontal therapy is a priority.

Other activities

  • Secretary General of European Federation of Periodontology.
  • Treasurer of UK Oral and Dental Research Trust.
  • Specialist advisor to Department of Health in Periodontology.
  • Board member of the European Nutraceutical Association.
  • Associate Editor of Journal of Clinical Periodontology.
  • Editorial Board member of Periodontology 2000.
  • Executive Committee member of European federation of Periodontology.
  • Global Expert Panel – Unilever Oral Care Research.
  • Expert advisor for Philips Oral Healthcare.
  • Expert advisor for Johnson & Johnson.
  • Consultant to PreViser.co.uk – spin out company of The University of Birmingham.
  • Executive Board member of European Nutraceutical Association.

Publications

Ten Most Significant Manuscripts:

172.   Roberts H, White P, Dias I, McKaig S, Veeramachaneni R, Thakker N, Grant M, Chapple I. Characterisation of neutrophil function in Papillon-Lefevre Syndrome. J Leukocyte Biology. 2016: 100; 433-444. doi:10.1189/jlb.5A1015-489R.

171.   Spengler J, Lugonja B, Ytterberg AJ, Zubarev RA, Creese A, Pearson M, Grant M, Milward M, Lundberg K, Buckley C, Filer A, Raza K, Cooper P, Chapple ILC, Scheel-Toellner D. Release of active peptidyl arginine deiminases by neutrophils can explain production of extracellular citrullinated autoantigens in RA synovial fluid. Arthritis & Rheumatol 2015: 67; 3135-3145.

170.   P de Pablo, T Dietrich, ILC Chapple, M Milward, M Chowdhury, Charles PJ, Buckley CD, Venables PJ. The autoantibody repertoire in periodontitis: a role in the induction of autoimmunity to citrullinated proteins in rheumatoud arthritis? Ann Rheum Dis. 2014: 73; 590-586.

169.  Hazeldine J, Harris P, Chapple IL, Grant M, Greenwood H, Livesey A, Sapey E, Lord JM. Impaired neutrophil extracellular trap formation: a novel defect in the innate immune system of aged individuals. Aging Cell. 2014: 13; 690-698. doi: 10.1111/acel.12222. Epub 2014 Apr 30.

168.   Dias IHK, Chapple ILC, Milward M, Grant MM, Hill E, Brown J, Griffiths HR. Sulforaphane Restores Cellular Glutathione Levels and Reduces Chronic Periodontitis Neutrophil Hyperactivity In Vitro. PLoS ONE 2013.8(6): e66407. doi:10.1371/journal.pone.0066407.

167.   Palmer L, Cooper PR, Ling M, Huissoon A, Chapple ILC. Hypochlorous acid regulates neutrophil extracellular trap formation. Clin Exp Immunol 2012: 167; 261-268. doi:1111/j.1365-2249.2011.04518.x.

166.   de Pablo P, Chapple ILC, Buckley CD, Dietrich TD. Periodontitis in systemic rheumatic diseases. Nature Rev. Rheumatol. 2009: 5; 218-224.

165.   Dias IH, Marshall L, Chapple ILC, Lambert PA, Matthews JB, Griffiths HR. Gingipains from Porphyromonas gingivalis increase the chemotactic and respiratory burst-priming properties of the 77-amino-acid interleukin-8 variant. Infection & Immunity 2008: 761; 317-323.

164.   Wright HJ, Matthews JB, Chapple ILC, Ling-Mountford N, Cooper PR. Periodontitis associates with a type 1 IFN signature in peripheral blood neutrophils. J Immunol. 2008: 181; 5775-5784.

163.   Matthews JB, Wright HJ, Roberts A, Cooper PR, Chapple ILC. Hyperactivity and reactivity of peripheral blood neutrophils in chronic periodontitis. Clin Exp Immunol, 2007: 147; 255-264.

162.   Milward MR, Chapple ILC, Wright HJ ,Millard JL, Matthews JB, Cooper P. Differential activation of NF-kB and gene expression in oral epithelial cells by periodontal pathogens. Clin Exp Immunol. 2007: 148; 307-324.

Scientific Papers in Peer Reviewed Literature last 5-years:

161.   Gorecki P, Burke DL, Chapple ILC, Hemming K, Saund D, Pearson D, Stahl W, Lellog R, Dietrich T. Perioperative supplementation with a fruit and vegetable juice powder concentrate and 1 postsurgical morbidity: a double-blind, randomised, placebo controlled clinical trial. Clinical Nutrition 2017 (in press).

160.   Busby M, Fayle S, Chapple L, Clover H, Chapple IL. Practitioner evaluation of an online oral health and risk assessment tool for young patients. Brit Dent J 2017 (in press).

159.   Chapple ILC, Griffiths HR, Milward MR, Ling MR, Grant MM. Antioxidant micronutrients and oxidative stress biomarkers. Methods Molec Biol 2017: 1537; 61-77.

158.   Milward MR, Ling MR, Grant MM, Chapple ILC. Oral epithelial cell culture model for studying the pathogenesis of chronic inflammatory disease. Methods Molec Biol 2017: 1537; 381-401.

157.   White PC, Chicca IJ, Ling MR, Wright HJ, Cooper PR, Milward MR, Chapple ILC. Methods Molec Biol 2017: 1537; 481-497.

156.   Chapple ILC, Bouchard P, Cagetti MG, Campus G, Carra MC, Cocco F, Nibali L, Hujoel P, Laine ML, Lingstrom P, Manton DJ, Montero E, Pitts N, Rangé H, Schlueter N, Teughels W, Twetman S, Van Loveren C, Van der Weijden F, Vieira AR, Schulte AG. Interaction of lifestyle, behaviour or systemic diseases with dental caries and periodontal diseases: consensus report of group 2 of the joint EFP/ORCA workshop on the boundaries between caries and periodontal diseases. J Clin Periodontol 2017: 44(s18); 39-51.

155.   Binder V, Bergum B, Jaisson S, Gillery P, Scavenius C, Spriet E, Nyhaug AK, Roberts HM, Chapple ILC, Hellvard A, Delaleu N, Mydel P. Impact of fibrinogen carbamylation on fibrin clot formation and stability. Thromb Haemost 2017: 117; 899-910.

154.   Hobbins S, Chapple ILC, Sapey E, Stockley RA. Is periodontitis a co-morbidity of COPD or can associations be explained by shared risk factors/behaviours? Int J Chronic Obstruct Dis. 2017: 12 ; 1339-1349.

153.   Kilian M, Chapple ILC, Hanig M, Marsh PD, Pedersen AM, Tonetti MS, Wade WG, Zaura E. The oral microbiome, an update for oral healthcare professionals. British Dent J, 2016: 221 ; 657-666.

152.   Roberts HM, Ling MR, Insall R, Kalna G, Spengler J, Grant MM, Chapple IL. Impaired neutrophil directional chemotactic accuracy in chronic periodontitis patients. J Clin Periodontol. 2015: 42; 1-11. doi: 10.1111/jcpe.12326. Epub 2015 Jan 8.

151.   White, P., Sakellari, D., Roberts, H., Risafi, I., Ling, M., Cooper, P., Milward, M., Chapple, ILC. Peripheral Blood Neutrophil Extracellular Trap Production and Degradation in Chronic Periodontitis. J Clin Periodontol. 2016: 43 ; 1041-1049.

150.   Hirschfeld J, Roberts HM, Chapple ILC, Parcina M, Jepsen S, Johansson A, Claesson A. Effects of Aggregatibacter actinomycetemcomitans leukotoxin on neutrophil migration and extracellular trap formation. J Oral Microbiology, 2016: 8 ; 33070.

149.   Ling MR, Chapple ILC, Matthews JB. Neutrophil superoxide release and plasma C-reactive protein levels pre- and post-periodontal therapy. J Clin Periodontol. 2016 : 43 ; 652-658.

148.   Sharma P, Busby M, Chapple E, Matthews R, Chapple IL. The relationship between general health and lifestyle factors and oral health outcomes. British Dent J 2016: 221; 65-69.

147.   Davis IJ, Jones AW, Creese AJ, Staunton R, Atwall J, Chapple ILC, Harris S, Grant MM. Longitudinal quantification of the GCF proteome during progression from gingivitis to periodontitis in a canine model. J Clin Periodontol. 2016 2016 Mar 17. doi: 10.1111/jcpe.12548

146.   Sharma P, Chapple I, Cockwell P, Dietrich T. INfluence of Successful Periodontal Intervention in Renal Disease (INSPIRED): A randomised controlled feasibility clinical trial. Trials. 2017 (in press).

145.   Ling MR, Chapple ILC, Creese AJ, Matthews JB. Effects of C-reactive protein on the neutrophil respiratory burst in vitro. Innate Immunity 2014: 20; 339-349. doi: 10.11771753425913493199.

144.   Koro C, Hellvard A, Delaleu N, Binder V, Scavenius C, Bergum B, Główczyk I, Roberts HM, Chapple IL, Grant MM, Rapala-Kozik M, Klaga K, Enghild JJ, Potempa J, Mydel P. Carbamylated LL-37 as a modulator of the immune response. Innate Immunity. 2016 : 22 ; 218-229.

143.   Ling MR, Chapple ILC, Matthews JB. Peripheral blood neutrophil cytokine hyperreactivity in chronic periodontitis. Innate Immunity 2015: DOI: 10.1177/1753425915589387.

142.   Sharma P, Dietrich T, Ferro CJ, Cockwell P, Chapple IL. Association between Periodontitis and mortality in stages 3-5 Chronic Kidney Disease: NHANES III and linked mortality study. J Clin Periodontol. 2016 43, 104-113. Dec 31. doi: 10.1111/jcpe.12502. [Epub ahead of print].

141.   Lugonja B, Yeo L, Milward MR, Smith D, Dietrich T, Chapple IL, Rauz S, Williams GP, Barone F, de Pablo P, Buckley C, Hamburger J, Richards A, Poveda-Gallego A, Scheel-Toellner D, Bowman SJ. Periodontitis prevalence and serum antibody reactivity to periodontal bacteria in primary Sjögren’s syndrome. J Clin Periodontol. 2016 : 43 ; 26-33. doi: 10.1111/jcpe.12485.

140.   Yonel Z, Parma S, Chapple IL. Juvenile Hyaline Fibromatosis: Impact of Periodontal Care on Quality of Life and a Patient Perspective. Dent Update. 2015 : 42 ; 656-8, 660-2, 665-6

139.   White PC, Chicca IJ, Cooper PR, Milward MR, Chapple IL. Neutrophil Extracellular Traps in Periodontitis: A Web of Intrigue. J Dent Res. 2016: 95; 26-34. doi: 10.1177/0022034515609097.

138.   Meyle J, Chapple IL.Molecular aspects of the pathogenesis of periodontitis. Periodontology 2000. 2015: 69 ; 1):7-17. doi: 10.1111/prd.12104.

137.   Spengler J, Lugonja B, Jimmy Ytterberg A, Zubarev RA, Creese AJ, Pearson MJ, Grant MM, Milward M, Lundberg K, Buckley CD, Filer A, Raza K, Cooper PR, Chapple IL, Scheel-Toellner D. Release of Active Peptidyl Arginine Deiminases by Neutrophils Can Explain Production of Extracellular Citrullinated Autoantigens in Rheumatoid Arthritis Synovial Fluid. Arthritis Rheumatol. 2015 : 67 ; 3135-3145. doi: 10.1002/art.39313.

136.   White P, Cooper P, Milward M, Chapple I. Differential activation of neutrophil extracellular traps by specific periodontal bacteria. Free Radic Biol Med. 2014: 75(s1:s53). doi: 10.1016/j.freeradbiomed.2014.10.827.

135.   DePablo P, Dietrich T, Chapple ILC, Milward M, Buckley CD, Venables PJ. Response to: “The autoantibody repertoire in periodontitis: a role in the induction of autoimmunity to citrullinated proteins in rheumatoud arthritis? Antibodies against uncitrullinated peptides seem to occur prior to the antibodies to the corresponding citrullinated peptides” by Brink et al. Ann Rheum Dis. 2014;0:1

134.   Blair FM, Chapple ILC. Prescribing for periodontal disease. Prim Dent J. 2014 : 3 ; 38-43. doi: 10.1308/205016814813877234.

133.   Chapple IL, Van der Weijden F, Doerfer C, Herrera D, Shapira L, Polak D, Madianos P, Louropoulou A, Machtei E, Donos N, Greenwell H, Van Winkelhoff AJ, Eren Kuru B, Arweiler N, Teughels W, Aimetti M, Molina A, Montero E, Graziani F. Primary prevention of periodontitis: managing gingivitis. J Clin Periodontol. 2015 : 42(s16) ; 71-76. Doi : 10.1111/jcpe.12382.

132.  Sculean A, Nikolidakis D, Nikou G, Ivanovic A, Chapple IL, Stavropoulos A. Biomaterials for promoting periodontal regeneration in human intrabony defects: a systematic review. Periodontol 2000. 2015: 68; 182-216. doi: 10.1111/prd.12086.

131.   Sculean A, Chapple IL, Giannobile WV. Wound models for periodontal and bone regeneration: the role of biologic research. Periodontol 2000. 2015: 68; 7-20. doi: 10.1111/prd.12091.

130.   Tonetti MS, Chapple IL, Jepsen S, Sanz M. Primary and secondary prevention of periodontal and peri-implant diseases: Introduction to, and objectives of the 11th European Workshop on Periodontology consensus conference. J Clin Periodontol. 2015: 42; S1-4. doi: 10.1111/jcpe.12382.

129.   Chapple IL, Van der Weijden F, Doerfer C, Herrera D, Shapira L, Polak D, Madianos P, Louropoulou A, Machtei E, Donos N, Greenwell H, Van Winkelhoff AJ, Eren Kuru B, Arweiler N, Teughels W, Aimetti M, Molina A, Montero E, Graziani F. Primary prevention of periodontitis: managing gingivitis. J Clin Periodontol. 2015: 42; S71-6. doi: 10.1111/jcpe.12366.

128.   Borgnakke WS, Chapple IL, Genco RJ, Armitage G, Bartold PM, D'Aiuto F, Eke PI, Giannobile WV, Kocher T, Kornman KS, Lang NP, Madianos PN, Murakami S, Nishimura F, Offenbacher S, Preshaw PM, Rahman AU, Sanz M, Slots J, Tonetti MS, Van Dyke TE. J Ev Based Dent.2014: 14; 127-132. Doi: 10.1016/j.jebdp.2014.04.017.

127.   Chapple IL, Borgnakke WS, Genco RJ. Hemoglobin A1c levels among patients with diabetes receiving nonsurgical periodontal treatment. JAMA. 2014: 311;1919-20. doi: 10.1001/jama.2014.2228. No abstract available.

126.   Sharma P, Dietrich T, Sidhu A, Vithlani V, Rahman M, Stringer S, Jesky M, Kaur O, Ferro C, Cockwell P, Chapple IL. The periodontal health component of the Renal Impairment In Secondary Care (RIISC) cohort study: a description of the rationale, methodology and initial baseline results. J Clin Periodontol. 2014: 41; 653-61. doi: 10.1111/jcpe.12263.

125.   Chapple ILC. Time to take periodontitis seriously. The benefits of treatment are likely to exceed the costs. Brit Med Journal. 2014:348:g2645

124.  Busby M, Martin JA, Matthews R, Burke FJ, Chapple I. The relationship between oral health risk and disease status and age, and the significance for general dental practice funding by capitation. Br Dent J. 2014: 217;E19. doi: 10.1038/sj.bdj.2014.1006

123.  Busby M, Chapple L, Matthews R, Burke FJ, Chapple I. Continuing development of an oral health score for clinical audit. Br Dent J. 2014: 216; E20. doi: 10.1038/sj.bdj.2014.352

122.   Chapple ILC, Wilson N. Chronic non-communicable diseases. Br Dent J. 2014: 216;357. Doi: 10.1038/sj.bdj.2014.357.

121.   Chapple ILC, Busby M, Clover H, Matthews R. Periodontal disease and systemic disease. J Royal Soc Med. 2014: 107(3): 94. DOI: 10.1177/0141076813518770

120.  Jones AW, Grant MM, Cooper HJ, Chapple ILC. The Presence of 3-Nitrotyrosine Affects the Site of Nitrosylation. Free Radical Biology and Medicine. DOI:10.1016/j.freeradbiomed. 2013.10.611

119.   Chapple IL, Matthews JB, Wright HJ, Scott AE, Griffiths HR, Grant MM. Ascorbate and α-tocopherol differentially modulate reactive oxygen species generation by neutrophils in response to FcγR and TLR agonists. Innate Immunity. 2013;19(2):152-9. doi: 10.1177/1753425912455207.

118.   Milward MR, Chapple IL, Carter K, Matthews JB, Cooper PR. Micronutrient modulation of NF-κB in oral keratinocytes exposed to periodontal bacteria. Innate Immunity. 2013: 19; 140-51. doi: 10.1177/1753425912454761.

117.   Stringer S, Sharma P, Dutton M, Jesky M, Ng K, Kaur O, Chapple I, Dietrich T, Ferro C, Cockwell P. The natural history of, and risk factors for, progressive chronic kidney disease (CKD): the Renal Impairment in Secondary care (RIISC) study; rationale and protocol. BMC Nephrol. 2013: 25; 14:95. doi: 10.1186/1471-2369-14-95.

116.   Cooper PR, Palmer LJ, Chapple IL. Neutrophil extracellular traps as a new paradigm in innate immunity: friend or foe? Periodontol 2000. 2013: 63; 165-97. doi: 10.1111/prd.12025.

115.   Busby M, Chapple E, Matthews R, Chapple IL. Practitioner evaluation of a novel online integrated oral health and risk assessment tool: a practice pilot. Br Dent J. 2013: 215; 115-20. doi: 10.1038/sj.bdj.2013.738. Erratum in: Br Dent J. 2013 Sep;215(5):212.

114.   Borgnakke WS, Chapple IL, Genco RJ, Armitage G, Bartold PM, D'Aiuto F, Eke PI, Giannobile WV, Kocher T, Kornman KS, Lang NP, Madianos PN, Murakami S, Nishimura F, Offenbacher S, Preshaw PM, Rahman AU, Sanz M, Slots J, Tonetti MS, Van Dyke TE. The multi-center randomized controlled trial (RCT) published by the journal of the American Medical Association (JAMA) on the effect of periodontal therapy on glycated hemoglobin (HbA1c) has fundamental problems. J Evid Based Dent Pract. 2014: 14:127-32. doi: 10.1016/j.jebdp.2014.04.017.

113.   Chapple IL, Genco R; Working group 2 of joint EFP/AAP workshop. Diabetes and periodontal diseases: consensus report of the Joint EFP/AAP Workshop on Periodontitis and Systemic Diseases. J Clin Periodontol. 2013: 40; S106-12. doi: 10.1111/jcpe.12077.

112.   Matthews JB, Chen F-M, Milward MR, Ling M, Chapple ILC. Neutrophil superoxide production in the presence of cigarette smoke extract, nicotine and cotinine. J Clin Periodontol 2012: 39(7):626-34. doi: 10.1111/j.1600-051X.2012.01894.x.

111.   Grant MM, Wilson J, Rock PR, Chapple ILC. Biomarkers in GCF from patients undergoing orthodontic treatment. Brit J Orthod 2012: 35; 644-651.

110.   Scott A, Milward MR, Brock GR, Carlile M, Linden G, Lundy F, Naeeni MA, Matthews JB, Kinane DF, Chapple ILC. Mapping biological to clinical pheonotypes during the development (21 days) and resolution (21 days) of experimental gingivitis. J Clin Periodontol 2012; 39: 123–131. doi: 10.1111/j.1600-051X.2011.01825.x.

109.   Chapple ILC, Milward MR, Ling-Mountford N, Weston P, Carter K, Askey K, Dallal GE, DeSpirt S,  Sies H, Patel D, Matthews JB. Adjunctive daily supplementation with encapsulated fruit, vegetable and berry juice powder concentrates and clinical periodontal outcomes: a double blind RCT. J Clin Periodontol 2012;39:62-72. doi: 10.1111/j.1600-051X.2011.01793.x.

108.   Sanz M, Chapple IL. Clinical research on peri-implant diseases: consensus report of Working Group 4. Working Group 4 of the VIII European Workshop on Periodontology. J Clin Periodontol. 2012: 39 (S12) ;202-206. doi: 10.1111/j.1600-051X.2011.01837.x.

107.   Chapple ILC, Milward MR, Ling-Mountford N, Weston P, Carter K, Askey K, Dallal GE, DeSpirt S, Sies H, Pate D, Matthews JB. Adjunctive daily supplementation with encapsulated fruit, vegetable and berry juice powder concentrates and clinical periodontal outcomes: a double blind RCT. J Clin Periodontol 2012: 39; 62-71 (doi: 10.1111/j.1600-051X.2011.01793.x.).

Scientific Papers in Peer Reviewed Literature prior to last 5-years:

106.   Palmer L, Chapple ILC, Wright HJ, Roberts A, Cooper PR. Extracellular DNAse production by periodontal bacteria. J Perio Res 2011: 47; 439-445. doi:10.1111/j.1600-0765.2011.01451.x

105.   Allen EA, Matthews JB, O’Halloran D, Griffiths H, Chapple ILC. Oxidative and Inflammatory status in Type 2 Diabetes Patients with Periodontitis. J Clin Periodontol 2011: 38; 894-901. doi: 10.1111/j.1600-051X.2011.01764.x.

104.   Creese A, Grant MM, Chapple ILC, Cooper HR. On-line liquid chromatography neutral loss-triggered electron transfer dissociation mass spectrometry for the analysis of citrullinated peptides. Analytical Methods 2011: 3; 259-266.

103.   Wright HJ, Chapple ILC, Matthews JB, Cooper PR. Fusobacterium nucleatum regulation of neutrophil transcription. J Periodont Res 2011: 46; 1-12.

102.   Monksfield P, Chapple ILC, Matthews JB, Grant MM, Addison O, Reid AP, Proops DW, Sammons RL. Biofilm formation on bone anchored hearing aids. The Journal of Laryngology and Otology. 2011: 125; 1125-1230 (doi:10.1017/S0022215111002143).

101.   Palmer L, Chapple ILC, Roberts A, Cooper PR. Extracellular DNAse production by periodontal bacteria. J Periodontal Research 2011: 47; 439-454.

100.   Scott A, Milward MR, Brock GR, Carlile M, Linden G, Lundy F, Naeeni MA, Matthews JB, Kinane DF, Chapple ILC. Mapping biological to clinical pheonotypes during the developent (21 days) and resolution (21 days) of experimental gingivitis. J Clin Periodontol 2011: 39; 123-131.

99.     Allen EA, Matthews JB, O’Halloran D, Griffiths H, Chapple ILC. Oxidative and Inflammatory status in Type 2 Diabetes Patients with Periodontitis. J Clin Periodontol 2011: 38; 894-901. (doi: 10.1111/j.1600-051X.2011.01764.x).

98.     Van der Velden U, Kuzmanova D, Chapple ILC. Micronutritional approaches to periodontal therapy. J Clin Periodontol 2011: 38(s11); 142-158.

97.     Tonetti, M.S., Chapple, I.L.C. Biological approaches to the development of novel periodontal therapies – Consensus of the Seventh European Workshop on Periodontology. J Clin Periodontol 2011: 38(s11), 114-118.

96.     Sanz, M., Lang, N.P., Kinane, D.F., Berglundh, T., Chapple, I.L.C., Tonetti, M. Seventh European Workshop on Periodontology of the Euroeapn Academy of Periodontology at the Parador at La Granja, Segovia, Spain. J Clin Periodontol 2011: 38(s11), 1-2.

95.     Creese A, Grant MM, Chapple ILC, Cooper HR. On-line liquid chromatography neutral loss-triggered electron transfer dissociation mass spectrometry for the analysis of citrullinated peptides. Analytical Methods 2011: 3; 259-266.

94.     Wright HJ, Chapple ILC, Matthews PR, Cooper PR. Fusobacterium nucleatum regulation of neutrophil transcription. J Periodont Research 2011: 46; 1-12.

93.     Matthews JB, Chen F-M, Milward MR, Wright HJ, Carter K, McDonagh A, Chapple ILC. Effect of nicotine, cotinine and cigarette smoke extract on the neutrophil respiratory burst. J Clin Periodontol 2011: 38; 208-213.

92.     Grant MM, Creese A, Barr G, Ling M, Matthews JB, Chapple ILC. Proteomic analysis of a non-invasive human model of acute inflammation and its resolution: the 21 day gingivitis model. J Proteome Res. 2010: 9; 4732-4744.

91.     Grant MM, Brock GR, Matthews JB, Chapple ILC. Crevicular fluid glutathione levels in periodontitis and the effect of non-surgical therapy. J Clin Periodontol. 2010: 37; 17-23.

90.     Grant MM, Kolamunne RT, Locke FE, Matthews JB, Chapple ILC, Griffiths HR. Oxygen tension modulates the cytokine response of oral epithelium to periodontal bacteria. J Clin Periodontol 2010: 37; 1039-1048.

89.     Dias HK, Wright HJ, Chapple ILC, Matthews JB, Griffiths HR. Activation of the neutrophil respiratory burst by plasma from periodontitis patients is mediated by pro-inflammatory cytokines. J Clin Periodontol 2010: 38; 1-7.

88.     Chapple ILC. Nutritional control of periodontal inflammation. Proc Nutr Metab 2010: 59-70.

87.     Grant MMMonksfield P, Proops D, Brine M, Addison O, Sammons RL, Matthews JB, Reid A, Chapple ILC. Fluid exudates from inflamed bone-anchored hearing aids demonstrate elevated levels of cytokines and biomarkers of tissue and bone metabolism. Otol Neurotol. 2010: 31;433-439.

86.     Mattheos N, Schoonheim-Klein M, Walmsley AD, Chapple ILC. Innovative educational methods and technologies applicable to continuing professional development in periodontology. Eur J Dent Educ. 2010: 14 (S1);43-52.

85.     Chapple ILC, Walmsley AD, Schoonheim-Klein M, Mattheos N. Conclusions and consensus statements on: Innovative educational methods and technologies applicable to continuing professional development in periodontology – consensus view 4. Consensus report of the 1st European Workshop on Periodontal Education. Eur J Dent Educ. 2010: 14 (S1);41-42.

84.     Sanz M, Chapple ILC. First European consensus workshop in periodontal education – objectives and overall recommendation. Eur J Dent Educ. 2010: 14 (S1);1.

83.     Chapple ILC. Perioodntal diagnosis and treatment: where does the future lie? Periodontol 2000. 2009: 51; 9-21.

82.     Hutton A, Bradwell M, English M, Chapple ILC. The oral health needs of children after treatment for a solid tumour or lymphoma. Int J Paed Dent. 2010: 20; 15-23.

80.     Allen EM, Matthews JB, O'Connor R, O'Halloran D, Chapple ILC. Periodontitis and type 2 diabetes: is oxidative stress the mechanistic link? Scottish Medical Journal. 2009:542;41-7.

79.     Chapple ILC. The impact of oral disease on systemic health - What is the evidence and how big is the problem? Summary and conclusions - Where now? J Dentistry. 2009:378;S583-84

78.     Chapple ILC. The impact of oral disease upon systemic health - symposium overview.J Dentistry. 2009: 378; S568-S571.

77.     Chen F-M,Shelton RM,Jin Y,Chapple ILC. Localized delivery of growth factors for periodontal tissue regeneration: role, strategies and perspectives. Med Res Rev.  2009: 293; 472-513.

76.     Chapple ILC. Potential mechanisms underpinning the nutritional modulation of periodontal inflammation. JADA. 2009: 1402; 178-184.

75.     Chapple ILC, Hill KB. Getting the message across to periodontitis patients: the role of personalised biofeedback. Int Dent J.  2008: 585; 294-306.

74.     Matthews JB, Wright HJ, Roberts A, Ling-Mountford N, Cooper P, Chapple ILC. Neutrophil Hyper-responsiveness in Periodontitis. J Dental Research. 2007: 886; 718-722.

73.     Chapple ILC, Milward MR, Dietrich T. The prevalence of inflammatory periodontitis is negatively associated with serum antioxidant concentrations. J Nutrition. 2007: 137; 657-664.

72.     Chapple ILC, Matthews JB. The role of reactive oxygen and antioxidant species in periodontal tissue destruction. Periodontology 2000 2007: 43; 160-232.

71.     Chapple ILC, Brock GR, Milward MR, Ling N, Matthews JB. Compromised GCF Total Antioxidant Capacity in Periodontitis - Cause or Effect? J Clin Periodontol 2007:34;103-10.

70.     Chapple ILC. Oxidative stress, nutrition and neutrogenomics in periodontal health and disease. Int J Dent Hyg. 2006: 4 (suppl 1); 15-21.

69.     Maxwell SRJ, Dietrich T, Chapple ILC. The prediction of total antioxidant activity from the concentration of individual serum antioxidants. Clin Chim Acta. 2006: 372; 188-194.

68.     Wright H, Chapple ILC, Cooper PR, Matthews JB. Platelet-derived growth factor (PDGF) isoform and PDGF receptor expression in drug-induced gingival overgrowth and hereditary gingival fibrosis. Oral Diseases. 2006: 12; 315-323.

67.     Barnfather KDP, Cope GF, Chapple I.L.C. Randomised controlled trial investigating the impact of incorporating a 10-minute point of care test for salivary nicotine metabolites into a general practice-based smoking cessation programme. British Medical Journal. 2005: 331; 869-872 doi:10.1136/bmj.38621.463900.7C.

66.     Roberts A, Matthews JB, Socransky SS, Freestone P, Williams PH, Chapple ILC. Stress and the periodontal diseases: growth responses of periodontal bacteria  to Escherichia coli stress-associated autoinducer and exogenous Fe. Oral Microbiol & Immunol. 2005: 20; 147-153.

65.     Mainnemare A, Mégarbane B, Soueidan A, Daniel A, Chapple I.L.C. Hypochlorous acid and taurine-N-monochloramine in periodontal diseases: hypotheses for a novel therapeutic approach? J Dental Research. 2004: 83; 823-831.

64.     Brock G, Matthews JB, Butterworth CJ, Chapple ILC. Local and systemic  antioxidant capacity in periodontitis health. J Clin Periodontol. 2004: 31; 515-521.

63.     Murdoch FE, Sammons R, Chapple ILC. Isolation and categorisation of subgingival staphylococci from periodontitis patients and controls:  implications for the pathogenesis of prosthetic valve endocarditis. Oral Diseases. 2004: 10; 155-162.

62.     Wright HJ, Chapple ILC, Blair F, Matthews JB.           Crevicular fluid levels of TGFbeta1 in drug-induced gingival overgrowth. Archives of Oral Biology. 2004: 49; 421-425.

61.     Roberts A, Matthews JB, Socransky SS, Freestone PPE, Williams PH, Chapple ILC. Stress and the periodontal diseases: effects of catecholamines on the growth of periodontal bacteria in vitro. Oral Microbiol & Immunol. 2003: 17; 296-303.

60.     Wright HJ, Chapple ILC, Matthews JB. Levels of TGFbeta1 in gingival crevicular fluid during a 21-day experimental model of gingivitis. Oral Diseases. 2003: 9; 1-7.

59.     Chapple ILC, Roberts A, Shah M. C-1 esterase inhibitor dysfunction localised to the periodontal tissues: clues to the role of stress in the pathogenesis of chronic periodontitis. J Clin Periodontol. 2003: 30; 271-277.

58.     Brock G R, Matthews JB, Chapple ILC, Harding CR. Peripheral and local antioxidant defence in periodontal disease and health, by enhanced chemiluminescence. In Bioluminescence & Chemiluminescence - Current Applications. Ed P Stanley, LJ Kricka.  World World Scientific. 2003: 241-244.

57.     Chapple ILC, Brock G, Eftimiadi C, Matthews JB. Glutathione in gingival crevicular fluid and its relation to local antioxidant capacity in periodontal health and disease.  J Clin Pathol: Molec Pathol. 2002: 78; 367-373.

56.     McCracken GI, Chapple ILC, Stacey F, Heasman L, Milward MR, Heasman PA. Efficacy of a prototype brush head for a powered toothbrush: a multi-centre study.  J Clin Periodontol. 2002: 29; 889-895.

55.     Wright HJ, Chapple ILC, Matthews JB. TGF-beta isoforms and TGF-beta receptors in drug-induced and hereditary gingival overgrowth. J Oral Pathol & Medicine. 2001: 30; 281-289.

54.     Hennig BJW, Parkhill JM, Chapple ILC, Heasman PA, Taylor JJ.  Dinucleotide repeat polymorphism in the interleukin-10 gene promoter (IL-10 G) and genetic susceptibility to early-onset periodontal disease. Genes & Immunity. 2000: 1; 402-404.

53.     Nayar P, Cope G, Brock G, Chapple ILC. A near patient test for measuring salivary cotinine levels. Ann Clin Biochem. 2000: 37; 666-673.

52.     Wright HJ, Chapple ILC, Matthews JB. TGFB isoforms and TGFB receptors in drug-induced and hereditary gingival overgrowth. J Oral Pathol & Medicine. 2001: 30; 281-290.

51.     Parkhill JN, Hennig BJW, Chapple ILC, Heasman PA, Taylor JJ. Association of IL-1 gene polymorphisms and early-onset periodontitis. J Clin Periodontol. 2000: 27; 682-689.

50.     Chapple ILC, Hamburger J. The significance of oral health in HIV disease. J  Sexual Trans Infect. 2000: 76; 236-243.

49.     Chapple ILC, Saxby MS, Murray J. Gingival haemorrhage, myelodysplastic syndromes and acute myeloid leukaemia. J Periodontol. 1999: 70; 1247-1253.

48.     Hennig BJW, Parkhill JN, Chapple ILC, Heasman PA, Taylor JJ. Association of vitamin D receptor gene polymorphism with localised early-onset periodontal disease. J Periodontol. 1999: 70; 1032-1038.

47.     Chapple ILC, Landini G, Griffiths GS, Patel NC, Ward RSN. Calibration of the Periotron 8000  and 6000  by polynomial regression. J Periodont Research. 1999: 34; 79-86.

46.     Chapple ILC, Garner I, Saxby MS, Moscrop HM, Matthews JB. Predication and diagnosis of attachment loss by enhanced chemiluminescent assay of crevicular fluid alkaline phosphotate levels. J Clin Periodontol. 1999: 26; 190-198.

45.     Dibart S, Chapple ILC, Skobe Z, Shusterman S, Needleman HL. Microbiological findings in prepubertal periodontitis. J Periodontol. 1998: 69; 1172-1175.

44.     Chapple ILC. Periodontal disease, free radicals and antioxidants. The Experts Speak - Hamilton K & Simpson K (Eds) - The Role of Nutrition in Medicine. 1997: 106-107.

43.     Chapple ILC. Free radicals, reactive oxygen species and antioxident defence in the aetiology of the periodontal diseases. The Bulletin of the Royal College of Physicians and Surgeons Glas. 1997: 26; 22-24.

42.     Chapple ILC, Mason GM, Matthews JB, Thorpe GHG, Maxwell SRJ, Whitehead T. Enhanced chemiluminescent assay for measuring the total antioxidant capacity of serum, saliva and crevicular fluid. Ann Clin Biochem. 1997: 34; 412-421.

41.     Chapple ILC. Periodontal diseases in children and adolescents: classification, aetiology and management. J Oral Health. 1996: 86; 7-16.

40.     Chapple ILC, Thorpe GHG, Mason GI, Matthews JB. Low molecular weight thiol antioxidant activity in oral fluids.     Bioluminescence & Chemiluminescence - Szalay A, Kricka L J & Stanley P (Eds). 1997: 485-488.

39.     Chapple ILC. Reactive oxygen species and antioxidants in inflammatory diseases. J Clin Periodontol. 1997: 24; 287-296.

38.     Chapple ILC. The role of free radicals and antioxidants in the pathogenesis of the inflammatory periodontal diseases. J Clin Molec Pathol. 1996: 49; M247-M255 .

37.     Chapple ILC. Periodontal disease diagnosis: current status and future developments. J Dentistry. 1997: 25; 3-15.

36.     Chapple ILC. The pathogenesis of periodontal disease. The Periodontium and Orthodontics in Health and Disease - Heasman PA, Millett DT, ChappleIL. (Eds): OxfordUniversity Press, 1996: 2; 33-72.

35.     Chapple ILC, Socransky SS, Dibart SN, Glenwright HD, Matthews JB. Chemiluminescent assay of alkaline phosphatase in human gingival crevicular fluid: investigations with an experimental gingivitis model and studies on the source of the enzyme within crevicular fluid. J Clin Periodontol. 1996: 23; 587-594.

34.     Chapple ILC, Walmsley AD, Moscrop H, Saxby MS. Effect of instrument power setting during ultrasonic scaling upon treatment outcome. J Periodontol. 1995: 66; 756-760.

33.     Chapple ILC, Cross IA, Glenwright HD, Matthews JB. Calibration and reliability of the Periotron 6000 for individual gingival crevicular fluid samples. J Periodont Res. 1995: 30; 73-79.

32.     Chapple ILC, Glenwright HD, Matthews JB, Thorpe GHG, Lumley PJ. Alkaline phosphatase levels in gingival crevicular fluid in health and gingivitis: cross-sectional studies. J Clin Periodontol. 1994: 21; 407-414.

31.     Shah S, Walmsley AD, Chapple ILC, Lumley PJ. Variability of sonic scaling tip measurement. J Clin Periodontol. 1994: 21; 705-709.

30.     Chapple ILC, Thorpe GHG, Glenwright HD, Matthews JB. Chemiluminescent assays for alkaline phosphatase in the diagnosis of the human periodontal diseases.     Bioluminescence & Chemiluminescence - Szalay A, Kricka LJ, Stanley P (Eds), 1993: 281-285.

29.     Chapple ILC. Hypophosphatasia: dental aspects and mode of inheritance. J Clin Periodontol. 1993: 20; 615-622.

28.     Chapple ILC, Matthews JB, Thorpe GHG, Glenwright HD, Smith JM, Saxby M. A new ultrasensitive chemiluminescent assay for the site-specific quantification of alkaline phosphatase in gingival crevicular fluid. J Periodont Res. 1993: 28; 266-273.

27.     Chapple ILC, Walmsley AD, Saxby MS, Moscrop H. The effect of subgingival irrigation with chlorhexidine during ultrasonic scaling. J Periodontol. 1992: 63; 812-816.

26.     Chapple ILC, Thorpe GHG, Smith JM, Saxby MS, Glenwright HD, Green A, Perry GM, Grundy M, Shaw L, Matthews JB.    Hypophosphatasia: a family study involving a case diagnosed from gingival crevicular fluid.   J Oral Pathol Med. 1992: 21; 426-431.

25.     Heasman PA, Jacobs DJ, Chapple ILC.          An evaluation of the effectiveness and patient compliance with plaque control methods. J Clin Prev Dent. 1989: 11; 24-28.

Other Scientific and Clinical Manuscripts:

24.     Wilson N, Patel R, Gallagher J, Chapple ILC. How to select the right mouthwash. The Pharmaceutical Journal. 2014: 292: 119-121.

23.     Chapple ILC, Wilson NHF. Manifesto for a paradigm shift: periodontal health for a better life. British Dental Journal 2014; 216: 159-162.

22.     Blair FM, Chapple IL. Prescribing for periodontal disease. Prim Dent J. 2014: 3; 38-43. doi: 10.1308/205016814813877234.

21.     Kanthimathinathan HK, Browne F, Ramirez R, McKaig S, Debelle G, Martin J, Chapple IL, Kay A, Moss C. Multiple cerebral abscesses in Papillon-Lefèvre syndrome. Childs Nerv Syst. 2013: 29; 1227-9. doi: 10.1007/s00381-013-2152-2.

20.     Parmar S, Chapple IL. Late diagnosis of an occult tumour - what lessons can we learn? Br Dent J. 2012: 212 ; 531-534. doi: 10.1038/sj.bdj.2012.468.

19.     Saha S, Gopakumar A, Chapple ILC. Use of Emdogain in the management of a lateral periodontal cyst: a clinical report. Perio. 2006: 3; 211-215.

18.     Hayes M, Tomson P, Parmar S, Chapple ILC. Proliferative verrucous gingival leukoplakia: a sinister condition. Perio. 2006: 3; 205-210.

17.     Roberts A, Laird WRE, Murphy P, Chapple ILC. Pre-Prosthetic planning for periodontal surgery: a technique report. Perio. 2006: 3; 219-226.

16.     Patanwala A, Fisher E W, Chapple ILC. Plasma cell gingivitis affecting the gingivae, palatal mucosa and laryngeal cords. Perio. 2006: 2; 123-128.

15.     Chapple ILC, Malik K, Freeman R. Self-inflicted gingival trauma in a 13-year-old girl: what are the psychosocial issues that may underlie this behaviour? Perio. 2005: 3; 219-224.

14.     Roberts A, Milward MR, Parmar S, Jephcott A, Chapple ILC. Squamous cell carcinoma arising within gingival lichen planus.     Perio. 2005: 3; 213-218.

13.     Milward MR, El-Shimy N, Roberts A, Chapple ILC/ Multiple or Disseminated Pyogenic Granulomata? A Complex Presentation in a Nine-Year-Old Boy. Perio. 2005: 3; 205-211.

12.     Taibjee SM, Zhang L, Chapple ILC, Thakkar N, Moss C. Pyogenic skin infections as a presentation of Papillon-Lefèvre syndrome: phenotypic variability or under-reporting? Perio. 2005: 3; 191-197.

11.     Baxter A, Roberts A, Shaw L, Chapple IL Localised scleroderma in 12-year-old girl, presenting as gingival recession.  A case report and literature review.  Dent Update. 2001:28; 258-262.

10.     Butterworth CJ, Chapple ILC. Drug-induced gingival overgrowth: a case of auto-correction of incisor drifting. Dent Update. 2001: 28; 411-416.

9.       Chapple ILC, Lumley PJ. Periodontal/endodontic lesions. Dent Update. 1999: 26; 331-341.

8.       Chapple ILC. Periodontal diagnosis: where are we now and where are we going?The GDPA Report. 1998/99: 76-85.

7.       Chapple ILC. Periodontal disease free radicals and antioxidants. Clinical P News 1997:7,31-32

6.       Chapple ILC. Periodontal diseases in children and adolescents: classification, aetiology and management. Dent Update. 1996: 6; 210-216.

5.       Chapple ILC. The use of AMPPD and CSPD in the early diagnosis of the human periodontal diseases. Luminescence Topics, Tropix Publications, 1993: 2; 1.

4.       Chapple ILC, Rout PGJ, Basu MK. Gingival Kaposi's Sarcoma: the first indication of HIV infection. Dent Update. 1992: 19; 296-301.

3.       Chapple ILC, Manogue M. Management of recurrent follicular ameloblastoma. Dent Update. 1991: 18; 309-312.

2.       Chapple ILC, Ord RA. Three cases of patent nasopalatine canals: case reports and a review of the literature. Oral Surg, Oral Med, Oral Pathol. 1990: 69; 554-558.

1.       Millet DT, Chapple ILC, Hirschman P, Corrigan M. Septic osteoradionecrosis of the mandible associated with pathological fracture: report of two cases. J Clin Radiol. 1990: 41; 408-410.