India Institute Fellow in the School of Geography, Earth and Environmental Sciences
A successful senior research fellow at ICGEB, New Delhi, Surbhi works on the role of autophagy in regulating homeostasis and antibacterial mechanisms in macrophages. During her fellowship at the Institute of Cancer and Genomic Sciences she is undertaking a short-term research project that will identify compounds influencing autophagy, which is a biological process essential for cellular homeostasis, in human pluripotent stem cell-derived macrophages. The objectives are to undertake an image-based chemical screen to identify potent autophagy modulators that are effective in human macrophages, and further characterize the screen hits and the target pathways for their effects on autophagy. This will be important to understand how autophagy is regulated in macrophages for therapeutic applications. The research will involve cell culture of human pluripotent stem cells and their differentiation into monocytic cells and macrophages, high-content microscopy, confocal microscopy, immunoblotting, immunofluorescence, and autophagy and apoptosis assays.