Professor Lars Holm Ph.D., M.Sc.

Professor Lars Holm

School of Sport, Exercise and Rehabilitation Sciences
Professor of Protein Turnover in Translational Physiology
Director of Metabolite Mass Spectrometry Facility

Contact details

Telephone
+44 (0)121 414 6737
Telephone (2)
+45 5360 3180 (Danish) preferred
Email
l.holm@bham.ac.uk
View my research portal
Address
School of Sport, Exercise and Rehabilitation Sciences
University of Birmingham
Edgbaston
Birmingham
B15 2TT
UK

Professor Lars Holm’s science is centred on the acute regulation of and changes in muscle protein turnover rates. Of special focus is the measurement of protein kinetics and metabolite fluxes using stable isotope tracer methodologies. The tracer approaches to assess the turnover rates are adapted to reveal adaptations in muscle mass and extrapolated into other aspects of health and daily living through interdisciplinary research endeavours.

Qualifications

  • Pedagogic education to teach in academia (University of Copenhagen, Denmark).
  • Research Management Course (Copenhagen Business School, Denmark).
  • FELASA license C (No 006/03/610). 

Biography

  • 2017  Professor, School of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, UK.
  • 2013  Associate Professor, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.
  • 2009  Postdoctoral Associate, Institute of Sports Medicine, Bispebjerg Hospital, Copenhagen, Denmark.
  • 2008  Research Associate, Department of Chemistry, University of Vermont, Burlington, Vermont, USA.
  • 2005  Ph.D. student, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.
  • 2002  Research Assistant, Institute of Sports Medicine, Bispebjerg Hospital, Copenhagen, Denmark.
  • 2000  Research student, Institute of Sports Medicine, Bispebjerg Hospital, Copenhagen, Denmark.

Teaching

  • Pre-grad: Lectured and class teaching in courses at the medical education at University of Copenhagen in basal human biology, medical physiology and pathophysiology, and lectured in at the faculty of natural sciences in sarcopenia, human nutrition, human physiology.
  • Post-grad:  Lectured at various Ph.D. courses.
  • Censor (Master and Ph.D.): Sweden, Norway, UK, Denmark. 

Postgraduate supervision

  • Fate of Nutrient-derived Amino Acids: Determined by intrinsically labelled proteins.
  • Counteracting Age-related Loss of Muscle mass, CALM.
  • Impact of energy/protein supplementation in older people on whole body protein turnover and net balance.
  • Impact of carbohydrate on dietary protein uptake and stimulation of muscle protein synthesis.
  • Muscle protein breakdown rate: Its measurement and molecular regulation.
Please, contact me via email with any inquiries about collaboration.

Research

Professor Holms main research interest is to gain knowledge on how factors like dietary protein digestion and amino acid absorption rates as well as whole body and skeletal muscle amino acid fluxes and protein turnover rates are 1) validly measured by stable isotopically labelled substances, and 2) affected by intake of different protein sources and energy intake. Further, the impact of muscular activity and physical activity on whole body and skeletal muscle amino acid fluxes and protein turnover rates is investigated in various conditions. The responses are sought translated into long-term adaptations in muscle protein mass.

The main goal for Professor Holms research is to create evidence and develop strategies for nutritional and/or muscular activity recommendations that can support maintenance and growth or limit loss of muscle mass in older age and during challenged conditions like hospitalization and physio rehabilitation.

The The Milke Levy Fund has granted Prof. Lars Holm to the study entitled Oral Nutritional Supplementation to be conducted in 2019-2020 at School of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, Birmingham, UK.

The main purpose of this study is to investigate how a milk-protein based high energy nutritional supplementation at breakfast and pre-bedtime timepoints affects whole body muscle protein turnover rates in slightly hypocaloric older individuals.

The study is conducted by Ph.D. student Yusuke Nishimura who will carry out the experiments in 2019 and analyse samples in 2019 and 2020. The results from the study will be published in open-access, peer-reviewed scientific journals during 2020 and 2021 and the main outcomes will be accessible for every one being interested via the present web-page. 

Current websites:

Enabling Technologies Mass Spectrometry

Metabolite Mass Spectrometry Facility, SportExR

Publications

  1. Holm L, Reitelseder S, Dideriksen K, Nielsen RH, Bülow J and Kjaer M: “The single biopsy approach in determining protein synthesis in human slow turning over tissue: use of flood-primed continuous infusion of amino acid tracers”. Am J of Physiology, Endocrinology and Metabolism, Innovative Methodology, 306(11): E1330-9, 2014.
  2. Holm L, O’Rourke B, Ebenstein D, Toth MJ,  Bechshoeft R, Holstein-Rathlou NH, Kjaer M and Matthews DE: “Determination of steady state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids: a method applicable in humans”. Am J of Physiology, Endocrinology and Metabolism, Innovative Methodology, 304(8): E895-907, 2013.
  3. Holm L, van Hall G, Rose AJ, Miller BF, Doessing S, Richter EA, and Kjaer M: “Contraction intensity and feeding affect collagen and myofibrillar protein synthesis rates differently in human skeletal muscle”. Am J of Physiology, Endocrinology and Metabolism 298(2): E257-E269, 2010.
  4. Holm L, Reitelseder S, Pedersen TG, Doessing S, Petersen SG, Flyvbjerg A, Andersen JL, Aagaard P, and Kjaer M: ”Changes in muscle size and MHC composition in response to resistance exercise with heavy and light loading intensity”. Journal of Applied Physiology 105(5): 1454-1461, 2008.
  5. Holm L, Olesen JL, Matsumoto K, Doi T, Mizuno M, Alsted TJ, Mackey AL, Schwarz P, and Kjaer M: ”Protein-containing nutrient supplementation following strength training enhances the effect on muscle mass, strength, and bone formation in postmenopausal women”. Journal of Applied Physiology. 105(1): 274-281, 2008.
  6. Holm L and Kjaer M: “Measuring protein breakdown in individual proteins in vivo”. Curr Opin in Clin Nutr and Metab Care, 13: 526-31, 2010.
  7. Bülow J, Ulijaszek S, Holm L: “Rejuvenation of the term Sarcopenia”. J Applied Physiol. Accepted July 9th 2018.
  8. Bechshoeft CL, Rasmussen AB, Schjerling P, Holm L: “Existence of life-time stable proteins in mature rats - dating of proteins' age by repeated short-term exposure to labeled amino acids throughout age”. PLoSOne, Sep 28;12(9), 2017. e0185605.
  9. Agergaard J, Bulow J, Jensen JK, Reitelseder S, Drummond MJ, Schjerling P, Scheike T, Serena A, Holm L: “Light-load resistance exercise increases muscle protein synthesis and hypertrophy signaling in elderly men”. Am J of Physiology: Endocrinology and Metabolism, 312: E326-38, 2017.
  10. Bechshoeft RL, Malmgaard-Clausen NM, Madsen BG, Beyer N, Mackey AL, Andersen JL, Kjaer M, Holm L: “Improved skeletal muscle mass and strength after heavy strength training in very old individuals”. Exp Gerontology, 92, 96-105, 2017.
  11. Bechshoeft RL, Reitelseder S, Højfeldt GW, Castro-Mejia JL, Khakimow B, Bin Ahmad HF, Kjaer M, Engelsen SB, Johansen SMB, Rasmussen MA, Lassen AJ, Jensen T, Beyer N, Serena A, Perez-Cueto FJA, Nielsen DS, Jespersen AP, Holm L: “Counteracting Age-related Loss of Skeletal Muscle Mass: A Clinical and Ethnological Trial on the Role of Protein Supplementation and Training Load (CALM Intervention Study): Study protocol for a randomized controlled trial”. Trials, 17:397, 2016.
  12. Mikkelsen UR, Dideriksen K, Andersen MB, Boesen A, Malmgaard-Clausen NM, Sørensen IJ, Schjerling P, Kjaer M, and Holm L: “Preserved skeletal muscle protein anabolic response to acute exercise and protein intake in well-treated rheumatoid arthritis patients”. Arthritis Research & Therapy, 17:271, 2015; doi: 10.1186/s13075-015-0758-3.
  13. Reitelseder S, Agergaard J, Doessing S, Helmark IC, Schjerling P, van Hall G, Kjaer M, and Holm L: “Positive muscle protein net balance and differential regulation of atrogene expression after resistance exercise and milk protein supplementation”. Eur J Nutr 53(1): 321-33, 2014.
  14. Agergaard J, Reitelseder S, Pedersen TG, Doessing S, Schjerling P, Langberg H, Miller BF, Aagaard P, Kjaer M, and Holm L: “Myogenic, matrix and growth factor mRNA expression in human skeletal muscle: effect of contraction intensity and feeding”. Muscle and Nerve 47(5): 748-59, 2013.
  15. Reitelseder S, Agergaard J, Doessing S, Helmark IC, Kund P, Kristensen NB, Frystyk J, Flyvbjerg A, Schjerling P, van Hall G, Kjaer M, and Holm L: “Whey and casein labelled with L-[1-13C]-leucine and muscle protein synthesis: effect of resistance exercise and protein ingestion”. Am J of Physiology, Endocrinology and Metabolism 300(1): E231-42, 2011.
  16. Hostrup M, Reitelseder S, Jessen S, Kalsen A, Nyberg M, Egelund J, Kreiberg M, Kristensen CM, Thomassen M, Pilegaard H, Backer V, Jacobson G, Holm L, Bangsbo J: ”Beta2-adrenoceptor agonist salbutamol increases protein turnover rates and alters signalling in skeletal muscle after resistance exercise in young men”. J Physiol, accepted June 29th, 2018.
  17. Bülow J, Agergaard J, Kjaer M, Holm L, Reitelseder S: “No additional effect of different types of physical activity on 10-hours muscle protein synthesis in elderly men on a controlled energy and protein sufficient diet”. Exp Gerontology 79: 16-25, 2016.
  18. Bach E, Nielsen RR, Vendelbo MH, Møller AB, Jessen N, Buhl M, Hafstrøm TK, Holm L, Pedersen SB, Pilegaard H, Biensø RS, Jørgensen JOL, Møller N: “Direct effects of TNF-a on local fuel metabolism and cytokine levels in the placebo controlled bilaterally infused human leg: increased insulin sensitivity, increased net protein breakdown and increased IL-6 release”. Diabetes 62(12): 4023-9, 2013.
  19. Mackey AL, Holm L, Reitelseder S, Pedersen TG, Doessing S, Kadi F, and Kjaer M. “Myogenic response of human skeletal muscle to 12 weeks of resistance training at light loading intensity”. Scand J Med Sci in Sports, 21(6): 773-82, 2011.
  20. Mikkelsen UR, Schjerling P, Langberg H, Reitelseder S, Helmark IC, Holm L, Skovgaard D, Kjaer M, and Heinemeier KM. “Local NSAID infusion does not affect protein synthesis and gene expression in human muscle after eccentric exercise”. Scand J Med Sci in Sports, 21(5): 630-44, 2011.

View all publications in research portal