Module descriptions for MSc in Molecular Biotechnology
Introduction to Biotechnology
Module organiser: Dr Peter Lund
Description: This module will teach the practical skills which you require in your lab projects and in a practical modules, revise some areas which some students traditionally find challenging (handling concepts of concentration and dilution), and look in lectures and discussion sessions at the history, development, and current shape of the worldwide biotechnology industry and the employment opportunities within it.
Research Techniques in Molecular Biotechnology
Module Organiser: Dr Tim Dafforn
Semester: 1 and 2
Description: This module is intended to provide in-depth research training. Sessions are designed to give you an appreciation of, and enhance your knowledge of, modern techniques in molecular and cellular biology, for all of which the University of Birmingham has world-class facilities. Members of staff present details of different techniques, with examples including animal cell culture; high throughput DNA sequencing; cell manipulation, transfection & micro-injection; protein expression and purification; microscopy and cell sorting; circular dichroism, fluorescence & absorbance; reporter gene technology; immunodiagnostics; NMR spectroscopy; mass spectrometry; and X-Ray crystallography. Some sessions will include tours of the laboratories and a chance to work on data generated using the above methods. Workshops tackle open-ended research problems using some of the above methods.
Practical Applications of Molecular Biotechnology
Module Organiser: Prof Jeff Cole
Description: This continuously assessed practical module will focus on techniques used in molecular biotechnology. The first of two major groups of activities will involve preliminary experiments to learn the basic techniques necessary to prepare fermentation experiments designed to produce a difficult recombinant protein. Then students will work in teams of 4 or 5 to complete a fermentation experiment. They will individually write reports of each group of experiments, the second of which must be presented in the form of a paper suitable for submission to a specified journal. The assessments will be based on all four components, the performance in the laboratory, and the quality of the written reports,
Gene Expression Analysis
Module Organiser: Professor Zewei Luo
Description: This module introduces the concepts and methods of genomics, transcriptomics and proteomics, and provides an in depth coverage of their current status. A special emphasis is given to genetical genomics and reconstruction of transcriptional networks, and the analysis and interpretation of micro-array data.
From Bench to Market – the development of pharmaceutical drug products
Module Organiser: Dr Rachel Bridson (Chemical Engineering)
Description: The module covers the typical “time-line” of drug product development, Key activities, issues and importance of stages in drug product development: drug discovery, developability, pre-clinical studies, formulation, clinical trials, primary and secondary manufacturing, marketing and post marketing activities, Patents and intellectual property, The regulatory and quality environment of the pharmaceutical industry, and quality by design approaches to pharmaceutical manufacturing.
Module Organiser: Dr Jon Green
Description: This module describes the different types of therapeutic biologicals (also known as biopharmaceuticals) used for diagnosis, prevention and treatment of serious and chronic diseases. These molecules are generally large, complex modified proteins derived from living material. The module will cover the production and use of therapeutic monoclonal antibodies, cytokines, hormones, enzymes and vaccines. The production of these molecules, their mode of action and pre-clinical development will be described.
Module Organiser: Dr Eva Hyde
Description: In this module you engage in an activity designed to develop your entrepreneurial and business skills in a science context and develop team working skills. A mini-course is held on writing grants and business plans, after which you work in small groups on a topic of your choice for an application. A series of presentations and feedback sessions lead to final presentations where each group presents their proposal or business plan to the other students, who have to provide a written critique of the proposal.
Functional Genomics and Reverse Genetics
Module Organiser: Dr Matthias Soller
Description: This module will explain current methods of exploring and confirming the nature of candidate genes underlying quantitative and major gene traits. It will cover functional genomic and molecular genetic techniques including forward and reverse genetic approaches, gene cloning, functional analysis of genes via knock-out and knock-in technologies, and techniques for analysing protein function within an organism. We will also cover more general topics relevant to modern functional genomics "in practice”.
Project titles offered to MSc Molecular Biotechnology students 2014/15
- Investigation of whether cell stress induces nuclear translation
- Understanding the links between translation and transcription
- Production, purification and biochemical functions of two proteins implicated in the resistance of E.coli to nitrosative stress
- Investigating the effect of cancer mutations on the activity of a ribosomal hydroxylase
- Imaging with liquid extraction surface analysis and ion mobility mass spectrometry for probing protein chemistry
- Characterisation of pro-survival kinases in the breat cancer cell line SUM52
- Understanding regulation of apoptosis-induced cell proliferation in cancer and tissue regeneration models
- Turning gene regulation inside out: prevalence of promoters within genes in bacterial chromosomes
- Impact of the host environment on fungal phagocytosis
- Generation of molecular tools to investigate brain development in the fruit-fly Drosophila
- Is the activity of LAR phosphatase required for specific recruitment of proteins to the PDGF receptor?
- Examining the effect of azide on oxidative stress in E.coli
- Determining whether AKR1C3 selective inhibitors can sensitise tumour cells to chemotheraphy
- Investigating the role of quorum sensing agonists on Vibro cholera disease transmission
- Transfer of antibiotic resistance carrying plasmids in spatially structured multispecies communities
- Analysis of gene expression variation in E. coli
- Acid sensing in E.coli: the roles of the EvgS protein
- Analysis of genes responsible for acid stress resistance in E. coli
- Quantitative genetic analysis in Arabidopsis
- New Bioanomaterials for Cleaning up Radioactive Contamination in Waters and Soil Washings
- Using Solar Energy Effectively for Boosting Photosynthetic Biotechnologies
- Role of epigenetic mechanisms in regulating tissue-specific transcriptional responses to stressors in Daphnia magna
- Analysis of alternative splicing in the hypervariable immune and axon guidance receptor Dscam
- Crispr/Cas9 mediated genome editing for the analysis of ELAV/Hu regulated post-transcriptional gene networks
- Investigations into the dynamics of glycogen metabolism
- Identifying the role of PHD1 in cellular oxygen sensing
- Dissecting plasmid host range determinants
- Regulation of antibiotic production in marine bacteria
- Regulation of the scavenger receptor CD36 by tetraspanins
- Bio-informatic analysis of a new dataset to explore the ways in which human cells resist chemotherapeutic toxins
- Overcoming the natural resistance of human cells to chemotherapeutic drugs with novel inhibitor combinations
- Structural Bioinformatics for the Reengineering of the Mupirocin and Thiomarinol Antibiotic Biosynthesis Pathways