3i's Marianas Light Sheet (diSPIM) Microscope

3is Marianas Light Sheet Microscope

Selective plane illumination microscopy (SPIM) on the Marianas Light Sheet uses a thin sheet of light to illuminate only the plane of interest, reducing phototoxicity by drastically cutting total light dose and allowing for prolonged specimen imaging. 

The system captures two alternating views of a sample which are then merged to generate an even 3D image without distortion, and is suitable for imaging cellular and subcellular structures in larger live samples (tissue sections, spheroids/organoids, small organisms). 

Microscope Details

  • Sample size: cells and small tissue, embryos, organoids, etc.
  • Main application: rapid 3D imaging with isotropic resolution and low phototoxicity
  • Modalities: Light sheet selective plane illumination microscopy (SPIM) 
  • Spatial Resolution: isotropic 330 nm x, y, z 
  • Environmental control: Temperature, humidity and CO2
  • Imaging media: Aqueous media only 
  • Excitation Lasers: 488, 561 and 640 nm