LC-MS based metabolomics and metabolite identification with the Q Exactive™ Plus and Orbitrap ID-X™ Tribrid™

School of Biosciences - University of Birmingham
Event cost
Monday 15 May (09:15) - Thursday 18 May 2023 (16:00)

 PCB scientists servicing a mass spectrometer

LC-MS based metabolomics and metabolite identification with the Q Exactive™ Plus and Orbitrap ID-X™ Tribrid™

Tickets are now sold out for our May 2023 course



Course Overview

This 4-day course introduces LC-MS based metabolomics and metabolite identification using both the Q Exactive Plus (QE+) and Orbitrap ID-X Tribrid mass spectrometers, guiding delegates through experimental design, sample preparation, using the QE+ and ID-X, data processing, univariate and multivariate data analysis, and metabolite annotation/identification. It is an intensive course designed to provide foundational knowledge for delegates to develop further skills and undertake their own metabolomics experiments. 

This course is led and delivered by five experts in the field of metabolomics and includes lectures, hands-on laboratory sessions, and computer workshops to provide a detailed overview of the metabolomics pipeline.

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Topics Covered

  • Introduction to QE+ and ID-X mass spectrometers and the metabolomics workflow
  • Experimental design and the importance of quality control (QC) samples
  • Sample preparation including extraction of polar metabolites and lipids from biofluids (urine and plasma) and tissue samples
  • Hands-on data acquisition for untargeted metabolomics and hybrid metabolomics, the latter combining targeted and untargeted measurements
  • Setting up the Vanquish UHPLC coupled to the QE+
  • Types of data which can be collected on the QE+ and and ID-X (m/z, retention time, MS/MS, MSn)
  • MS/MS and MSn experiments
  • Data processing workshop
  • Data analysis workshop (univariate and multivariate analysis)
  • Introduction to metabolite identification applying Data Dependent Analysis
  • Conversion of raw data to molecular formula and putative metabolite annotations
  • Mass spectral libraries (using mzCloud), and how to search them
  • Tools for mass spectral interpretation
  • Reporting standards for metabolite identification
  • Question and answer session with a panel of experts

Preview the full course programme here



The course is aimed at individuals with minimal previous experience of applying LC-MS in metabolomics studies. A basic working knowledge of LC-MS would be highly advantageous.


Early-bird: £1,349

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Standard: £1,810

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MRC Bursary: £0

Applications Open


Please read the Birmingham Metabolomics Training Centre's Conditions and Cancellation Policy prior to registering.

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